Starch encapsulation

ABSTRACT

Hybrid polypeptides are provided formed with encapsulating regions from genes that encode for anabolic proteins. More particularly, the present invention relates to recombinant nucleic acid molecules that code for genes which encapsulate an attached protein within a matrix; preferably, these genes encapsulate a desired (&#34;payload&#34;) polypeptide within starch, and more specifically within the starch granule matrix. Expression vectors comprising these recombinant nucleic acid molecules, and hosts therefor, and more specifically the starch-bearing portions of such hosts, transformed with such vectors, are also provided. Preferably, grain containing a foreign protein encapsulated within the starch is provided, useful to produce mammalian, fish and avian food. The invention also encompasses methods of producing purified protein from starch and particularly from starch granules, and industrial uses of such protein.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application claims priority to provisional patent application Ser. No. 60/026,855 filed Sep. 30, 1996. Said provisional application is incorporated herein by reference to the extent not inconsistent herewith.

BACKGROUND OF THE INVENTION

Polysaccharide Enzymes

Both prokaryotic and eukaryotic cells use polysaccharide enzymes as a storage reserve. In the prokaryotic cell the primary reserve polysaccharide is glycogen. Although glycogen is similar to the starch found in most vascular plants it exhibits different chain lengths and degrees of polymerization. In many plants, starch is used as the primary reserve polysaccharide. Starch is stored in the various tissues of the starch bearing plant. Starch is made of two components in most instances; one is amylose and one is amylopectin. Amylose is formed as linear glucans and amylopectin is formed as branched chains of glucans. Typical starch has a ratio of 25% amylose to 75% amylopectin. Variations in the amylose to amylopectin ratio in a plant can effect the properties of the starch. Additionally starches from different plants often have different properties. Maize starch and potato starch appear to differ due to the presence or absence of phosphate groups. Certain plant' starch properties differ because of mutations that have been introduced into the plant genome. Mutant starches are well known in maize, rice and peas and the like.

The changes in starch branching or in the ratios of the starch components result in different starch characteristic. One characteristic of starch is the formation of starch granules which are formed particularly in leaves, roots, tubers and seeds. These granules are formed during the starch synthesis process. Certain synthases of starch, particularly granule-bound starch synthase, soluble starch synthases and branching enzymes are proteins that are "encapsulated" within the starch granule when it is formed.

The use of cDNA clones of animal and bacterial glycogen synthases are described in International patent application publication number GB92/01881. The nucleotide and amino acid sequences of glycogen synthase are known from the literature. For example, the nucleotide sequence for the E. coli glgA gene encoding glycogen synthase can be retrieved from the GenBank/EMBL (SWISSPROT) database, accession number J02616 (Kumar et al., 1986, J. Biol. Chem., 261:16256-16259). E. coli glycogen biosynthetic enzyme structural genes were also cloned by Okita et al. (1981, J. Biol. Chem., 256(13):6944-6952). The glycogen synthase glgA structural gene was cloned from Salmonella typhimurium LT2 by Leung et al. (1987, J. Bacteriol., 169(9):4349-4354). The sequences of glycogen synthase from rabbit skeletal muscle (Zhang et al., 1989, FASEB J., 3:2532-2536) and human muscle (Browner et al., 1989, Proc. Natl. Acad. Sci., 86:1443-1447) are also known.

The use of cDNA clones of plant soluble starch synthases has been reported. The amino acid sequences of pea soluble starch synthase isoforms I and II were published by Dry et al. (1991, Plant Journal, 2:193202). The amino acid sequence of rice soluble starch synthase was described by Baba et al. (1993, Plant Physiology, ). This last sequence (rice SSTS) incorrectly cites the N-terminal sequence and hence is misleading. Presumably this is because of some extraction error involving a protease degradation or other inherent instability in the extracted enzyme. The correct N-terminal sequence (starting with AELSR) is present in what they refer to as the transit peptide sequence of the rice SSTS.

The sequence of maize branching enzyme I was investigated by Baba et al., 1991, BBRC, 181:8794. Starch branching enzyme II from maize endosperm was investigated by Fisher and Shrable (993, Plant Physiol., 102:10451046). The use of cDNA clones of plant, bacterial and animal branching enzymes have been reported. The nucleotide and amino acid sequences for bacterial branching enzymes (BE) are known from the literature. For example, Kiel et al. cloned the branching enzyme gene glgB from Cyanobacterium synechococcussp PCC7942 (1989, Gene (Amst), 78(1):918) and from Bacillus stearothermophilus (Kiel et al., 1991, Mol. Gen. Genet., 230(12):136-144). The genes glc3 and gha1 of S. cerevisiae are allelic and encode the glycogen branching enzyme (Rowen et al., 1992, Mol. Cell Biol., 12(1):22-29). Matsumomoto et al. investigated glycogen branching enzyme from Neurospora crassa (1990, J. Biochem., 107:118-122). The GenBank/EMBL database also contains sequences for the E. coli glgB gene encoding branching enzyme.

Starch synthase (EC 2.4.1.11) elongates starch molecules and is thought to act on both amylose and amylopectin. Starch synthase (STS) activity can be found associated both with the granule and in the stroma of the plastid. The capacity for starch association of the bound starch synthase enzyme is well known. Various enzymes involved in starch biosynthesis are now known to have differing propensities for binding as described by Mu-Forster et al. (1996, plant Phys. 111: 821-829). Granule-bound starch synthase (GBSTS) activity is strongly correlated with the product of the waxy gene (Shure et al., 1983, Cell 35: 225-233). The synthesis of amylose in a number of species such as maize, rice and potato has been shown to depend on the expression of this gene (Tsai, 1974, Biochem Gen 11: 83-96; Hovenkamp-Hermelink et al., 1987, Theor. Appl. Gen. 75: 217-221). Visser et al. described the molecular cloning and partial characterization of the gene for granule-bound starch synthase from potato (1989, Plant Sci. 64(2):185192). Visser et al. have also described the inhibition of the expression of the gene for granule-bound starch synthase in potato by antisense constructs (1991, Mol. Gen. Genet. 225(2):289296).

The other STS enzymes have become known as soluble starch synthases, following the pioneering work of Frydman and Cardini (Frydman and Cardini, 1964, Biochem. Biophys. Res. Communications 17: 407-411). Recently, the appropriateness of the term "soluble" has become questionable in light of discoveries that these enzymes are associated with the granule as well as being present in the soluble phase (Denyer et al., 1993, Plant J. 4: 191-198; Denyer et al., 1995, Planta 97: 57-62; Mu-Forster et al., 1996, Plant Physiol. 111: 821-829). It is generally believed that the biosynthesis of amylopectin involves the interaction of soluble starch synthases and starch branching enzymes. Different isoforms of soluble starch synthase have been identified and cloned in pea (Denyer and Smith, 1992, Planta 186: 609-617; Dry et al., 1992, Plant Journal, 2: 193-202), potato (Edwards et al., 1995, Plant Physiol 112: 89-97; Marshall et al., 1996, Plant Cell 8: 1121-1135) and in rice (Baba et al., 1993, Plant Physiol. 103: 565-573), while barley appears to contain multiple isoforms, some of which are associated with starch branching enzyme (Tyynela and Schulman, 1994, Physiol. Plantarum 89: 835-841). A common characteristic of STS clones is the presence of a KXGGLGDV consensus sequence which is believed to be the ADP-Glc binding site of the enzyme (Furukawa et al., 1990, J Biol Chem. 265: 2086-2090; Furukawa et al., 1993, J. Biol. Chem. 268: 23837-23842).

In maize, two soluble forms of STS, known as isoforms I and II, have been identified (Macdonald and Preiss, 1983, Plant Physiol. 73: 175-178; Boyer and Preiss, 1978, Carb. Res. 61: 321-334; Pollock and Preiss, 1980, Arch Biochem. Biophys. 204: 578-588; Macdonald and Preiss, 1985 Plant Physiol. 78: 849-852; Dang and Boyer, 1988, Phytochemistry 27: 1255-1259; Mu et al., 1994, Plant J. 6: 151-159), but neither of these has been cloned. STSI activity of maize endosperm was recently correlated with a 76-kDa polypeptide found in both soluble and granule-associated fractions (Mu et al., 1994, Plant J. 6: 151-159). The polypeptide identity of STSII remains unknown. STSI and II exhibit different enzymological characteristics. STSI exhibits primer-independent activity whereas STSII requires glycogen primer to catalyze glucosyl transfer. Soluble starch synthases have been reported to have a high flux control coefficient for starch deposition (Jenner et al., 1993, Aust. J. Plant Physiol. 22: 703-709; Keeling et al., 1993, Planta 191: 342-348) and to have unusual kinetic properties at elevated temperatures (Keeling et al., 1995, Aust. J. Plant Physiol. 21 807-827). The respective isoforms in maize exhibit significant differences in both temperature optima and stability.

Plant starch synthase (and E. Coli glycogen synthase) sequences include the sequence KTGGL which is known to be the ADPG binding domain. The genes for any such starch synthase protein may be used in constructs according to this invention.

Branching enzyme [α1,4Dglucan: α1,4Dglucan 6D(α1,4Dglucano) transferase (E.C. 2.4.1.18)], sometimes called Q-enzyme, converts amylose to amylopectin. A segment of a α1,4Dglucan chain is transferred to a primary hydroxyl group in a similar glucan chain.

Bacterial branching enzyme genes and plant sequences have been reported (rice endosperm: Nakamura et al., 1992, Physiologia Plantarum, 84:329-335 and Nakamura and Yamanouchi, 1992, Plant Physiol., 99:1265-1266; pea: Smith, 1988, Planta, 175:270-279 and Bhattacharyya et al., 1989, J. Cell Biochem., Suppl. 13D:331; maize endosperm: Singh and Preiss, 1985, Plant Physiology, 79:34-40; VosScherperkeuter et al., 1989, Plant Physiology, 90:75-84; potato: Kossmann et al., 1991, Mol. Gen. Genet., 230(12):39-44; cassava: Salehuzzaman and Visser, 1992, Plant Mol Biol, 20:809-819).

In the area of polysaccharide enzymes there are reports of vectors for engineering modification in the starch pathway of plants by use of a number of starch synthesis genes in various plant species. That some of these polysaccharide enzymes bind to cellulose or starch or glycogen is well known. One specific patent example of the use of a polysaccharide enzyme shows the use of glycogen biosynthesis enzymes to modify plant starch. In U.S. Pat. No. 5,349,123 to Shewmaker a vector containing DNA to form glycogen biosynthetic enzymes within plant cells is taught. Specifically, this patent refers to the changes in potato starch due to the introduction of these enzymes. Other starch synthesis genes and their use have also been reported.

Hybrid (fusion) Peptides

Hybrid proteins (also called "fusion proteins") are polypeptide chains that consist of two or more proteins fused together into a single polypeptide. Often one of the proteins is a ligand which bind to a specific receptor cell. Vectors encoding fusion peptides are primarily used to produced foreign proteins through fermentation of microbes. The fusion proteins produced can then be purified by affinity chromatography. The binding portion of one of the polypeptides is used to attach the hybrid polypeptide to an affinity matrix. For example, fusion proteins can be formed with beta galactosidase which can be bound to a column. This method has been used to form viral antigens.

Another use is to recover one of the polypeptides of the hybrid polypeptide. Chemical and biological methods are known for cleaving the fused peptide. Low pH can be used to cleave the peptides if an acid-labile aspartyl-proline linkage is employed between the peptides and the peptides are not affected by the acid. Hormones have been cleaved with cyanobromide. Additionally, cleavage by site-specific proteolysis has been reported. Other methods of protein purification such as ion chromatography have been enhanced with the use of polyarginine tails which increase overall basicity of the protein thus enhancing binding to ion exchange columns.

A number of patents have outlined improvements in methods of making hybrid peptides or specific hybrid peptides targeted for specific uses. U.S. Pat. No. 5,635,599 to Pastan et al. outlines an improvement of hybrid proteins. This patent reports a circularly permuted ligand as part of the hybrid peptide. This ligand possesses specificity and good binding affinity. Anorher improvement in hybrid proteins is reported in U.S. Pat. No. 5,648,244 to Kuliopulos. This patent describes a method for producing a hybrid peptide with a carrier peptide. This nucleic acid region, when recognized by a restriction endonuclease, creates a nonpalindromic 3-base overhang. This allows the vector to be cleaved.

An example of a specifically targeted hybrid protein is reported in U.S. Pat. No. 5,643,756. This patent reports a vector for expression of glycosylated proteins in cells. This hybrid protein is adapted for use in proper immunoreactivity of HIV gp120. The isolation of gp120 domains which are highly glycosylated is enhanced by this reported vector.

U.S. Pat. Nos. 5,202,247 and 5,137,819 discuss hybrid proteins having polysaccharide binding domains and methods and compositions for preparation of hybrid proteins which are capable of binding, to a polysaccharide matrix. U.S. Pat. No. 5,202,247 specifically teaches a hybrid protein linking a cellulase binding region to a peptide of interest. The patent specifies that the hybrid protein can be purified after expression in a bacterial host by affinity chromatography on cellulose.

The development of genetic engineering techniques has made it possible to transfer genes from various organisms and plants into other organisms or plants. Although starch has been altered by transformation and mutagenesis in the past there is still a need for further starch modification. To this end vectors that provide for encapsulation of desired amino acids or peptides within the starch and specifically within the starch granule are desirable. The resultant starch is modified and the tissue from the plant carrying the vector is modified.

SUMMARY OF THE INVENTION

This invention provides a hybrid polypeptide comprising a starch-encapsulating region (SER) from a starch-binding enzyme fused to a payload polypeptide which is not endogenous to said starch-encapsulating region, i.e. does not naturally occur linked to the starch-encapsulating region. The hybrid polypeptide is useful to make modified starches comprising the payload polypeptide. Such modified starches may be used to provide grain feeds enriched in certain amino acids. Such modified starches are also useful for providing polypeptides such as hormones and other medicaments, e.g. insulin, in a starch-encapsulated form to resist degradation by stomach acids. The hybrid polypeptides are also useful for producing the payload polypeptides in easily-purified form. For example, such hybrid polypeptides produced by bacterial fermentation, or in grains or animals, may be isolated and purified from the modified starches with which they are associated by art-known techniques.

The term "polypeptide" as used herein means a plurality of identical or different amino acids, and also encompasses proteins.

The term "hybrid polypeptide" means a polypeptide composed of peptides or polypeptides from at least two different sources, e.g. a starch-encapsulating region of a starch-binding enzyme, fused to another polypeptide such as a hormone, wherein at least two component parts of the hybrid polypeptide do not occur fused together in nature.

The term "payload polypeptide" means a polypeptide not endogenous to the starch-encapsulating region whose expression is desired in association with this region to express a modified starch containing the payload polypeptide.

When the payload polypeptide is to be used to enhance the amino acid content of particular amino acids in the modified starch, it preferably consists of more than three different types of amino acids selected from the group consisting of: Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, and Val.

When the payload polypeptide is to be used to supply a biologically active polypeptide to either the host organism or another organism, the payload polypeptide may be a biologically active polypeptide such as a hormone, e.g., insulin, a growth factor, e.g. somatotropin, an antibody, enzyme, immunoglobulin, or dye, or may be a biologically active fragment thereof as is known to the art. So long as the polypeptide has biological activity, it does not need to be a naturally-occurring polypeptide, but may be mutated, truncated, or otherwise modified. Such biologically active polypeptides may be modified polypeptides, containing only biologically-active portions of biologically-active polypeptides. They may also be amino acid sequences homologous to naturally-occurring biologically-active amino acid sequences (preferably at least about 75% homologous) which retain biological activity.

The starch-encapsulating region of the hybrid polypeptide may be a starch-encapsulating region of any starch-binding enzyme known to the art, e.g. an enzyme selected from the group consisting of soluble starch synthase I, soluble starch synthase II, soluble starch synthase III, granule-bound starch synthase, branching enzyme I, branching enzyme IIa, branching enzyme IIBb and glucoamylase polypeptides.

When the hybrid polypeptide is to be used to produce payload polypeptide in pure or partially purified form, the hybrid polypeptide preferably comprises a cleavage site between the starch-encapsulating region and the payload polypeptide. The method of isolating the purified payload polypeptide then includes the step of contacting the hybrid polypeptide with a cleaving agent specific for that cleavage site.

This invention also provides recombinant nucleic acid (RNA or DNA) molecules encoding the hybrid polypeptides. Such recombinant nucleic acid molecules preferably comprise control sequences adapted for expression of the hybrid polypeptide in the selected host. The term "control sequences" includes promoters, introns, preferred codon sequences for the particular host organism, and other sequences known to the art to affect expression of DNA or RNA in particular hosts. The nucleic acid sequences encoding the starch-encapsulating; region and the payload polypeptide may be naturally-occurring nucleic acid sequences, or biologically-active fragments thereof, or may be biologically-active sequences homologous to such sequences, preferably at least about 75% homologous to such sequences.

Host organisms include bacteria, plants, and animals. Preferred hosts are plants. Both monocotyledonous plants (monocots) and dicotyledonous plants (dicots) are useful hosts for expressing the hybrid polypeptides of this invention.

This invention also provides expression vectors comprising the nucleic acids encoding the hybrid proteins of this invention. These expression vectors are used for transforming the nucleic acids into host organisms and may also comprise sequences aiding in the expression of the nucleic acids in the host organism. The expression vectors may be plasmids, modified viruses, or DNA or RNA molecules, or other vectors useful in transformation systems known to the art.

By the methods of this invention, transformed cells are produced comprising the recombinant nucleic acid molecules capable of expressing the hybrid polypeptides of this invention. These may prokaryotic or eukaryotic cells from one-celled organisms, plants or animals. They may be bacterial cells from which the hybrid polypeptide may be harvested. Or, they may be plant cells which may be regenerated into plants from which the hybrid polypeptide may be harvested, or, such plant cells may be regenerated into fertile plants with seeds containing the nucleic acids encoding the hybrid polypeptide. In a preferred embodiment, such seeds contain modified starch comprising the payload polypeptide.

The term "modified starch" means the naturally-occurring starch has been modified to comprise the payload polypeptide.

A method of targeting digestion of a payload polypeptide to a particular phase of the digestive process, e.g., preventing degradation of a payload polypeptide in the stomach of an animal, is also provided comprising feeding the animal a modified starch of this invention comprising the payload polypeptide, whereby the polypeptide is protected by the starch from degradation in the stomach of the animal. Alternatively, the starch may be one known to be digested in the stomach to release the payload polypeptide there.

Preferred recombinant nucleic acid molecules of this invention comprise DNA encoding starch-encapsulating regions selected from the starch synthesizing gene sequences set forth in the tables hereof.

Preferred plasmids of this invention are adapted for use with specific hosts. Plasmids comprising a promoter, a plastid-targeting sequence, a nucleic acid sequence encoding a starch-encapsulating region, and a terminator sequence, are provided herein. Such plasmids are suitable for insertion of DNA sequences encoding payload polypeptides and starch-encapsulating regions for expression in selected hosts.

Plasmids of this invention can optionally include a spacer or a linker unit proximate the fusion site between nucleic acids encoding the SER and the nucleic acids encoding the payload polypeptide. This invention includes plasmids comprising promoters adapted for a prokaryotic or eukaryotic hosts. Such promoters may also be specifically adapted for expression in monocots or in dicots.

A method of forming peptide-modified starch of this invention includes the steps of supplying a plasmids having a promoter associated with a nucleic acid sequence encoding a starch-encapsulating region, the nucleic acid sequence encoding the starch-encapsulating region being connected to a nucleic acid region encoding a payload polypeptide, and transforming a host with the plasmid whereby the host expresses peptide-modified starch.

This invention furthermore comprises starch-bearing grains comprising: an embryo, nutritive tissues; and, modified starch granules having encapsulated therein a protein that is not endogenous to starch granules of said grain which are not modified. Such starch-bearing grains may be grains wherein the embryo is a maize embryo, a rice embryo, or a wheat embryo.

All publications referred to herein are incorporated by reference to the extent not inconsistent herewith.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1a shows the plasmid pEXS114 which contains the synthetic GFP (Green Fluorescent Protein) subcloned into pBSK from Stratagene.

FIG. 1b shows the plasmid pEXS115.

FIG. 2a shows the waxy gene with restriction sites subcloned into a commercially available plasmid.

FIG. 2b shows the p ET-21A plasmid commercially available from Novagen having the GFP fragment from pEXS 115 subcloned therein.

FIG. 3a shows pEXS114 subcloned into pEXSWX, and the GFP-FLWX map.

FIG. 3b shows the GFP-Bam HIWX plasmid.

FIG. 4 shows the SGFP fragment of pEXS115 subcloned into pEXSWX, and the GFP-NcoWX map.

FIG. 5 shows a linear depiction of a plasmid that is adapted for use in monocots.

FIG. 6 shows the plasmid pEXS52.

FIG. 7 shows the six introductory plasmids used to form pEXS51 and pEX560.

FIG. 7a shows pEXS adh1.

FIG. 7b shows pEXS adh1-nos3'.

FIG. 7c shows pEXS33.

FIG. 7d shows pEXS10zp.

FIG. 7e shows pEXS10zp-adh1.

FIG. 7f shows pEXS10zp-adh1-nos3'.

FIGS. 8a and 8b show the plasmids pEXS50 and pEXS51, respectively, containing the MS-SIII gene which is a starch-soluble synthase gene.

FIG. 9a shows the plasmid pEXS60 which excludes the intron shown in pEXS50, and

FIG. 9b shows the plasmid pEXS61 which excludes the intron shown in pEXS60.

DETAILED DESCRIPTION

The present invention provides, broadly, a hybrid polypeptide, a method for making a hybrid polypeptide, and nucleic acids encoding the hybrid polypeptide. A hybrid polypeptide consists of two or more subparts fused together into a single peptide chain. The subparts can be amino acids or peptides or polypeptides. One of the subparts is a starch-encapsulating region. Hybrid polypeptides may thus be targeted into starch granules produced by organisms expressing the hybrid polypeptides.

A method of making the hybrid polypeptides within cells involves the preparation of a DNA construct comprising at least a fragment of DNA encoding a sequence which functions to bind the expression product of attached DNA into a granule of starch, ligated to a DNA sequence encoding the polypeptide of interest (the payload polypeptide). This construct is expressed within a eukaryotic or prokaryotic cell. The hybrid polypeptide can be used to produce purified protein or to immobilize a protein of interest within the protection of a starch granule, or to produce grain that contains foreign amino acids or peptides.

The hybrid polypeptide according to the present invention has three regions.

    ______________________________________                                         Payload Peptide                                                                              Central Site                                                                             Starch-encapsulating                                     (X) (CS)* region (SER)                                                       ______________________________________                                          X is any amino acid or peptide of interest.                                    * optional component.                                                    

The gene for X can be placed in the 5' or 3' position within the DNA construct described below.

CS is a central site which may be a leaving site, a cleavage site, or a spacer, as is known to the art. A cleavage site is recognized by a cleaving enzyme. A cleaving enzyme is an enzyme that cleaves peptides at a particular site. Examples of chemicals and enzymes that have been employed to cleave polypeptides include thrombin, trypsin, cyanobromide, formic acid, hydroxyl amine, collagenase, and alasubtilisin. A spacer is a peptide that joins the peptides comprising the hybrid polypeptide. Usually it does not have any specific activity other than to join the peptides or to preserve some minimum distance or to influence the folding, charge or water acceptance of the protein. Spacers may be any peptide sequences not interfering with the biological activity of the hybrid polypeptide.

The starch-encapsulating region (SER) is the region of the subject polypeptide that has a binding affinity for starch. Usually the SER is selected from the group consisting of peptides comprising starch-binding regions of starch synthases and branching enzymes of plants, but can include starch binding domains from other sources such as glucoamylase and the like. In the preferred embodiments of the invention, the SER includes peptide products of genes that naturally occur in the starch synthesis pathway. This subset of preferred SERs is defined as starch-forming encapsulating regions (SFER). A further subset of SERs preferred herein is the specific starch-encapsulating regions (SSER) from the specific enzymes starch synthase (STS), granule-bound starch synthase (GBSTS) and branching enzymes (BE) of starch-bearing plants. The most preferred gene product from this set is the GBSTS. Additionally, starch synthase I and branching enzyme II are useful gene products. Preferably, the SER (and all the subsets discussed above) are truncated versions of the full length starch synthesizing enzyme gene such that the truncated portion includes the starch-encapsulating region.

The DNA construct for expressing the hybrid polypeptide within the host, broadly is as follows:

    ______________________________________                                                         Transit Peptide                                                  Promoter Intron* Coding Region* X SER Terminator                             ______________________________________                                          *optional component. Other optional components can also be used.         

As is known to the art, a promoter is a region of DNA controlling transcription. Different types of promoters are selected for different hosts. Lac and T7 promoters work well in prokaryotes, the 35S CaMV promoter works well in dicots, and the polyubiquitin promoter works well in many monocots. Any number of different promoters are known to the art and can be used within the scope of this invention.

Also as is known to the art, an intron is a nucleotide sequence in a gene that does not code for the gene product. One example of an intron that often increases expression in monocots is the Adh1 intron. This component of the construct is optional.

The transit peptide coding region is a nucleotide sequence that encodes for the translocation of the protein into organelles such as plastids. It is preferred to choose a transit peptide that is recognized and compatible with the host in which the transit peptide is employed. In this invention the plastid of choice is the amyloplast.

It is preferred that the hybrid polypeptide be located within the amyloplast in cells such as plant cells which synthesize and store starch in amyloplasts. If the host is a bacterial or other cell that does not contain an amyloplast, there need not be a transit peptide coding region.

A terminator is a DNA sequence that terminates the transcription.

X is the coding region for the payload polypeptide, which may be any polypeptide of interest, or chains of amino acids. It may have up to an entire sequence of a known polypeptide or comprise a useful fragment thereof. The payload polypeptide may be a polypeptide, a fragment thereof, or biologically active protein which is an enzyme, hormone, growth factor, immunoglobulin, dye, etc. Examples of some of the payload polypeptides that can be employed in this invention include, but are not limited to, prolactin (PRL), serum albumin, growth factors and growth hormones, i.e., somatotropin. Serum albumins include bovine, ovine, equine, avian and human serum albumin. Growth factors include epidermal growth factor (EGF), insulin-like growth factor I (IGF-I), insulin-like growth factor II: (IGF-II), fibroblast growth factor (FGF), transforming growth factor alpha (TGF-alpha), transforming growth factor beta (TGF-beta), nerve growth factor (NGF), platelet-derived growth factor (PDGF), and recombinant human insulin-like growth factors I (rHuIGF-Il) and II (rHuIGF-II). Somatotropins which can be employed to practice this invention include, but are not limited to, bovine, porcine, ovine, equine, avian and human somatotropin. Porcine somatotropin includes delta-7 recombinant porcine somatotropin, as described and claimed in European Patent Application Publication No. 104,920 (Biogen). Preferred payload polypeptides are somatotropin, insulin A and B chains, calcitonin, beta endorphin, urogastrone, beta globin, myoglobin, human growth hormone, angiotensin, proline, proteases, beta-galactosidase, and cellulases.

The hybrid polypeptide, the SER region and the payload polypeptides may also include post-translational modifications known to the art such as glycosylation, acylation, and other modifications not interfering with the desired activity of the polypeptide.

Developing a Hybrid polypeptide

The SER region is present in genes involved in starch synthesis. Methods for isolating such genes include screening from genomic DNA libraries and from cDNA libraries. Genes can be cut and changed by ligation, mutation agents, digestion, restriction and other such procedures, e.g., as outlined in Maniatis et al., Molecular Cloning, Cold Spring Harbor Labs, Cold Spring Harbor, N.Y. Examples of excellent starting materials for accessing the PER region include, but are not limited to, the following: starch synthases I, II, III, IV, Branching Enzymes I, IIA and B and granule-bound starch synthase (GBSTS). These genes are present in starch-bearing plants such as rice, maize, peas, potatoes, wheat, and the like. Use of a probe of SER made from genomic DNA or cDNA or mRNA or antibodies raised against the SER allows for the isolation and identification of useful genes for cloning. The starch enzyme-encoding sequences may be modified as long as the modifications do not interfere with the ability of the SER region to encapsulate associated polypeptides.

When genes encoding proteins that are encapsulated into the starch granule are located, then several approaches to isolation of the SER can be employed, as is known to the art. One method is to cut the gene with restriction enzymes at various sites, deleting sections from the N-terminal end and allowing the resultant protein to express. The expressed truncated protein is then run on a starch gel to evaluate the association and dissociation constart of the remaining protein. Marker genes known to the art, e.g., green fluorescent protein gene, may be attached to the truncated protein and used to determine the presence of the marker gene in the starch granule.

Once the SPR gene sequence region is isolated it can be used in making the gene fragment sequence that will express the payload polypeptide encapsulated in starch. The SER gene sequence and the gene sequence encoding the payload polypeptide can be ligated together. The resulting fused DNA can then be placed in a number of vector constructs for expression in a number of hosts. The preferred hosts form starch granules in plastids, but the testing of the SER can be readily performed in bacterial hosts such as E. coli.

The nucleic acid sequence coding for the payload polypeptide may be derived from DNA, RNA, genomic DNA, cDNA, mRNA or may be synthesized in whole or in part. The sequence of the payload polypeptide can be manipulated to contain mutations such that the protein produced is a novel, mutant protein, so long as biological function is maintained.

When the payload polypeptide-encoding nucleic acid sequence is ligated onto the SER-encoding sequence, the gene sequence for the payload polypeptide is preferably attached at the end of the SER sequence coding for the N-terminus. Although the N-terminus end is preferred, it does not appear critical to the invention whether the payload polypeptide is ligated onto the N-terminus end or the C-terminus end of the SER. Clearly, the method of forming the recombinant nucleic acid molecules of this invention, whether synthetically, or by cloning and ligation, is not critical to the present invention.

The central region of the hybrid polypeptide is optional. For some applications of the present invention it can be very useful to introduce DNA coding for a convenient protease cleavage site in this region into the recombinant nucleic acid molecule used to express the hybrid polypeptide. Alternatively, it can be useful to introduce DNA coding for an amino acid sequence that is pH-sensitive to form the central region. If the use of the present invention is to develop a pure protein that can be extracted and released from the starch granule by a protease or the like, then a protease cleavage site is useful. Additionally, if the protein is to be digested in an animal then a protease cleavage site may be useful to assist the enzymes in the digestive tract of the animal to release the protein from the starch. In other applications and in many digestive uses the cleavage site would be superfluous.

The central region site may comprise a spacer. A spacer refers to a peptide that joins the proteins comprising a hybrid polypeptide. Usually it does not have any specific activity other than to join the proteins, to preserve some minimum distance, to influence the folding, charge or hydrophobic or hydrophilic nature of the hybrid polypeptide.

Construct Development

Once the ligated DNA which encodes the hybrid polypeptide is formed, then cloning vectors or plasmids are prepared which are capable of transferring the DNA to a host for expressing the hybrid polypeptides. The recombinant nucleic acid sequence of this invention is inserted into a convenient cloning vector or plasmid. For the present invention the preferred host is a starch granule-producing host. However, bacterial hosts can also be employed. Especially useful are bacterial hosts that have been transformed to contain some or all of the starch-synthesizing genes of a plant. The ordinarily skilled person in the art understands that the plasmid is tailored to the host. For example, in a bacterial host transcriptional regulatory promoters include lac, TAC, trp and the like. Additionally, DNA coding for a transit peptide most likely would not be used and a secretory leader that is upstream from the structural gene may be used to get the polypeptide into the medium. Alternatively, the product is retained in the host and the host is lysed and the product isolated and purified by starch extraction methods or by binding the material to a starch matrix (or a starch-like matrix such as amylose or amylopectin, glycogen or the like) to extract the product.

The preferred host is a plant and thus the preferred plasmid is adapted to be useful in a plant. The plasmid should contain a promoter, preferably a promoter adapted to target the expression of the protein in the starch-containing tissue of the plant. The promoter may be specific for various tissues such as seeds, roots, tubers and the like; or, it can be a constitutive promoter for gene expression throughout the tissues of the plant. Well-known promoters include the 10 kD zein (maize) promoter, the CAB promoter, patastin, 35S and 19S cauliflower mosaic virus promoters (very useful in dicots), the polyubiquitin promoter (useful in monocots) and enhancements and modifications thereof known to the art.

The cloning vector may contain coding sequences for a transit peptide to direct the plasmid into the correct location. Examples of transit peptide-coding sequences are shown in the sequence tables. Coding sequences for other transit peptides can be used. Transit peptides naturally occurring in the host to be used are preferred. Preferred transit peptide coding regions for maize are shown in the tables and figures hereof. The purpose of the transit peptide is to target the vector to the correct intracellular area.

Attached to the transit peptide-encoding sequence is the DNA sequence encoding the N-terminal end of the payload polypeptide. The direction of the sequence encoding the payload polypeptide is varied depending on whether sense or antisense transcription is desired. DNA constructs of this invention specifically described herein have the sequence encoding the payload polypeptide at the N-terminus end but the SER coding region can also be at the N-terminus end and the payload polypeptide sequence following. At the end of the DNA construct is the terminator sequence. Such sequences are well known in the art.

The cloning vector is transformed into a host. Introduction of the cloning vector, preferably a plasmid, into the host can be done by a number of transformation techniques known to the art. These techniques may vary by host but they include microparticle bombardment, micro injection, Agrobacterium transformation, "whiskers" technology (U.S. Pat. Nos. 5,302,523 and 5,464,765), electroporation and the like. If the host is a plant, the cells can be regenerated to form plants. Methods of regenerating plants are known in the art. Once the host is transformed and the proteins expressed therein, the presence of the DNA encoding the payload polypeptide in the host is confirmable. The presence of expressed proteins may be confirmed by Western Blot or ELISA or as a result of a change in the plant or the cell.

Uses of Encapsulated Protein

There are a number of applications of this invention. The hybrid polypeptide can be cleaved in a pure state from the starch (cleavage sites can be included) and pure protein can be recovered. Alternatively, the encapsulated payload polypeptide within the starch can be used in raw form to deliver protein to various parts of the digestive tract of the consuming animal ("animal" shall include mammals, birds and fish). For example if the starch in which the material is encapsulated is resistant to digestion then the protein will be released slowly into the intestine of the animal, therefore avoiding degradation of the valuable protein in the stomach. Amino acids such as methionine and lysine may be encapsulated to be incorporated directly into the grain that the animal is fed thus eliminating the need for supplementing the diet with these amino acids in other forms.

The present invention allows hormones, enzymes, proteins, proteinaceous nutrients and proteinaceous medicines to be targeted to specific digestive areas in the digestive tracts of animals. Proteins that normally are digested in the upper digestive tract encapsulated in starch are able to pass through the stomach in a nondigested manner and be absorbed intact cor in part by the intestine. If capable of passing through the intestinal wall, the payload polypeptides can be used for medicating an animal, or providing hormones such as growth factors, e.g., somatotropin, for vaccination of an animal or for enhancing the nutrients available to an animal.

If the starch used is not resistant to digestion in the stomach (for example the sugary 2 starch is highly digestible), then the added protein can be targeted to be absorbed in the upper digestive tract of the animal. This would require that the host used to produce the modified starch be mutated or transformed to make sugary 2 type starch. The present invention encompasses the use of mutant organisms that form modified starch as hosts. Some examples of these mutant hosts include rice and maize and the like having sugary 1, sugary 2, brittle, shrunken, waxy, amylose extender, dull, opaque, and floury mutations, and the like. These mutant starches and starches from different plant sources have different levels of digestibility. Thus by selection of the host for expression of the DNA and of the animal to which the modified starch is fed, the hybrid polypeptide can be digested where it is targeted. Different proteins are absorbed most efficiently by different parts of the body. By encapsulating the protein in starch that has the selected digestibility, the protein can be supplied anywhere throughout the digestive tract and at specific times during the digestive process.

Another of the advantages of the present invention is the ability to inhibit or express differing levels of glycosylation of the desired polypeptide. The encapsulating procedure may allow the protein to be expressed within the granule in a different glycosylation state than if expressed by other DNA molecules. The glycosylation will depend on the amount of encapsulation, the host employed and the sequence of the polypeptide.

Improved crops having the above-described characteristics may be produced by genetic manipulation of plants known to possess other favorable characteristics. By manipulating the nucleotide sequence of a starch-synthesizing enzyme gene, it is possible to alter the amount of key amino acids, proteins or peptides produced in a plant. One or more genetically engineered gene constructs, which may be of plant, fungal, bacterial or animal origin, may be incorporated into the plant genome by sexual crossing or by transformation. Engineered genes may comprise additional copies of wildtype genes or may encode modified or allelic or alternative enzymes with new properties. Incorporation of such gene construct(s) may have varying effects depending on the amount and type of gene(s) introduced (in a sense or antisense orientation). It may increase the plant's capacity to produce a specific protein, peptide or provide an improved amino acid balance.

Cloning Enzymes Involved in Starch Biosynthesis

Known cloning techniques may be used to provide the DNA constructs of this invention. The source of the special forms of the SSTS, GBSTS, BE, glycogen synthase (GS), amylopectin, or other genes used herein may be any organism that can make starch or glycogen. Potential donor organisms are screened and identified. Thereafter there can be two approaches (a) using enzyme purification and antibody/sequence generation following the protocols described herein; (b) using SSTS, GBSTS, BE, GS, amylopectin or other cDNAs as heterologous probes to identify the genomic DNAs for SSTS, GBSTS, BE, GS, amylopectin or other starch-encapsulating enzymes in libraries from the organism concerned. Gene transformation, plant regeneration and testing protocols are known to the art. In this instance it is necessary to make gene constructs for transformation which contain regulatory sequences that ensure expression during starch formation. These regulatory sequences are present in many small grains and in tubers and roots. For example these regulatory sequences are readily available in the maize endosperm in DNA encoding Granule Bound Starch Synthesis (GBSTS), Soluble Starch Synthases (SSTS) or Branching Enzymes (BE) or other maize endosperm starch synthesis pathway enzymes. These regulatory sequences from the endosperm ensure protein expression at the correct developmental time (e.g., ADPG pyrophosphorylase).

In this method we measure starch-binding constants of starch-binding proteins using native protein electrophoresis in the presence of suitable concentrations of carbohydrates such as glycogen or amylopectin. Starch-encapsulating regions can be elucidated using site-directed mutagenesis and other genetic engineering methods known to those skilled in the art. Novel genetically-engineered proteins carrying novel peptides or amino acid combinations can be evaluated using the methods described herein.

EXAMPLES Example One

Method for Identification of Starch-encapsulating Proteins

Starch-Granule Protein Isolation

Homogenize 12.5 g grain in 25 ml Extraction buffer (50 mM Tris acetate, pH 7.5, 1 mM EDTA, 1 mM DTT for 3×20 seconds in Waring blender with 1 min intervals between blending). Keep samples on ice. Filter through mira cloth and centrifuge at 6,000 rpm for 30 min. Discard supernatant and scrape off discolored solids which overlay white starch pellet. Resuspend pellet in 25 ml buffer and recentrifuge. Repeat washes twice more. Resuspend washed pellet in -20° C. acetone, allow pellet to settle at -20° C. Repeat. Dry starch under stream of air. Store at -20° C.

Protein Extraction

Mix 50 mg starch with 1 ml 2% SDS in eppendorf. Vortex, spin at 18,000 rpm, 5 min, 4° C. Pour off supernatant. Repeat twice. Add 1 ml sample buffer (4 ml distilled water, 1 ml 0.5 M Tris-HCl, pH 6.8, 0.8 ml glycerol, 1.6 ml 10% SDS, 0.4 ml B-mercaptoethanol, 0.2 ml 0.5% bromphenol blue). Boil eppendorf for 10 min with hole in lid. Cool, centrifuge 10,000 rpm for 10 min. Decant supernatant into new eppendorf. Boil for 4 minutes with standards. Cool.

SDS-Page Gels: (non-denaturing)

    ______________________________________                                                       10% Resolve                                                                              4% Stack                                               ______________________________________                                         Acryl/Bis 40% stock                                                                            2.5      ml       1.0  ml                                        1.5 M Tris pH 8.8 2.5 ml --                                                    0.5 M Tris pH 8.8 --  2.5 ml                                                   10% SDS 100 μl 100 μl                                                    Water 4.845 ml 6.34 ml                                                         Degas 15 min add fresh 50 μl 50 μl                                       10% Ammonium Persulfate                                                        TEMED 5 μl 10 μl                                                       ______________________________________                                    

Mini-Protean II Dual Slab Cell; 3.5 ml of Resolve buffer per gel. 4% Stack is poured on top. The gel is run at 200V constant voltage. 10× Running buffer (250 mM Tris, 1.92 M glycine, 1% SDS, pH 8.3).

Method of Measurement of Starch-Encapsulating Regions

Solutions

    ______________________________________                                         Extraction Buffer:                                                                             50 mM Tris-acetate pH 7.5, 10 mM                                  EDTA, 10%                                                                      sucrose, 2.5 mM DTT-fresh.                                                    Stacking Buffer: 0.5 M Tris-HCl, pH 6.8                                        Resolve Buffer: 1.5 M Tris-HCl, pH 8.8                                         10 X Lower Electrode Buffer: 30.3 g Tris + 144 g Glycine                        qs to 1 L. (pH is ˜8.3, no                                               adjustment). Dilute for use.                                                  Upper Electrode Buffer: Same as Lower                                          Sucrose Solution: 18.66 g sucrose + 100 ml                                      dH.sub.2 O                                                                    30% Acryl/Bis Stock (2.67% C): 146 g acrylamide + 4 g                           bis + 350 ml dH.sub.2 O. Bring up                                              to 500 ml. Filter and store at                                                 4 C in the dark for up                                                         to 1 month.                                                                   15% Acryl/Bis Stock (20% C): 6 g acrylamide + 1.5 g                             bis + 25 ml dH.sub.2 O. Bring up                                               to 50 ml. Filter and store at 4 C in                                           the dark for up to                                                             1 month.                                                                      Riboflavin Solution: 1.4 g riboflavin + 100 ml                                  dH.sub.2 O. Store in dark for up                                               to 1 month.                                                                   SS Assay mix: 25 mM Sodium Citrate, 25 mM                                       Bicine-NaOH (pH                                                                8.0), 2 mM EDTA, 1 mM DTT-fresh,                                               1 mM                                                                           Adenosine 5' Diphosphoglucose-                                                 fresh, 10 mg/ml rabbit                                                         liver glycogen Type III-fresh.                                                Iodine Solution: 2 g iodine + 20 g KI, 0.1 N HCl up                             to 1 L.                                                                     ______________________________________                                    

Extract

4 ml extraction buffer +12 g endosperm. Homogenize.

filter through mira cloth or 4 layers cheesecloth, spin 20,000 g (14,500 rpm, SM-24 rotor), 20 min., 4° C.

remove supernatant using a glass pipette.

0.85 ml extract +0.1 ml glycerol +0.05 ml 0.5% bromophenol blue.

vortex and spin 5 min. full speed microfuge. Use directly or freeze in liquid nitrogen and store at -80° C. for up to 2 weeks.

Cast Gels

Attach Gel Bond PAG film (FMC Industries, Rockland, Me.) to (inside of) outer glass plate using two-sided scotch tape, hydrophilic side up. The tape and the film is lined up as closely and evenly as possible with the bottom of the plate. The film is slightly smaller than the plate. Squirt water between the film and the plate to adhere the film. Use a tissue to push out excess water. Set up plates as usual, then seal the bottom of the plates with tacky adhesive. The cassette will fit into the casting stand if the gray rubber is removed from the casting stand. The gel polymerizes with the film, and stays attached during all subsequent manipulations.

Cast 4.5% T resolve mini-gel (0.75 mm):

2.25 ml dH₂ O

+3.75 ml sucrose solution

+2.5 ml resolve buffer

+1.5 ml 30% Acryl/Bis stock

+various amounts of glycogen for each gel (i.e., 0-1.0%)

DEGAS 15 MIN.

+50 μl 10% APS

+5 μl TEMED

POLYMERIZE FOR 30 MIN. OR OVERNIGHT

Cast 3.125% T stack:

1.59 ml dH₂ O

+3.75 ml sucrose solution

+2.5 ml stack buffer

+2.083 ml 15% Acryl/Bis stock

DO NOT DEGAS

15 μl 10% APS

+35 μl riboflavin solution

+30 μl TEMED

POLYMERIZE FOR 2.5 HOURS CLOSE TO A LIGHT BULB cool in 4° C. before pulling out combs. Can also not use combs, and just cast a centimeter of stacker.

The foregoing procedure:

Can run at different temperatures; preincubate gels and solutions.

Pre-run for 15 min. at 200 V

Load gel: 7 μl per well, or 115 μl if no comb.

Run at 140 V until dye front is close to bottom. Various running temperatures are achieved by placing the whole gel rig into a water bath. Can occasionally stop the run to insert a temperature probe into the gel.

Enzyme assay: Cut gels off at dye front. Incubate in SS. Assay mix overnight at room temperature with gentle shaking. Rinse gels with water. Flood with 12/KI solution.

Take pictures of the gels on a light box, and measure the pictures. Rm=mm from top of gel to the active band/mm from top of gel to the bottom of the gel where it was cut (whore the dye front was). Plot % glycogen vs. 1/Rm. The point where the line intersects the x axis is -K (where y=0).

Testing and evaluation protocol for SER region length

Following the procedure above for selection of the SER region requires four basic steps. First DNA encoding a protein having a starch-encapsulation region must be selected. This can )e selected from known starch-synthesizing genes or starch-binding genes such as genes for amylases, for example. The protein must be extracted. A number of protein extraction techniques are well known in the art. The protein may be treated with proteases to form protein fragments of different lengths. The preferred fragments have deletions primarily from the N-terminus region of the protein. The SER region is located nearer to the C-terminus end than the N-terminus end. The protein is run on the gels described above and affinity for the gel matrix is evaluated. Higher affinity shows more preference of that region of the protein for the matrix. This method enables comparison of different proteins to identify the starch-encapsulating regions in natural or synthetic proteins.

Example Two

SER Fusion Vecter

The following fusion vectors are adapted for use in E. coli. The fusion gene that was attached to the probable SER in these vectors encoded for the green fluorescent protein (GFP). Any number of different genes encoding for proteins and polypeptides could be ligated into the vectors. A fusion vector was constructed having the SER of waxy maize fused to a second gene or gene fragment, in this case GFP.

pEXS114 (see FIG. 1a): Synthetic GFP (SGFP) was PCR-amplified from the plasmid HBT-SGFP (from Jen Sheen; Dept. of Molecular Biology; Wellman 11, MGH; Boston, Mass. 02114) using the primers EXS73 (5'-GACTAGTCATATG GTG AGC AAG GGC GAG GAG-3') [SEQ ID NO:1] and EXS74 (5'-CTAGATCTTCATATG CTT GTA CAG CTC GTC CAT GCC-3') [SEQ ID NO:2]. The ends of the PCR product were polished off with T DNA polymerase to generate blunt ends; then the PCR product was digested with Spe I. This SGFP fragment was subcloned into the EcoRV-Spe I sites of pBSK (Stratagene it 11011 North Torrey Pines Rd. La Jolla, Calif.) to generate pEXS114.

pEXS 115 [see FIG. 1b]: Synthetic GFP (SGFP) was PCR-amplified from the plasmid HBT-SGFP (from Jen Sheen) using the primers EXS73 (see above) and EXS75 (5'-CTAGATCTTGGCCATGGC CTT GTA CAG CTC GTC CAT GCC-3') [SEQ ID NO:3]. The ends of the PCR product were polished off with T DNA polymerase to generate blunt ends; then the PCR product was digested with Spe I. This SGFP fragment was subcloned into the EcoRV-Spe I sites of pBSK (Stratagene) generating pEXS115.

pEXSWX (see FIG. 2a): Maize WX subcloned NdeI-Not I into pET-21a (see FIG. 2b). The genomic DNA sequence and associated amino acids from which the mRNA sequence can be generated is shown in TABLES 1a and 1b below and alternatively the DNA listed in the following tables could be employed.

                                      TABLE 1a                                     __________________________________________________________________________     DNA Sequence and Deduced Amino Acid Sequence                                     of the waxy Gene in Maize                                                      [SEQ ID NO:4 and SEQ ID NO:5]                                                __________________________________________________________________________     LOCUS      ZMWAXY       4800 bp    DNA             PLN                           DEFINITION Zea mays waxy (wx+) locus for UDP-glucose starch glycosyl                     transferase.                                                        ACCESSION  X03935 M24258                                                       KEYWORDS   glycosyl transferase; transit peptide;                                         UDP-glucose starch glycosyl transferase; waxy locus.                SOURCE     maize.                                                                ORGANISM Zea mays                                                                       Eukaryota; Plantae; Embryobionta; Magnoliophyta; Liliopsida;                   Commelinidae; Cyperales; Poaceae.                                   REFERENCE  1  (bases 1 to 4800)                                                  AUTHORS  Kloesgen,R.B., Gierl,A., Schwarz-Sommer,Z. and Saedler,H.             TITLE    Molecular analysis of the waxy locus of Zea mays                      JOURNAL  Mol. Gen. Genet. 203, 237-244 (1986)                                  STANDARD full automatic                                                      COMMENT    NCBI gi:  22509                                                     FEATURES             Location/Qualifiers                                            source          1..4800                                                                        /organism="Zea mays"                                           repeat_region   283..287                                                                       /note="direct repeat 1"                                        repeat_region   288..292                                                                       /note="direct repeat 1"                                        repeat_region   293..297                                                                       /note="direct repeat 1"                                        repeat_region   298..302                                                                       /note="direct repeat 1"                                        misc_feature    372..385                                                                       /note="GC stretch (pot. regulatory factor binding         site)"                                                                              misc_feature    442..468                                                                       /note="GC stretch (pot. regulatory factor binding         site)"                                                                              misc_feature    768..782                                                                       /note="GC stretch (pot. regulatory factor binding         site)"                                                                              misc_feature    810..822                                                                       /note="GC stretch (pot. regulatory factor binding         site)"                                                                              misc_feature    821..828                                                                       /note="target duplication site Ac7)"                           CAAT_signa1     821..828                                                       TATA_signal     867..873                                                       misc_feature    887..900                                                                       /note="GC stretch (pot. regulatory factor binding         site)"                                                                              misc_feature    901                                                                            /note=transcriptional start site"                              exon            901..1080                                                                      /number=1                                                      intron          1081..1219                                                                     /number= 1                                                     exon            1220..1553                                                                     /number=2                                                      transit_peptide 1233..1448                                                     CDS             join(1449..1553,1685..1765,1860..1958,2055..2144,         2226..2289,2413..2513,2651..2760,2858..3101,3212..3394,                                             3490..3681,3793..3879,3977..4105,4227..4343)                                   /note="NCBI gi:  22510"                                                        /codon start=1                                                                 /product="glucosyl transferase"                           /translation="ASAGMNVVFVGAEMAPWSKTGGLGDVLGGLPPAMAANGHRVMVV                      - SPRYDQYKDAWDTSWSEIKMGDGYETVRFFHCYKRGVDRVFVDHPLFLERVWGKTEE                    - KIYGPVAGTDYRDNQLRFSLLCQAALEAPRILSLNNNPYFSGPYGEDVVFVCNDWHTG                   - PLSCYLKSNYQSHGIYRDAKTAFCIHNISYQGRFAFSDYPELNLPERFKSSFDFIDGY                   - EKPVEGRKINWMKAGILEADRVLTVSPYYAEELISGIARGCELDNIMRLTGITGIVNG                   - MDVSEWDPSRDKYIAVKYDVSTAVEAKALNKEALQAEVGLPVDRNIPLVAFIGRLEEQ                   - KGPDVMAAAIPQLMEMVEDVQIVLLGTGKKKFERMLMSAEEKFPGKVRAVVKFNAALA                   - HHIMAGADVLAVTSRFEPCGLIQLQGMRYGTPCACASTGGLVDTIIEGKTGFHMGRLS                   - VDCNVVEPADVKKVATTLQRAIKVVGTPAYEEMVRNCMIQDLSWKGPAKNWENVLLSL                                       GVAGGEPGVEGEEIAPLAKENVAAP"                                     intron          1554..1684                                                                     /number=2                                                      exon            1685..1765                                                                     /number=3                                                      intron          1766..1859                                                                     /number=3                                                      exon            1860..1958                                                                     /number=4                                                      intron          1959..2054                                                                     /number=4                                                      exon            2055..2144                                                                     /number=S                                                      intron          2145..2225                                                                     /number= 5                                                     exon            2226..2289                                                                     /number=6                                                      intron          2290..2412                                                                     /number=6                                                      exon            2413..2513                                                                     /number=7                                                      intron          2514..2650                                                                     /number=7                                                      exon            2651..2760                                                                     /number=8                                                      intron          2761..2857                                                                     /number=8                                                      exon            2858..3101                                                                     /number=9                                                      intron          3102..3211                                                                     /number= 9                                                     exon            3212..3394                                                                     /number=10                                                     misc_feature    3358..3365                                                                     /note="target duplication site (Ac9)"                          intron          3395..3489                                                                     /number=10                                                     exon            3490..3681                                                                     /number=11                                                     misc_feature    3570..3572                                                                     /note="target duplication site (Sprn 18)"                      intron          3682..3792                                                                     /number=11                                                     exon            3793..3879                                                                     /number=12                                                     intron          3880..3976                                                                     /number=12                                                     exon            3977..4105                                                                     /number=13                                                     intron          4106..4226                                                                     /number=13                                                     exon            4227..4595                                                                     /number=14                                                     polyA_signal    4570..4575                                                     polyA_signal    4593..4598                                                     polyA_site      4595                                                           polyA_signal    4597..4602                                                     polyA_site      4618                                                           polyA_site      4625                                                      BASE COUNT      935 A   1413 C   1447 G   1005 T                               ORIGIN                                                                                 1 CAGCGACCTA TTACACAGCC CGCTCGGGCC CGCGACGTCG GGACACATCT              TCTTCCCCCT                                                                        -        61 TTTGGTGAAG CTCTGCTCGC AGCTGTCCGG CTCCTTGGAC GTTCGTGTGG          CAGATTCATC                                                                        -       121 TGTTGTCTCG TCTCCTGTGC TTCCTGGGTA GCTTGTGTAG TGGAGCTGAC          ATGGTCTGAG                                                                        -       181 CAGGCTTAAA ATTTGCTCGT AGACGAGGAG TACCAGCACA GCACGTTGCG          GATTTCTCTG                                                                        -       241 CCTGTGAAGT GCAACGTCTA GGATTGTCAC ACGCCTTGGT CGCGTCGCGT          CGCGTCGCGT                                                                        -       301 CGATGCGGTG GTGAGCAGAG CAGCAACAGC TGGGCGGCCC AACGTTGGCT          TCCGTGTCTT                                                                        -       361 CGTCGTACGT ACGCGCGCGC CGGGGACACG CAGCAGAGAG CGGAGAGCGA          GCCGTGCACG                                                                        -       421 GGGAGGTGGT GTGGAAGTGG AGCCGCGCGC CCGGCCGCCC GCGCCCGGTG          GGCAACCCAA                                                                        -       481 AAGTACCCAC GACAAGCGAA GGCGCCAAAG CGATCCAAGC TCCGGAACGC          AACAGCATGC                                                                        -       541 GTCGCGTCGG AGAGCCAGCC ACAAGCAGCC GAGAACCGAA CCGGTGGGCG          ACGCGTCATG                                                                        -       601 GGACGGACGC GGGCGACGCT TCCAAACGGG CCACGTACGC CGGCGTGTGC          GTGCGTGCAG                                                                        -       661 ACGACAAGCC AAGGCGAGGC AGCCCCCGAT CGGGAAAGCG TTTTGGGCGC          GAGCGCTGGC                                                                        -       721 GTGCGGGTCA GTCGCTGGTG CGCAGTGCCG GGGGGAACGG GTATCGTGGG          GGGCGCGGGC                                                                        -       781 GGAGGAGAGC GTGGCGAGGG CCGAGAGCAG CGCGCGGCCG GGTCACGCAA          CGCGCCCCAC                                                                        -       841 GTACTGCCCT CCCCCTCCGC GCGCGCTAGA AATACCGAGG CCTGGACCGG          GGGGGGGCCC                                                                        -       901 CGTCACATCC ATCCATCGAC CGATCGATCG CCACAGCCAA CACCACCCGC          CGAGGCGACG                                                                        -       961 CGACAGCCGC CAGGAGGAAG GAATAAACTC ACTGCCAGCC AGTGAAGGGG          GAGAAGTGTA                                                                        -      1021 CTGCTCCGTC GACCAGTGCG CGCACCGCCC GGCAGGGCTG CTCATCTCGT          CGACGACCAG                                                                        -      1081 GTTCTGTTCC GTTCCGATCC GATCCGATCC TGTCCTTGAG TTTCGTCCAG          ATCCTGGCGC                                                                        -      1141 GTATCTGCGT GTTTGATGAT CCAGGTTCTT CGAACCTAAA TCTGTCCGTG          CACACGTCTT                                                                        -      1201 TTCTCTCTCT CCTACGCAGT GGATTAATCG GCATGGCGGC TCTGGCCACG          TCGCAGCTCG                                                                        -      1261 TCGCAACGCG CGCCGGCCTG GGCGTCCCGG ACGCGTCCAC GTTCCGCCGC          GGCGCCGCGC                                                                        -      1321 AGGGCCTGAG GGGGGCCCGG GCGTCGGCGG CGGCGGACAC GCTCAGCATG          CGGACCAGCG                                                                        -      1381 CGCGCGCGGC GCCCAGGCAC CAGCAGCAGG CGCGCCGCGG GGGCAGGTTC          CCGTCGCTCG                                                                        -      1441 TCGTGTGCGC CAGCGCCGGC ATGAACGTCG TCTTCGTCGG CGCCGAGATG          GCGCCGTGGA                                                                        -      1501 GCAAGACCGG CGGCCTCGGC GACGTCCTCG GCGGCCTGCC GCCGGCCATG          GCCGTAAGCG                                                                        -      1561 CGCGCACCGA GACATGCATC CGTTGGATCG CGTCTTCTTC GTGCTCTTGC          CGCGTGCATG                                                                        -      1621 ATGCATGTGT TTCCTCCTGG CTTGTGTTCG TGTATGTGAC GTGTTTGTTC          GGGCATGCAT                                                                        -      1681 GCAGGCGAAC GGGCACCGTG TCATGGTCGT CTCTCCCCGC TACGACCAGT          ACAAGGACGC                                                                        -      1741 CTGGGACACC AGCGTCGTGT CCGAGGTACG GCCACCGAGA CCAGATTCAG          ATCACAGTCA                                                                        -      1801 CACACACCGT CATATGAACC TTTCTCTGCT CTGATGCCTG CAACTGCAAA          TGCATGCAGA                                                                        -      1861 TCAAGATGGG AGACGGGTAC GAGACGGTCA GGTTCTTCCA CTGCTACAAG          CGCGGAGTGG                                                                        -      1921 ACCGCGTGTT CGTTGACCAC CCACTGTTCC TGGAGAGGGT GAGACGAGAT          CTGATCACTC                                                                        -      1981 GATACGCAAT TACCACCCCA TTGTAAGCAG TTACAGTGAG CTTTTTTTCC          CCCCGGCCTG                                                                        -      2041 GTCGCTGGTT TCAGGTTTGG GGAAAGACCG AGGAGAAGAT CTACGGGCCT          GTCGCTGGAA                                                                        -      2101 CGGACTACAG GGACAACCAG CTGCGGTTCA GCCTGCTATG CCAGGTCAGG          ATGGCTTGGT                                                                        -      2161 ACTACAACTT CATATCATCT GTATGCAGCA GTATACACTG ATGAGAAATG          CATGCTGTTC                                                                        -      2221 TGCAGGCAGC ACTTGAAGCT CCAAGGATCC TGAGCCTCAA CAACAACCCA          TACTTCTCCG                                                                        -      2281 GACCATACGG TAAGAGTTGC AGTCTTCGTA TATATATCTG TTGAGCTCGA          GAATCTTCAC                                                                        -      2341 AGGAAGCGGC CCATCAGACG GACTGTCATT TTACACTGAC TACTGCTGCT          GCTCTTCGTC                                                                        -      2401 CATCCATACA AGGGGAGGAC GTCGTGTTCG TCTGCAACGA CTGGCACACC          GGCCCTCTCT                                                                        -      2461 CGTGCTACCT CAAGAGCAAC TACCAGTCCC ACGGCATCTA CAGGGACGCA          AAGGTTGCCT                                                                        -      2521 TCTCTGAACT GAACAACGCC GTTTTCGTTC TCCATGCTCG TATATACCTC          GTCTGGTAGT                                                                        -      2581 GGTGGTGCTT CTCTGAGAAA CTAACTGAAA CTGACTGCAT GTCTGTCTGA          CCATCTTCAC                                                                        -      2641 GTACTACCAG ACCGCTTTCT GCATCCACAA CATCTCCTAC CAGGGCCGGT          TCGCCTTCTC                                                                        -      2701 CGACTACCCG GAGCTGAACC TCCCGGAGAG ATTCAAGTCG TCCTTCGATT          TCATCGACGG                                                                        -      2761 GTCTGTTTTC CTGCGTGCAT GTGAACATTC ATGAATGGTA ACCCACAACT          GTTCGCGTCC                                                                        -      2821 TGCTGGTTCA TTATCTGACC TGATTGCATT ATTGCAGCTA CGAGAAGCCC          GTGGAAGGCC                                                                        -      2881 GGAAGATCAA CTGGATGAAG GCCGGGATCC TCGAGGCCGA CAGGGTCCTC          ACCGTCAGCC                                                                        -      2941 CCTACTACGC CGAGGAGCTC ATCTCCGGCA TCGCCAGGGG CTGCGAGCTC          GACAACATCA                                                                        -      3001 TGCGCCTCAC CGGCATCACC GGCATCGTCA ACGGCATGGA CGTCAGCGAG          TGGGACCCCA                                                                        -      3061 GCAGGGACAA GTACATCGCC GTGAAGTACG ACGTGTCGAC GGTGAGCTGG          CTAGCTCTGA                                                                        -      3121 TTCTGCTGCC TGGTCCTCCT GCTCATCATG CTGGTTCGGT ACTGACGCGG          CAAGTGTACG                                                                        -      3181 TACGTGCGTG CGACGGTGGT GTCCGGTTCA GGCCGTGGAG GCCAAGGCGC          TGAACAAGGA                                                                        -      3241 GGCGCTGCAG GCGGAGGTCG GGCTCCCGGT GGACCGGAAC ATCCCGCTGG          TGGCGTTCAT                                                                        -      3301 CGGCAGGCTG GAAGAGCAGA AGGGCCCCGA CGTCATGGCG GCCGCCATCC          CGCAGCTCAT                                                                        -      3361 GGAGATGGTG GAGGACGTGC AGATCGTTCT GCTGGTACGT GTGCGCCGGC          CGCCACCCGG                                                                        -      3421 CTACTACATG CGTGTATCGT TCGTTCTACT GGAACATGCG TGTGAGCAAC          GCGATGGATA                                                                        -      3481 ATGCTGCAGG GCACGGGCAA GAAGAAGTTC GAGCGCATGC TCATGAGCGC          CGAGGAGAAG                                                                        -      3541 TTCCCAGGCA AGGTGCGCGC CGTGGTCAAG TTCAACGCGG CGCTGGCGCA          CCACATCATG                                                                        -      3601 GCCGGCGCCG ACGTGCTCGC CGTCACCAGC CGCTTCGAGC CCTGCGGCCT          CATCCAGCTG                                                                        -      3661 CAGGGGATGC GATACGGAAC GGTACGAGAG AAAAAAAAAA TCCTGAATCC          TGACGAGAGG                                                                        -      3721 GACAGAGACA GATTATGAAT GCTTCATCGA TTTGAATTGA TTGATCGATG          TCTCCCGCTG                                                                        -      3781 CGACTCTTGC AGCCCTGCGC CTGCGCGTCC ACCGGTGGAC TCGTCGACAC          CATCATCGAA                                                                        -      3841 GGCAAGACCG GGTTCCACAT GGGCCGCCTC AGCGTCGACG TAAGCCTAGC          TCTGCCATGT                                                                        -      3901 TCTTTCTTCT TTCTTTCTGT ATGTATGTAT GAATCAGCAC CGCCGTTCTT          GTTTCGTCGT                                                                        -      3961 CGTCCTCTCT TCCCAGTGTA ACGTCGTGGA GCCGGCGGAC GTCAAGAAGG          TGGCCACCAC                                                                        -      4021 ATTGCAGCGC GCCATCAAGG TGGTCGGCAC GCCGGCGTAC GAGGAGATGG          TGAGGAACTG                                                                        -      4081 CATGATCCAG GATCTCTCCT GGAAGGTACG TACGCCCGCC CCGCCCCGCC          CCGCCAGAGC                                                                        -      4141 AGAGCGCCAA GATCGACCGA TCGACCGACC ACACGTACGC GCCTCGCTCC          TGTCGCTGAC                                                                        -      4201 CGTGGTTTAA TTTGCGAAAT GCGCAGGGCC CTGCCAAGAA CTGGGAGAAC          GTGCTGCTCA                                                                        -      4261 GCCTCGGGGT CGCCGGCGGC GAGCCAGGGG TCGAAGGCGA GGAGATCGCG          CCGCTCGCCA                                                                        -      4321 AGGAGAACGT GGCCGCGCCC TGAAGAGTTC GGCCTGCAGG GCCCCTGATC          TCGCGCGTGG                                                                        -      4381 TGCAAAGATG TTGGGACATC TTCTTATATA TGCTGTTTCG TTTATGTGAT          ATGGACAAGT                                                                        -      4441 ATGTGTAGCT GCTTGCTTGT GCTAGTGTAA TGTAGTGTAG TGGTGGCCAG          TGGCACAACC                                                                        -      4501 TAATAAGCGC ATGAACTAAT TGCTTGCGTG TGTAGTTAAG TACCGATCGG          TAATTTTATA                                                                        -      4561 TTGCGAGTAA ATAAATGGAC CTGTAGTGGT GGAGTAAATA ATCCCTGCTG          TTCGGTGTTC                                                                        -      4621 TATCGCTCC TCGTATAGAT ATTATATAGA GTACATTTTT CTCTCTCTGA           ATCCTACGTT                                                                        -      4681 TGTGAAATTT CTATATCATT ACTGTAAAAT TTCTGCGTTC CAAAAGAGAC          CATAGCCTAT                                                                        -      4741 CTTTGGCCCT GTTTGTTTCG GCTTCTGGCA GCTTCTGGCC ACCAAAAGCT          GCTGCGGACT                                                                     __________________________________________________________________________

                                      TABLE lb                                     __________________________________________________________________________     DNA Sequence and Deduced Amino Acid Sequence in waxy Gene in Rice               [SEQ ID NO:6 and SEQ ID NO:7]                                                 __________________________________________________________________________     LOCUS      OSWX         2542 bp    RNA             PLN                          DEFINITION O.sativa Waxy mRNA.                                                 ACCESSION  X62134 S39554                                                       KEYWORDS   glucosyltransferase; starch biosynthesis; waxy gene.                SOURCE     rice.                                                                 ORGANISM Oryza sativa                                                                   Eukaryota; Plantae; Embryobionta; Magnoliophyta; Liliopsida;                   Commelinidae; Cyperales; Poaceae.                                   REFERENCE  1  (bases 1 to 2542)                                                  AUTHORS  Okayaki, R.J.                                                         TITLE    Direct Submission                                                     JOURNAL  Submitted (12-SEP-1991) to the EMBL/GenBank/DDBJ databases.         R.J.                                                                                      Okayaki, University of Florida, Dep of Vegetable Crops, 1255                   Fifield Hall, 514 IFAS, Gainesville, Florida 32611-0514, USA          STANDARD full automatic                                                      REFERENCE  2 (bases 1 to 2542)                                                   AUTHORS  Okagaki,R.J.                                                          TITLE    Nucleotide sequence of a long cDNA from the rice waxy gene            JOURNAL  Plant Mol. Biol. 19, 513-516 (1992)                                   STANDARD full automatic                                                      COMMENT    NCBI gi:  20402                                                     FEATURES             Location/Qualifiers                                            source          1..2542                                                                        /organism="Oryza sativa"                                                       /dev_stage="immature seed"                                                     /tissue type="seed"                                            CDS             453..2282                                                                      /gene="Wx"                                                                     /standard_name="Waxy gene"                                                     /EC_number="2.4.1.21"                                                          /note="NCBI gi:  20403"                                                        /codon_start=1                                                                 /function="starch biosynthesis"                                                /product="starch (bacterial glycogen) synthase"           /translation="MSALTTSQLATSATGFGIADRSAPSSLLRHGFQGLKPRSPAGGD                      - ATSLSVTTSARATPKQQRSVQRGSRRFPSVVVYATGAGMNVVFVGAEMAPWSKTGGLG                   - DVLGGLPPAMAANGHRVMVISPRYDQYKDAWDTSVVAEIKVADRYERVRFFHCYKRGV                   - DRVFIDHPSFLEKVWGKTGEKIYGPDTGVDYKDNQMRFSLLCQAALEAPRILNLNNNP                   - YFKGTYGEDVVFVCNDWHTGPLASYLKNNYQPNGIYRNAKVAFCIHNISYQGRFAFED                   - YPELNLSERFRSSFDFIDGYDTPVEGRKINWMKAGILEADRVLTVSPYYAEELISGIA                   - RGCELDNIMRLTGITGIVNGMDVSEWDPSKDKYITAKYDATTAIEAKALNKEALQAEA                   - GLPVDRKIPLIAFIGRLEEQKGPDVMAAAIPELMQEDVQIVLLGTGKKKFEKLLKSME                   - EKYPGKVRAVVKFNAPLAHLIMAGADVLAVPSRFEPCGLIQLQGMRYGTPCACASTGG                   - LVDTVIEGKTGFHMGRLSVDCKVVEPSDVKKVAATLKRAIKVVGTPAYEEMVRNCMNQ                                       DLSWKGPAKNWENVLLGLGVAGSAPGIEGDEIAPLAKENVAAP"                   3'UTR           2283..2535                                                     polyA_site      2535                                                      BASE COUNT      610 A    665 C    693 G    574 T                               ORIGIN                                                                                 1 GAATTCAGTG TGAAGGAATA GATTCTCTTC AAAACAATTT AATCATTCAT              CTGATCTGCT                                                                        -        61 CAAAGCTCTG TGCATCTCCG GGTGCAACGG CCAGGATATT TATTGTGCAG          TAAAAAAATG                                                                        -       121 TCATATCCCC TAGCCACCCA AGAAACTGCT CCTTAAGTCC TTATAAGCAC          ATATGGCATT                                                                        -       181 GTAATATATA TGTTTGAGTT TTAGCGACAA TTTTTTTAAA AACTTTTGGT          CCTTTTTATG                                                                        -       241 AACGTTTTAA GTTTCACTGT CTTTTTTTTT CGAATTTTAA ATGTAGCTTC          AAATTCTAAT                                                                        -       301 CCCCAATCCA AATTGTAATA AACTTCAATT CTCCTAATTA ACATCTTAAT          TCATTTATTT                                                                        -       361 GAAAACCAGT TCAAATTCTT TTTAGGCTCA CCAAACCTTA AACAATTCAA          TTCAGTGCAG                                                                        -       421 AGATCTTCCA CAGCAACAGC TAGACAACCA CCATGTCGGC TCTCACCACG          TCCCAGCTCG                                                                        -       481 CCACCTCGGC CACCGGCTTC GGCATCGCCG ACAGGTCGGC GCCGTCGTCG          CTGCTCCGCC                                                                        -       541 ACGGGTTCCA GGGCCTCAAG CCCCGCAGCC CCGCCGGCGG CGACGCGACG          TCGCTCAGCG                                                                        -       601 TGACGACCAG CGCGCGCGCG ACGCCCAAGC AGCAGCGGTC GGTGCAGCGT          GGCAGCCGGA                                                                        -       661 GGTTCCCCTC CGTCGTCGTG TACGCCACCG GCGCCGGCAT GAACGTCGTG          TTCGTCGGCG                                                                        -       721 CCGAGATGGC CCCCTGGAGC AAGACCGGCG GCCTCGGTGA CGTCCTCGGT          GGCCTCCCCC                                                                        -       781 CTGCCATGGC TGCGAATGGC CACAGGGTCA TGGTGATCTC TCCTCGGTAC          GACCAGTACA                                                                        -       841 AGGACGCTTG GGATACCAGC GTTGTGGCTG AGATCAAGGT TGCAGACAGG          TACGAGAGGG                                                                        -       901 TGAGGTTTTT CCATTGCTAC AAGCGTGGAG TCGACCGTGT GTTCATCGAC          CATCCGTCAT                                                                        -       961 TCCTGGAGAA GGTTTGGGGA AAGACCGGTG AGAAGATCTA CGGACCTGAC          ACTGGAGTTG                                                                        -      1021 ATTACAAAGA CAACCAGATG CGTTTCAGCC TTCTTTGCCA GGCAGCACTC          GAGGCTCCTA                                                                        -      1081 GGATCCTAAA CCTCAACAAC AACCCATACT TCAAAGGAAC TTATGGTGAG          GATGTTGTGT                                                                        -      1141 TCGTCTGCAA CGACTGGCAC ACTGGCCCAC TGGCGAGCTA CCTGAAGAAC          AACTACCAGC                                                                        -      1201 CCAATGGCAT CTACAGGAAT GCAAAGGTTG CTTTCTGCAT CCACAACATC          TCCTACCAGG                                                                        -      1261 GCCGTTTCGC TTTCGAGGAT TACCCTGAGC TGAACCTCTC CGAGAGGTTC          AGGTCATCCT                                                                        -      1321 TCGATTTCAT CGACGGGTAT GACACGCCGG TGGAGGGCAG GAAGATCAAC          TGGATGAAGG                                                                        -      1381 CCGGAATCCT GGAAGCCGAC AGGGTGCTCA CCGTGAGCCC GTACTACGCC          GAGGAGCTCA                                                                        -      1441 TCTCCGGCAT CGCCAGGGGA TGCGAGCTCG ACAACATCAT GCGGCTCACC          GGCATCACCG                                                                        -      1501 GCATCGTCAA CGGCATGGAC GTCAGCGAGT GGGATCCTAG CAAGGACAAG          TACATCACCG                                                                        -      1561 CCAAGTACGA CGCAACCACG GCAATCGAGG CGAAGGCGCT GAACAAGGAG          GCGTTGCAGG                                                                        -      1621 CGGAGGCGGG TCTTCCGGTC GACAGGAAAA TCCCACTGAT CGCGTTCATC          GGCAGGCTGG                                                                        -      1681 AGGAACAGAA GGGCCCTGAC GTCATGGCCG CCGCCATCCC GGAGCTCATG          CAGGAGGACG                                                                        -      1741 TCCAGATCGT TCTTCTGGGT ACTGGAAAGA AGAAGTTCGA GAAGCTGCTC          AAGAGCATGG                                                                        -      1801 AGGAGAAGTA TCCGGGCAAG GTGAGGGCGG TGGTGAAGTT CAACGCGCCG          CTTGCTCATC                                                                        -      1861 TCATCATGGC CGGAGCCGAC GTGCTCGCCG TCCCCAGCCG CTTCGAGCCC          TGTGGACTCA                                                                        -      1921 TCCAGCTGCA GGGGATGAGA TACGGAACGC CCTGTGCTTG CGCGTCCACC          GGTGGGCTCG                                                                        -      1981 TGGACACGGT CATCGAAGGC AAGACTGGTT TCCACATGGG CCGTCTCAGC          GTCGACTGCA                                                                        -      2041 AGGTGGTGGA GCCAAGCGAC GTGAAGAAGG TGGCGGCCAC CCTGAAGCGC          GCCATCAAGG                                                                        -      2101 TCGTCGGCAC GCCGGCGTAC GAGGAGATGG TCAGGAACTG CATGAACCAG          GACCTCTCCT                                                                        -      2161 GGAAGGGGCC TGCGAAGAAC TGGGAGAATG TGCTCCTGGG CCTGGGCGTC          GCCGGCAGCG                                                                        -      2221 CGCCGGGGAT CGAAGGCGAC GAGATCGCGC CGCTCGCCAA GGAGAACGTG          GCTGCTCCTT                                                                        -      2281 GAAGAGCCTG AGATCTACAT ATGGAGTGAT TAATTAATAT AGCAGTATAT          GGATGAGAGA                                                                        -      2341 CGAATGAACC AGTGGTTTGT TTGTTGTAGT GAATTTGTAG CTATAGCCAA          TTATATAGGC                                                                        -      2401 TAATAAGTTT GATGTTGTAC TCTTCTGGGT GTGCTTAAGT ATCTTATCGG          ACCCTGAATT                                                                        -      2461 TATGTGTGTG GCTTATTGCC AATAATATTA AGTAATAAAG GGTTTATTAT          ATTATTATAT                                                                        -      2521 ATGTTATATT ATACTAAAAA AA                                        __________________________________________________________________________

                                      TABLE 2                                      __________________________________________________________________________     DNA Sequence and Deduced Amino Acid Sequence of                                  the Soluble Starch Synthase IIa Gene in Maize                                  [SEQ ID NO:8 and SEQ  ID NO:9]                                               __________________________________________________________________________        FILE  NAME: MSS2C.SEQ    SEQUENCE:NORMAL    2007 BP                            - CODON TABLE: UNIV.TCN                                                        - SEQUENCE    REGION:     1-   2007                                            - TRANSLATION REGION: 1-   2007                                                - *** DNA TRANSLATION ***                                                      - 1 GCT GAG GCT GAG GCC GGG GGC AAG GAC GCG CCG CCG GAG AGG AGC GGC 48        1  A   E   A   E   A   G   G   K   D   A   P   P   E   R   S   G  16                                                     - 49 GAC GCC GCC AGG TTG CCC                                                 CGC GCT CGG CGC AAT GCG GTC TCC                                                AAA CGG 96                             17  D   A   A   R   L   P   R   A   R   R   N   A   V   S   K   R 32                                                     - 97 AGG GAT CCT CTT CAG CCG                                                 GTC GGC CGG TAC GGC TCC GCG ACG                                                GGA AAC 144                            33  R   D   P   L   Q   P   V   G   R   Y   G   S   A   T   G   N  48                                                    - 145 ACG GCC AGG ACC GGC GCC                                                GCG TCC TGC CAG AAC GCC GCA TTG                                                GCG GAC 192                             49  T   A   R   T   G   A   A   S   C   Q   N   A   A   L   A   D  64                                                   - 193 GTT GAG ATC GTT GAG ATC                                                AAG TCC ATC GTC GCC GCG CCG CCG                                                ACG AGC 240                             65  V   E   I   V   E   I   K   S   I   V   A   A   P   P   T   S 80                                                    - 241 ATA GTG AAG TTC CCA GGG                                                CGC GGG CTA CAG GAT GAT CCT TCC                                                CTC TGG 288                             81  I   V   K   F   P   G   R   G   L   Q   D   D   P   S   L   W  96                                                   - 289 GAC ATA GCA CCG GAG ACT                                                GTC CTC CCA GCC CCG AAG CCA CTG                                                CAT GAA 336                             97  D   I   A   P   E   T   V   L   P   A   P   K   P   L   H   E 112                                                   - 337 TCG CCT GCG GTT GAC GGA                                                GAT TCA AAT GGA ATT GCA CCT CCT                                                ACA GTT 384                            113  S   P   A   V   D   G   D   S   N   G   I   A   P   P   T   V 128                                                   - 385 GAG CCA TTA GTA CAG GAG                                                GCC ACT TGG GAT TTC AAG AAA TAC                                                ATC GGT 432                            129  E   P   L   V   Q   E   A   T   W   D   F   K   K   Y   I   G 144                                                   - 433 TTT GAC GAG CCT GAC GAA                                                GCG AAG GAT GAT TCC AGG GTT GGT                                                GCA GAT 480                            145  F   D   E   P   D   E   A   K   D   D   S   R   V   G   A   D 160                                                   - 481 GAT GCT GGT TCT TTT GAA                                                CAT TAT GGG ACA ATG ATT CTG GGC                                                CTT TGT 528                            161  D   A   G   S   F   E   H   Y   G   T   M   I   L   G   L   C 176                                                   - 529 GGG GAG AAT GTT ATG AAC                                                GTG ATC GTG GTG GCT GCT GAA TGT                                                TCT CCA 576                            177  G   E   N   V   M   N   V   I   V   V   A   A   E   C   S   P 192                                                   - 577 TGG TGC AAA ACA GGT GGT                                                CTT GGA GAT GTT GTG GGA GCT TTA                                                CCC AAG 624                            193  W   C   K   T   G   G   L   G   D   V   V   G   A   L   P   K 208                                                   - 625 GCT TTA GCG AGA AGA GGA                                                CAT CGT GTT ATG GTT GTG GTA CCA                                                AGG TAT 672                            209  A   L   A   R   R   G   H   R   V   M   V   V   V   P   R   Y 224                                                   - 673 GGG GAC TAT GTG GAA GCC                                                TTT GAT ATG GGA ATC CGG AAA TAC                                                TAC AAA 720                            225  G   D   Y   V   E   A   F   D   M   G   I   R   K   Y   Y   K 240                                                   - 721 GCT GCA GGA CAG GAC CTA                                                GAA GTG AAC TAT TTC CAT GCA TTT                                                ATT GAT 768                            241  A   A   G   Q   D   L   E   V   N   Y   F   H   A   F   I   D 256                                                   - 769 GGA GTC GAC TTT GTG TTC                                                ATT GAT GCC TCT TTC CGG CAC CGT                                                CAA GAT 816                            257  G   V   D   F   V   F   I   D   A   S   F   R   H   R   Q   D 272                                                   - 817 GAC ATA TAT GGG GGA AGT                                                AGG CAG GAA ATC ATG AAG CGC ATG                                                ATT TTG 864                            273  D   I   Y   G   G   S   R   Q   E   I   M   K   R   M   I   L 288                                                   - 865 TTT TGC AAG GTT GCT GTT                                                GAG GTT CCT TGG CAC GTT CCA TGC                                                GGT GGT 912                            289  F   C   K   V   A   V   E   V   P   W   H   V   P   C   G   G 304                                                   - 913 GTG TGC TAC GGA GAT GGA                                                AAT TTG GTG TTC ATT GCC ATG AAT                                                TGG CAC 960                            305  V   C   Y   G   D   G   N   L   V   F   I   A   M   N   W   H 320                                                   - 961 ACT GCA CTC CTG CCT GTT                                                TAT CTG AAG GCA TAT TAC AGA GAC                                                CAT GGG 1008                           321  T   A   L   L   P   V   Y   L   K   A   Y   Y   R   D   H   G  336         - 1009 TTA ATG CAG TAC ACT CGC TCC GTC CTC GTC ATA CAT AAC ATC GGC CAC                                                1056                                    337   L   M   Q   Y   T   R   S   V   L   V   I   H   N   I   G   H                                                   352                                     - 1057 CAG GGC CGT GGT CCT GTA CAT GAA TTC CCG TAC ATG GAC TTG CTG AAC                                                1104                                    353   Q   G   R   G   P   V   H   E   F   P   Y   M   D   L   L   N                                                   368                                     - 1105 ACT AAC CTT CAA CAT TTC GAG CTG TAC GAT CCC GTC GGT GGC GAG CAC                                                1152                                    369   T   N   L   Q   H   F   E   L   Y   D   P   V   G   G   E   H                                                   384                                     - 1153 GCC AAC ATC TTT GCC GCG TGT GTT CTG AAG ATG GCA GAC CGG GTG GTG                                                1200                                    385   A   N   I   F   A   A   C   V   L   K   M   A   D   R   V   V                                                   400                                     - 1201 ACT GTC AGC CGC GGC TAC CTG TGG GAG CTG AAG ACA GTG GAA GGC GGC                                                1248                                    401   T   V   S   R   G   Y   L   W   E   L   K   T   V   E   G   G                                                   416                                     - 1249 TGG GGC CTC CAC GAC ATC ATC CGT TCT AAC GAC TGG AAG ATC AAT GGC                                                1296                                    417   W   G   L   H   D   I   I   R   S   N   D   W   K   I   N   G                                                   432                                     - 1297 ATT CGT GAA CGC ATC GAC CAC CAG GAG TGG AAC CCC AAG GTG GAC GTG                                                1344                                    433   I   R   E   R   I   D   H   Q   E   W   N   P   K   V   D   V                                                   448                                     - 1345 CAC CTG CGG TCG GAC GGC TAC ACC AAC TAC TCC CTC GAG ACA CTC GAC                                                1392                                    449   H   L   R   S   D   G   Y   T   N   Y   S   L   E   T   L   D                                                   464                                     - 1393 GCT GGA AAG CGG CAG TGC AAG GCG GCC CTG CAG CGG GAC GTG GGC CTG                                                1440                                    465   A   G   K   R   Q   C   K   A   A   L   Q   R   D   V   G   L                                                   480                                     - 1441 GAA GTG CGC GAC GAC GTG CCG CTG CTC GGC TTC ATC GGG CGT CTG GAT                                                1488                                    481   E   V   R   D   D   V   P   L   L   G   F   I   G   R   L   D                                                   496                                     - 1489 GGA CAG AAG GGC GTG GAC ATC ATC GGG GAC GCG ATG CCG TGG ATC GCG                                                1536                                    497   G   Q   K   G   V   D   I   I   G   D   A   M   P   W   I   A                                                   512                                     - 1537 GGG CAG GAC GTG CAG CTG GTG ATG CTG GGC ACC GGC CCA CCT GAC CTG                                                1584                                    513  G   Q   D   V   Q   L   V   M   L   G   T   G   P   P   D   L                                                    528                                     - 1585 GAA CGA ATG CTG CAG CAC TTG GAG CGG GAG CAT CCC AAC AAG GTG CGC                                                1632                                    529  E   R   M   L   Q   H   L   E   R   E   H   P   N   K   V   R                                                    544                                     - 1633 GGG TGG GTC GGG TTC TCG GTC CTA ATG GTG CAT CGC ATC ACG CCG GGC                                                1680                                    545  G   W   V   G   F   S   V   L   M   V   H   R   I   T   P   G                                                    560                                     - 1681 GCC AGC GTG CTG GTG ATG CCC TCC CGC TTC GCC GGC GGG CTG AAC CAG                                                1728                                    561  A   S   V   L   V   M   P   S   R   F   A   G   G   L   N   Q                                                    576                                     - 1729 CTC TAC GCG ATG GCA TAC GGC ACC GTC CCT GTG GTG CAC GCC GTG GGC                                                1776                                    577  L   Y   A   M   A   Y   G   T   V   P   V   V   H   A   V   G                                                    592                                     - 1777 GGG CTC AGG GAC ACC GTG GCG CCG TTC GAC CCG TTC GGC GAC GCC GGG                                                1824                                    593  G   L   R   D   T   V   A   P   F   D   P   F   G   D   A   G                                                    608                                     - 1825 CTC GGG TGG ACT TTT GAC CGC GCC GAG GCC AAC AAG CTG ATC GAG GTG                                                1872                                    609  L   G   W   T   F   D   R   A   E   A   N   K   L   I   E   V                                                    624                                     - 1873 CTC AGC CAC TGC CTC GAC ACG TAC CGA AAC TAC GAG GAG AGC TGG AAG                                                1920                                    625  L   S   H   C   L   D   T   Y   R   N   Y   E   E   S   W   K                                                    640                                     - 1921 AGT CTC CAG GCG CGC GGC ATG TCG CAG AAC CTC AGC TGG GAC CAC GCG                                                1968                                    641  S   L   Q   A   R   G   M   S   Q   N   L   S   W   D   H   A                                                    656                                     - 1969 GCT GAG CTC TAC GAG GAC GTC CTT GTC AAG TAC CAG TGG 2007                                                         657  A   E   L   Y   E   D                                                   V   L   V   K   Y   Q   W            __________________________________________________________________________                                               669                             

                                      TABLE 3                                      __________________________________________________________________________     DNA Sequence and Deduced Amino Acid Sequence of                                  The Soluble Starch Synthase IIb Gene in Maize                                  [SEQ ID NO:10 and SEQ ID NO:11]                                              __________________________________________________________________________        FILE  NAME:  MSS3FULL.DNA    SEQUENCE:  NORMAL   2097 BP                       - CODON TABLE:  UNIV.TCN                                                       - SEQUENCE    REGION:      1-   2097                                           - TRANSLATION REGION:      1-   2097                                           - *** DNA TRANSLATION ***                                                      - 1 ATG CCG GGG GCA ATC TCT TCC TCG TCG TCG GCT TTT CTC CTC CCC GTC 48        1  M   P   G   A   I   S   S   S   S   S   A   F   L   L   P   V 16                                                      - 49 GCG TCC TCC TCG CCG CGG                                                 CGC AGG CGG GGC AGT GTG GGT GCT                                                GCT CTG 96                             17  A   S   S   S   P   R   R   R   R   G   S   V   G   A   A   L 32                                                     - 97 CGC TCG TAC GGC TAC AGC                                                 GGC GCG GAG CTG CGG TTG CAT TGG                                                GCG CGG 144                            33  R   S   Y   G   Y   S   G   A   E   L   R   L   H   W   A   R  48                                                    - 145 CGG GGC CCG CCT CAG GAT                                                GGA GCG GCG TCG GTA CGC GCC GCA                                                GCG GCA 192                             49  R   G   P   P   Q   D   G   A   A   S   V   R   A   A   A   A  64                                                   - 193 CCG GCC GGG GGC GAA AGC                                                GAG GAG GCA GCG AAG AGC TCC TCC                                                TCG TCC 240                             65  P   A   G   G   E   S   E   E   A   A   K   S   S   S   S   S  80                                                   - 241 CAG GCG GGC GCT GTT CAG                                                GGC AGC ACG GCC AAG GCT GTG GAT                                                TCT GCT 288                             81  Q   A   G   A   V   Q   G   S   T   A   K   A   V   D   S   A  96                                                   - 289 TCA CCT CCC AAT CCT TTG                                                ACA TCT GCT CCG AAG CAA AGT CAG                                                AGC GCT 336                             97  S   P   P   N   P   L   T   S   A   P   K   Q   S   Q   S   A 112                                                   - 337 GCA ATG CAA AAC GGA ACG                                                AGT GGG GGC AGC AGC GCG AGC ACC                                                GCC GCG 384                            113  A   M   Q   N   G   T   S   G   G   S   S   A   S   T   A   A 128                                                   - 385 CCG GTG TCC GGA CCC AAA                                                GCT GAT CAT CCA TCA GCT CCT GTC                                                ACC AAG 432                            129  P   V   S   G   P   K   A   D   H   P   S   A   P   V   T   K 144                                                   - 433 AGA GAA ATC GAT GCC AGT                                                GCG GTG AAG CCA GAG CCC GCA GGT                                                GAT GAT 480                            145  R   E   I   D   A   S   A   V   K   P   E   P   A   G   D   D 160                                                   - 481 GCT AGA CCG GTG GAA AGC                                                ATA GGC ATC GCT GAA CCG GTG GAT                                                GCT AAG 528                            161  A   R   P   V   E   S   I   G   I   A   E   P   V   D   A   K 176                                                   - 529 GCT GAT GCA GCT CCG GCT                                                ACA GAT GCG GCG GCG AGT GCT CCT                                                TAT GAC 576                            177  A   D   A   A   P   A   T   D   A   A   A   S   A   P   Y   D 192                                                   - 577 AGG GAG GAT AAT GAA CCT                                                GGC CCT TTG GCT GGG CCT AAT GTG                                                ATG AAC 624                            193  R   E   D   N   E   P   G   P   L   A   G   P   N   V   M   N 208                                                   - 625 GTC GTC GTG GTG GCT TCT                                                GAA TGT GCT CCT TTC TGC AAG ACA                                                GGT GGC 672                            209  V   V   V   V   A   S   E   C   A   P   F   C   K   T   G   G 224                                                   - 673 CTT GGA GAT GTC GTG GGT                                                GCT TTG CCT AAG GCT CTG GCG AGG                                                AGA GGA 720                            225  L   G   D   V   V   G   A   L   P   K   A   L   A   R   R   G 240                                                   - 721 CAC CGT GTT ATG GTC GTG                                                ATA CCA AGA TAT GGA GAG TAT GCC                                                GAA GCC 768                            241  H   R   V   M   V   V   I   P   R   Y   G   E   Y   A   E   A 256                                                   - 769 CGG GAT TTA GGT GTA AGG                                                AGA CGT TAC AAG GTA GCT GGA CAG                                                GAT TCA 816                            257  R   D   L   G   V   R   R   R   Y   K   V   A   G   Q   D   S 272                                                   - 817 GAA GTT ACT TAT TTT CAC                                                TCT TAC ATT GAT GGA GTT GAT TTT                                                GTA TTC 864                            273  E   V   T   Y   F   H   S   Y   I   D   G   V   D   F   V   F 288                                                   - 865 GTA GAA GCC CCT CCC TTC                                                CGG CAC CGG CAC AAT AAT ATT TAT                                                GGG GGA 912                            289  V   E   A   P   P   F   R   H   R   H   N   N   I   Y   G   G 304                                                   - 913 GAA AGA TTG GAT ATT TTG                                                AAG CGC ATG ATT TTG TTC TGC AAG                                                GCC GCT 960                            305  E   R   L   D   I   L   K   R   M   I   L   F   C   K   A   A 320                                                   - 961 GTT GAG GTT CCA TGG TAT                                                GCT CCA TGT GGC GGT ACT GTC TAT                                                GGT GAT 1008                           321  V   E   V   P   W   Y   A   P   C   G   G   T   V   Y   G   D  336         - 1009 GGC AAC TTA GTT TTC ATT GCT AAT GAT TGG CAT ACC GCA CTT CTG CCT                                                1056                                    337  G   N   L   V   F   I   A   N   D   W   H   T   A   L   L   P                                                    352                                     - 1057 GTC TAT CTA AAG GCC TAT TAC CGG GAC AAT GGT TTG ATG CAG TAT GCT                                                1104                                    353  V   Y   L   K   A   Y   Y   R   D   N   G   L   M   Q   Y   A                                                    368                                     - 1105 CGC TCT GTG CTT GTG ATA CAC AAC ATT GCT CAT CAG GGT CGT GGC CCT                                                1152                                    369  R   S   V   L   V   I   H   N   I   A   H   Q   G   R   G   P                                                    384                                     - 1153 GTA GAC GAC TTC GTC AAT TTT GAC TTG CCT GAA CAC TAC ATC GAC CAC                                                1200                                    385  V   D   D   F   V   N   F   D   L   P   E   H   Y   I   D   H                                                    400                                     - 1201 TTC AAA CTG TAT GAC AAC ATT GGT GGG GAT CAC AGC AAC GTT TTT GCT                                                1248                                    401  F   K   L   Y   D   N   I   G   G   D   H   S   N   V   F   A                                                    416                                     - 1249 GCG GGG CTG AAG ACG GCA GAC CGG GTG GTG ACC GTT AGC AAT GGC TAC                                                1296                                    417  A   G   L   K   T   A   D   R   V   V   T   V   S   N   G   Y                                                    432                                     - 1297 ATG TGG GAG CTG AAG ACT TCG GAA GGC GGG TGG GGC CTC CAC GAC ATC                                                1344                                    433  M   W   E   L   K   T   S   E   G   G   W   G   L   H   D   I                                                    448                                     - 1345 ATA AAC CAG AAC GAC TGG AAG CTG CAG GGC ATC GTG AAC GGC ATC GAC                                                1392                                    449  I   N   Q   N   D   W   K   L   Q   G   I   V   N   G   I   D                                                    464                                     - 1393 ATG AGC GAG TGG AAC CCC GCT GTG GAC GTG CAC CTC CAC TCC GAC GAC                                                1440                                    465  M   S   E   W   N   P   A   V   D   V   H   L   H   S   D   D                                                    480                                     - 1441 TAC ACC AAC TAC ACG TTC GAG ACG CTG GAC ACC GGC AAG CGG CAG TGC                                                1488                                    481  Y   T   N   Y   T   F   E   T   L   D   T   G   K   R   Q   C                                                    496                                     - 1489 AAG GCC GCC CTG CAG CGG CAG CTG GGC CTG CAG GTC CGC GAC GAC GTG                                                1536                                    497  K   A   A   L   Q   R   Q   L   G   L   Q   V   R   D   D   V                                                    512                                     - 1537 CCA CTG ATC GGG TTC ATC GGG CGG CTG GAC CAC CAG AAG GGC GTG GAC                                                1584                                   513  P   L   I   G   F   I   G   R   L   D   H   Q   K   G   V   D 528                                                   - 1585 ATC ATC GCC GAC GCG                                                   ATC CAC TGG ATC GCG GGG CAG GAC                                                GTG CAG CTC 632                        529  I   I   A   D   A   I   H   W   I   A   G   Q   D   V   Q   L 544                                                   - 1633 GTG ATG CTG GGC ACC                                                   GGG CGG GCC GAC CTG GAG GAC ATG                                                CTG CGG CGG 1680                       545  V   M   L   G   T   G   R   A   D   L   E   D   M   L   R   R 560                                                   - 1681 TTC GAG TCG GAG CAC                                                   AGC GAC AAG GTG CGC GCG TGG GTG                                                GGG TTC TCG 1728                       561  F   E   S   E   H   S   D   K   V   R   A   W   V   G   F   S 576                                                   - 1729 GTG CCC CTG GCG CAC                                                   CGC ATC ACG GCG GGC GCG GAC ATC                                                CTG CTG ATG 1776                       577  V   P   L   A   H   R   I   T   A   G   A   D   I   L   L   M 592                                                   - 1777 CCG TCG CGG TTC GAG                                                   CCG TGC GGG CTG AAC CAG CTC TAC                                                GCC ATG GCG 1824                       593  P   S   R   F   E   P   C   G   L   N   Q   L   Y   A   M   A 608                                                   - 1825 TAC GGG ACC GTG CCC                                                   GTG GTG CAC GCC GTG GGG GGG CTC                                                CGG GAC ACG 1872                       609  Y   G   T   V   P   V   V   H   A   V   G   G   L   R   D   T 624                                                   - 1873 GTG GCG CCG TTC GAC                                                   CCG TTC AAC GAC ACC GGG CTC GGG                                                TGG ACG TTC 1920                       625  V   A   P   F   D   P   F   N   D   T   G   L   G   W   T   F 640                                                   - 1921 GAC CGC GCG GAG GCG                                                   AAC CGG ATG ATC GAC GCG CTC TCG                                                CAC TGC CTC 1968                       641  D   R   A   E   A   N   R   M   I   D   A   L   S   H   C   L 656                                                   - 1969 ACC ACG TAC CGG AAC                                                   TAC AAG GAG AGC TGG CGC GCC TGC                                                AGG GCG CGC 2016                       657  T   T   Y   R   N   Y   K   E   S   W   R   A   C   R   A   R 672                                                   - 2017 GGC ATG GCC GAG GAC                                                   CTC AGC TGG GAC CAC GCC GCC GTG                                                CTG TAT GAG 2064                       673  G   M   A   E   D   L   S   W   D   H   A   A   V   L   Y   E 688                                                   - 2065 GAC GTG CTC GTC AAG                                                   GCG AAG TAC CAG TGG TGA 2097                                                    689  D   V   L   V   K   A   K                                                  Y   Q   W   * 699                  __________________________________________________________________________

                                      TABLE 4                                      __________________________________________________________________________     DNA and Deduced Amino Acid Sequence of                                           The Soluble Starch Synthase I Gene in Maize                                    [SEQ ID NO:12; SEQ ID NO:13]                                                 __________________________________________________________________________     FILE  NAME : MSS1FULL.DNA    SEQUENCE:NORMAL   1752 BP                            - CODON TABLE: UNIV.TCN                                                        - SEQUENCE   REGION:      1-1752                                               - TRANSLATION REGION:      1-1752                                              - TGC GTC GCG GAG CTG AGC AGG GAG GGG CCC GCG CCG CGC CCG CTG CCA 48                                                Cys Val Ala Glu Leu Ser Arg Glu                                               Gly Pro Ala Pro Arg Pro Leu Pro                                                 700                 705                                                             710                 715                                                    - CCC GCG CTG CTG GCG CCC CCG                                                CTC GTG CCC GGC TTC CTC GCG CCG                                                CCG 96                                    Pro Ala Leu Leu Ala Pro Pro Leu Val Pro Gly Phe Leu Ala Pro Pro                                                                      720                                                             725                 730                  - GCC GAG CCC ACG GGT GAG CCG GCA TCG ACG CCG CCG CCC GTG CCC GAC 144                                               Ala Glu Pro Thr Gly Glu Pro Ala                                               Ser Thr Pro Pro Pro Val Pro Asp                                                             735                                                               740                 745                    - GCC GGC CTG GGG GAC CTC GGT CTC GAA CCT GAA GGG ATT GCT GAA GGT 192                                               Ala Gly Leu Gly Asp Leu Gly Leu                                               Glu Pro Glu Gly Ile Ala Glu Gly                                                        750                 755                                                             760                           - TCC ATC GAT AAC ACA GTA GTT GTG GCA AGT GAG CAA GAT TCT GAG ATT 240                                               Ser Ile Asp Asn Thr Val Val Val                                               Ala Ser Glu Gln Asp Ser Glu Ile                                                     765                 770                                                             775                              - GTG GTT GGA AAG GAG CAA GCT CGA GCT AAA GTA ACA CAA AGC ATT GTC 288                                               Val Val Gly Lys Glu Gln Ala Arg                                               Ala Lys Val Thr Gln Ser Ile Val                                                 780                 785                                                             790                 795                                                    - TTT GTA ACC GGC GAA GCT TCT                                                CCT TAT GCA AAG TCT GGG GGT CTA                                                GGA 336                                   Phe Val Thr Gly Glu Ala Ser Pro Tyr Ala Lys Ser Gly Gly Leu Gly                                                      800                 805                                                             810                                  - GAT GTT TGT GGT TCA TTG CCA GTT GCT CTT GCT GCT CGT GGT CAC CGT 384                                               Asp Val Cys Gly Ser Leu Pro Val                                               Ala Leu Ala Ala Arg Gly His Arg                                                              815                                                              820                 825                    - GTG ATG GTT GTA ATG CCC AGA TAT TTA AAT GGT ACC TCC GAT AAG AAT 432                                               Val Met Val Val Met Pro Arg Tyr                                               Leu Asn Gly Thr Ser Asp Lys Asn                                                         830                 835                                                             840                          - TAT GCA AAT GCA TTT TAC ACA GAA AAA CAC ATT CGG ATT CCA TGC TTT 480                                               Tyr Ala Asn Ala Phe Tyr Thr Glu                                               Lys His Ile Arg Ile Pro Cys Phe                                                     845                 850                                                             855                              - GGC GGT GAA CAT GAA GTT ACC TTC TTC CAT GAG TAT AGA GAT TCA GTT 528                                               Gly Gly Glu His Glu Val Thr Phe                                               Phe His Glu Tyr Arg Asp Ser Val                                                 860                 865                                                             870                 875                                                    - GAC TGG GTG TTT GTT GAT CAT                                                CCC TCA TAT CAC AGA CCT GGA AAT                                                TTA 576                                   Asp Trp Val Phe Val Asp His Pro Ser Tyr His Arg Pro Gly Asn Leu                                                                      880                                                             885                 890                  - TAT GGA GAT AAG TTT GGT GCT TTT GGT GAT AAT CAG TTC AGA TAC ACA 624                                               Tyr Gly Asp Lys Phe Gly Ala Phe                                               Gly Asp Asn Gln Phe Arg Tyr Thr                                                             895                                                               900                 905                    - CTC CTT TGC TAT GCT GCA TGT GAG GCT CCT TTG ATC CTT GAA TTG GGA 672                                               Leu Leu Cys Tyr Ala Ala Cys Glu                                               Ala Pro Leu Ile Leu Glu Leu Gly                                                        910                 915                                                             920                           - GGA TAT ATT TAT GGA CAG AAT TGC ATG TTT GTT GTC AAT GAT TGG CAT 720                                               Gly Tyr Ile Tyr Gly Gln Asn Cys                                               Met Phe Val Val Asn Asp Trp His                                                     925                 930                                                             935                              - GCC AGT CTA GTG CCA GTC CTT CTT GCT GCA AAA TAT AGA CCA TAT GGT 768                                               Ala Ser Leu Val Pro Val Leu Leu                                               Ala Ala Lys Tyr Arg Pro Tyr Gly                                                 940                 945                                                             950                 955                                                    - GTT TAT AAA GAC TCC CGC AGC                                                ATT CTT GTA ATA CAT AAT TTA GCA                                                CAT 816                                   Val Tyr Lys Asp Ser Arg Ser Ile Leu Val Ile His Asn Leu Ala His                                                                      960                                                             965                 970                  - CAG GGT GTA GAG CCT GCA AGC ACA TAT CCT GAC CTT GGG TTG CCA CCT 864                                               Gln Gly Val Glu Pro Ala Ser Thr                                               Tyr Pro Asp Leu Gly Leu Pro Pro                                                             975                                                               980                 985                    - GAA TGG TAT GGA GCT CTG GAG TGG GTA TTC CCT GAA TGG GCG AGG AGG 912                                               Glu Trp Tyr Gly Ala Leu Glu Trp                                               Val Phe Pro Glu Trp Ala Arg Arg                                                         990                 995                                                             1000                         - CAT GCC CTT GAC AAG GGT GAG GCA GTT AAT TTT TTG AAA GGT GCA GTT 960                                               His Ala Leu Asp Lys Gly Glu Ala                                               Val Asn Phe Leu Lys Gly Ala Val                                                     1005                1010                                                            1015                             - GTG ACA GCA GAT CGA ATC GTG ACT GTC AGT AAG GGT TAT TCG TGG GAG 1008        Val Thr Ala Asp Arg Ile Val Thr Val Ser Lys Gly Tyr Ser Trp Glu                                                      1020                1025                                                            1030                1035                                                   - GTC ACA ACT GCT GAA GGT GGA                                                CAG GGC CTC AAT GAG CTC TTA AGC                                                TCC 1056                                  Val Thr Thr Ala Glu Gly Gly Gln Gly Leu Asn Glu Leu Leu Ser Ser                                                                      1040                                                            1045                1050                                                       - AGA AAG AGT GTA TTA AAC GGA                                                ATT GTA AAT GGA ATT GAC ATT AAT                                                GAT 1104                                  Arg Lys Ser Val Leu Asn Gly Ile Val Asn Gly Ile Asp Ile Asn Asp                                                                  1055                                                              1060                1065                   - TGG AAC CCT GCC ACA GAC AAA TGT ATC CCC TGT CAT TAT TCT GTT GAT 1152        Trp Asn Pro Ala Thr Asp Lys Cys Ile Pro Cys His Tyr Ser Val Asp                                                              1070                1075                                                            1080                         - GAC CTC TCT GGA AAG GCC AAA TGT AAA GGT GCA TTG CAG AAG GAG CTG 1200        Asp Leu Ser Gly Lys Ala Lys Cys Lys Gly Ala Leu Gln Lys Glu Leu                                                          1085                1090                                                            1095                             - GGT TTA CCT ATA AGG CCT GAT GTT CCT CTG ATT GGC TTT ATT GGA AGG 1248        Gly Leu Pro Ile Arg Pro Asp Val Pro Leu Ile Gly Phe Ile Gly Arg                                                      1100                1105                                                            1110                1115                                                   - TTG GAT TAT CAG AAA GGC ATT                                                GAT CTC ATT CAA CTT ATC ATA CCA                                                GAT 1296                                  Leu Asp Tyr Gln Lys Gly Ile Asp Leu Ile Gln Leu Ile Ile Pro Asp                                                                      1120                                                            1125                1130                                                       - CTC ATG CGG GAA GAT GTT CAA                                                TTT GTC ATG CTT GGA TCT GGT GAC                                                CCA 1344                                  Leu Met Arg Glu Asp Val Gln Phe Val Met Leu Gly Ser Gly Asp Pro                                                                  1135                                                              1140                1145                   - GAG CTT GAA GAT TGG ATG AGA TCT ACA GAG TCG ATC TTC AAG GAT AAA 1392        Glu Leu Glu Asp Trp Met Arg Ser Thr Glu Ser Ile Phe Lys Asp Lys                                                              1150                1155                                                            1160                         - TTT CGT GGA TGG GTT GGA TTT AGT GTT CCA GTT TCC CAC CGA ATA ACT 1440        Phe Arg Gly Trp Val Gly Phe Ser Val Pro Val Ser His Arg Ile Thr                                                          1165                1170                                                            1175                             - GCC GGC TGC GAT ATA TTG TTA ATG CCA TCC AGA TTC GAA CCT TGT GGT 1488        Ala Gly Cys Asp Ile Leu Leu Met Pro Ser Arg Phe Glu Pro Cys Gly                                                      1180                1185                                                            1190                1195                                                   - CTC AAT CAG CTA TAT GCT ATG                                                CAG TAT GGC ACA GTT CCT GTT GTC                                                CAT 1536                                  Leu Asn Gln Leu Tyr Ala Met Gln Tyr Gly Thr Val Pro Val Val His                                                                      1200                                                            1205                1210                                                       - GCA ACT GGG GGC CTT AGA GAT                                                ACC GTG GAG AAC TTC AAC CCT TTC                                                GGT 1584                                  Ala Thr Gly Gly Leu Arg Asp Thr Val Glu Asn Phe Asn Pro Phe Gly                                                                  1215                                                              1220                1225                   - GAG AAT GGA GAG CAG GGT ACA GGG TGG GCA TTC GCA CCC CTA ACC ACA 1632        Glu Asn Gly Glu Gln Gly Thr Gly Trp Ala Phe Ala Pro Leu Thr Thr                                                              1230                1235                                                            1240                         - GAA AAC ATG TTT GTG GAC ATT GCG AAC TGC AAT ATC TAC ATA CAG GGA 1680        Glu Asn Met Phe Val Asp Ile Ala Asn Cys Asn Ile Tyr Ile Gln Gly                                                          1245                1250                                                            1255                             - ACA CAA GTC CTC CTG GGA AGG GCT AAT GAA GCG AGG CAT GTC AAA AGA 1728        Thr Gln Val Leu Leu Gly Arg Ala Asn Glu Ala Arg His Val Lys Arg                                                      1260                1265                                                            1270                1275                                                   - CTT CAC GTG GGA CCA TGC CGC                                                TGA 1752                                  Leu His Val Gly Pro Cys Arg  *                                                                 1280                                                            - (2) INFORMATION FOR SEQ ID NO:13:                                            -        (i) SEQUENCE CHARACTERISTICS:                                                     (A) LENGTH: 584 amino acids                                                    (B) TYPE: amino acid                                                           (D) TOPOLGGY: linear                                               -       (ii) MOLECULE TYPE: protein                                            -       (xi) SEQUENCE DESCRIPTION: SEQ ID NO:13:                               - Cys Val Ala Glu Leu Ser Arg Glu Gly Pro Ala Pro Arg Pro Leu Pro                                                     1               5                                                              15                                      - Pro Ala Leu Leu Ala Pro Pro Leu Val Pro Gly Phe Leu Ala Pro Pro                                                                20                                                               25                  30                     - Ala Glu Pro Thr Gly Glu Pro Ala Ser Thr Pro Pro Pro Val Pro Asp                                                            35                  40                                                              45                          - Ala Gly Leu Gly Asp Leu Gly Leu Glu Pro Glu Gly Ile Ala Glu Gly                                                       50                  55                                                              60                               - Ser Ile Asp Asn Thr Val Val Val Ala Ser Glu Gln Asp Ser Glu Ile                                                    65                  70                                                              75                  80                                                    - Val Val Gly Lys Glu Gln Ala                                                Arg Ala Lys Val Thr Gln Ser Ile                                                Val                                                        85                  90                  95                     - Phe Val Thr Gly Glu Ala Ser Pro Tyr Ala Lys Ser Gly Gly Leu Gly                                                               100                                                               105                 110                    - Asp Val Cys Gly Ser Leu Pro Val Ala Leu Ala Ala Arg Gly His Arg                                                           115                 120                                                             125                          - Val Met Val Val Met Pro Arg Tyr Leu Asn Gly Thr Ser Asp Lys Asn                                                       130                 135                                                             140                              - Tyr Ala Asn Ala Phe Tyr Thr Glu Lys His Ile Arg Ile Pro Cys Phe                                                   145                 150                                                             155                 160                                                    - Gly Gly Glu His Glu Val Thr                                                Phe Phe His Glu Tyr Arg Asp Ser                                                Val                                                       165                 170                 175                     - Asp Trp Val Phe Val Asp His Pro Ser Tyr His Arg Pro Gly Asn Leu                                                               180                                                               185                 190                    - Tyr Gly Asp Lys Phe Gly Ala Phe Gly Asp Asn Gln Phe Arg Tyr Thr                                                           195                 200                                                             205                          - Leu Leu Cys Tyr Ala Ala Cys Glu Ala Pro Leu Ile Leu Glu Leu Gly                                                       210                 215                                                             220                              - Gly Tyr Ile Tyr Gly Gln Asn Cys Met Phe Val Val Asn Asp Trp His                                                   225                 230                                                             235                 240                                                    - Ala Ser Leu Val Pro Val Leu                                                Leu Ala Ala Lys Tyr Arg Pro Tyr                                                Gly                                                       245                 250                 255                     - Val Tyr Lys Asp Ser Arg Ser Ile Leu Val Ile His Asn Leu Ala His                                                               260                                                               265                 270                    - Gln Gly Val Glu Pro Ala Ser Thr Tyr Pro Asp Leu Gly Leu Pro Pro                                                           275                 280                                                             285                          - Glu Trp Tyr Gly Ala Leu Glu Trp Val Phe Pro Glu Trp Ala Arg Arg                                                       290                 295                                                             300                              - His Ala Leu Asp Lys Gly Glu Ala Val Asn Phe Leu Lys Gly Ala Val                                                   305                 310                                                             315                 320                                                    - Val Thr Ala Asp Arg Ile Val                                                Thr Val Ser Lys Gly Tyr Ser Trp                                                Glu                                                       325                 330                 335                     - Val Thr Thr Ala Glu Gly Gly Gln Gly Leu Asn Glu Leu Leu Ser Ser                                                               340                                                               345                 350                    - Arg Lys Ser Val Leu Asn Gly Ile Val Asn Gly Ile Asp Ile Asn Asp                                                           355                 360                                                             365                          - Trp Asn Pro Ala Thr Asp Lys Cys Ile Pro Cys His Tyr Ser Val Asp                                                       370                 375                                                             380                              - Asp Leu Ser Gly Lys Ala Lys Cys Lys Gly Ala Leu Gln Lys Glu Leu                                                   385                 390                                                             395                 400                                                    - Gly Leu Pro Ile Arg Pro Asp                                                Val Pro Leu Ile Gly Phe Ile Gly                                                Arg                                                       405                 410                 415                     - Leu Asp Tyr Gln Lys Gly Ile Asp Leu Ile Gln Leu Ile Ile Pro Asp                                                               420                                                               425                 430                    - Leu Met Arg Glu Asp Val Gln Phe Val Met Leu Gly Ser Gly Asp Pro                                                           435                 440                                                             445                          - Glu Leu Glu Asp Trp Met Arg Ser Thr Glu Ser Ile Phe Lys Asp Lys                                                       450                 455                                                             460                              - Phe Arg Gly Trp Val Gly Phe Ser Val Pro Val Ser His Arg Ile Thr                                                   465                 470                                                             475                 480                                                    - Ala Gly Cys Asp Ile Leu Leu                                                Met Pro Ser Arg Phe Glu Pro Cys                                                Gly                                                       485                 490                 495                     - Leu Asn Gln Leu Tyr Ala Met Gln Tyr Gly Thr Val Pro Val Val His                                                               500                                                               505                 510                    - Ala Thr Gly Gly Leu Arg Asp Thr Val Glu Asn Phe Asn Pro Phe Gly                                                          515                 520                                                             525                           - Glu Asn Gly Glu Gln Gly Thr Gly Trp Ala Phe Ala Pro Leu Thr Thr                                                       530                 535                                                             540                              - Glu Asn Met Phe Val Asp Ile Ala Asn Cys Asn Ile Tyr Ile Gln Gly                                                   545                 550                                                             555                 560                                                    - Thr Gln Val Leu Leu Gly Arg                                                Ala Asn Glu Ala Arg His Val Lys                                                Arg                                                       565                 570                 575                     - Leu His Val Gly Pro Cys Arg  *                                                          580                                                              __________________________________________________________________________

                                      TABLE 5                                      __________________________________________________________________________     mRNA Sequence and Deduced Amino Acid Sequence of                                 The Maize Branching Enzyme II Gene and the Transit Peptide                     [SEQ ID NO:14 and SEQ ID NO:15]                                              __________________________________________________________________________     LOCUS          MZEGLUCTRN   2725 bp ss-mRNA            PLN                       DEFINITION     Corn starch branching enzyme II mRNA, complete cds.            ACCESSION      L08065                                                          KEYWORDS       1,4-alpha-glucan branching enzyme; amylo-transglycosylase;                     glucanotransferase; starch branching enzyme II.                 SOURCE         Zea mays cDNA to mRNA.                                            ORGANISM     Zea mays                                                                       Eukaryota; Plantae; Embryobionta; Magnoliophyta; Liliopsid     a;                                                                                             Commelinidae; Cyperales; Poaceae.                               REFERENCE      1  (bases 1 to 2725)                                              AUTHORS      Fisher,D. K., Boyer,C. D. and Hannah,L. C.                        TITLE        Starch branching enzyme II from maize endosperm                   JOURNAL      Plant Physiol. 102, 1045-1046 (1993)                              STANDARD     full automatic                                                  COMMENT        NCBI gi: 168482                                                 FEATURES                Location/Qualifiers                                         source             1..2725                                                                        /cultivar="W64Ax182E"                                                          /dev.sub.-- stage="29 days post pollenation"                                   /tissue.sub.-- type="endosperm"                                                /organism="Zea mays"                                        sig.sub.-- peptide        91..264                                                                 /codon.sub.-- start=1                                       CDS                91..2490                                                                       /EC.sub.-- number="2.4.1.18"                                                   /note="NCBI gi: 168483"                                                        /codon.sub.-- start=1                                                          /product="starch branching enzyme II"                   - /translation="MAFRVSGAVLGGAVRAPRLTGGGEGSLVFRHTGLFLTRGARVGC                   - SGTHGAMRAAAAARKAVMVPEGENDGLASRADSAQFQSDELEVPDISEETTCGAGVAD                   - AQALNRVRVVPPPSDGQKIFQIDPMLQGYKYHLEYRYSLYRRIRSDIDEHEGGLEAFS                   - RSYEKFGFNASAEGITYREWAPGAFSAALVGDVNNWDPNADRMSKNEFGVWEIFLPNN                   - ADGTSPIPHGSRVKVRMDTPSGIKDSIPAWIKYSVQAPGEIPYDGIYYDPPEEVKYVF                   - RHAQPKRPKSLRIYETHVGMSSPEPKINTYVNFRDEVLPRIKKLGYNAVQIMAIQEHS                   - YYGSFGYHVTNFFAPSSRFGTPEDLKSLIDRAHELGLLVLMDVVHSHASSNTLDGLNG                   - FDGTDTHYFHSGPRGHHWMWDSRLFNYGNWEVLRFLLSNARWWLEEYKFDGFRFDGVT                   - SMMYTHHGLQVTFTGNFNEYFGFATDVDAVVYLMLVNDLIHGLYPEAVTIGEDVSGMP                   - TFALPVHDGGVGFDYRMHMAVADKWIDLLKQSDETWKMGDIVHTLTNRRWLEKCVTYA                   - ESHDQALVGDKTIAFWLMDKDMYDFMALDRPSTPTIDRGIALHKMIRLITMGLGGEGY                   - LNFMGNEFGHPEWIDFPRGPQRLPSGKFIPGNNNSYDKCRRRFDLGDADYLRYHGMQE                   - FDQAMQHLEQKYEFMTSDHQYISRKHEEDKVIVFEKGDLVFVFNFHCNNSYFDYRIGC                   - RKPGVYKVVLDSDAGLFGGFSRIHHAAEHFTADCSHDNRPYSFSVYTPSRTCVVYAPV                   -                      E"                                                          mat.sub.-- peptide     265..2487                                                               /codon.sub.-- start=1                                                          /product="starch branching enzyme II"                     BASE COUNT      727 A    534 C    715 G    749 T                               ORIGIN                                                                              1 GGCCCAGAGC AGACCCGGAT TTCGCTCTTG CGGTCGCTGG GGTTTTAGCA TTGGCTGATC        -     61 AGTTCGATCC GATCCGGCTG CGAAGGCGAG ATGGCGTTCC GGGTTTCTGG              GGCGGTGCTC                                                                        -    121 GGTGGGGCCG TAAGGGCTCC CCGACTCACC GGCGGCGGGG AGGGTAGTCT             AGTCTTCCGG                                                                        -    181 CACACCGGCC TCTTCTTAAC TCGGGGTGCT CGAGTTGGAT GTTCGGGGAC             GCACGGGGCC                                                                        -    241 ATGCGCGCGG CGGCCGCGGC CAGGAAGGCG GTCATGGTTC CTGAGGGCGA             GAATGATGGC                                                                        -    301 CTCGCATCAA GGGCTGACTC GGCTCAATTC CAGTCGGATG AACTGGAGGT             ACCAGACATT                                                                        -    361 TCTGAAGAGA CAACGTGCGG TGCTGGTGTG GCTGATGCTC AAGCCTTGAA             CAGAGTTCGA                                                                        -    421 GTGGTCCCCC CACCAAGCGA TGGACAAAAA ATATTCCAGA TTGACCCCAT             GTTGCAAGGC                                                                        -    481 TATAAGTACC ATCTTGAGTA TCGGTACAGC CTCTATAGAA GAATCCGTTC             AGACATTGAT                                                                        -    541 GAACATGAAG GAGGCTTGGA AGCCTTCTCC CGTAGTTATG AGAAGTTTGG             ATTTAATGCC                                                                        -    601 AGCGCGGAAG GTATCACATA TCGAGAATGG GCTCCTGGAG CATTTTCTGC             AGCATTGGTG                                                                        -    661 GGTGACGTCA ACAACTGGGA TCCAAATGCA GATCGTATGA GCAAAAATGA             GTTTGGTGTT                                                                        -    721 TGGGAAATTT TTCTGCCTAA CAATGCAGAT GGTACATCAC CTATTCCTCA             TGGATCTCGT                                                                        -    781 GTAAAGGTGA GAATGGATAC TCCATCAGGG ATAAAGGATT CAATTCCAGC             CTGGATCAAG                                                                        -    841 TACTCAGTGC AGGCCCCAGG AGAAATACCA TATGATGGGA TTTATTATGA             TCCTCCTGAA                                                                        -    901 GAGGTAAAGT ATGTGTTCAG GCATGCGCAA CCTAAACGAC CAAAATCATT             GCGGATATAT                                                                        -    961 GAAACACATG TCGGAATGAG TAGCCCGGAA CCGAAGATAA ACACATATGT             AAACTTTAGG                                                                        -   1021 GATGAAGTCC TCCCAAGAAT AAAAAAACTT GGATACAATG CAGTGCAAAT             AATGGCAATC                                                                        -   1081 CAAGAGCACT CATATTATGG AAGCTTTGGA TACCATGTAA CTAATTTTTT             TGCGCCAAGT                                                                        -   1141 AGTCGTTTTG GTACCCCAGA AGATTTGAAG TCTTTGATTG ATAGAGCACA             TGAGCTTGGT                                                                        -   1201 TTGCTAGTTC TCATGGATGT GGTTCATAGT CATGCGTCAA GTAATACTCT             GGATGGGTTG                                                                        -   1261 AATGGTTTTG ATGGTACAGA TACACATTAC TTTCACAGTG GTCCACGTGG             CCATCACTGG                                                                        -   1321 ATGTGGGATT CTCGCCTATT TAACTATGGG AACTGGGAAG TTTTAAGATT             TCTTCTCTCC                                                                        -   1381 AATGCTAGAT GGTGGCTCGA GGAATATAAG TTTGATGGTT TCCGTTTTGA             TGGTGTGACC                                                                        -   1441 TCCATGATGT ACACTCACCA CGGATTACAA GTAACATTTA CGGGGAACTT             CAATGAGTAT                                                                        -   1501 TTTGGCTTTG CCACCGATGT AGATGCAGTG GTTTACTTGA TGCTGGTAAA             TGATCTAATT                                                                        -   1561 CATGGACTTT ATCCTGAGGC TGTAACCATT GGTGAAGATG TTAGTGGAAT             GCCTACATTT                                                                        -   1621 GCCCTTCCTG TTCACGATGG TGGGGTAGGT TTTGACTATC GGATGCATAT             GGCTGTGGCT                                                                        -   1681 GACAAATGGA TTGACCTTCT CAAGCAAAGT GATGAAACTT GGAAGATGGG             TGATATTGTG                                                                        -   1741 CACACACTGA CAAATAGGAG GTGGTTAGAG AAGTGTGTAA CTTATGCTGA             AAGTCATGAT                                                                        -   1801 CAAGCATTAG TCGGCGACAA GACTATTGCG TTTTGGTTGA TGGACAAGGA             TATGTATGAT                                                                        -   1861 TTCATGGCCC TCGATAGACC TTCAACTCCT ACCATTGATC GTGGGATAGC             ATTACATAAG                                                                        -   1921 ATGATTAGAC TTATCACAAT GGGTTTAGGA GGAGAGGGCT ATCTTAATTT             CATGGGAAAT                                                                        -   1981 GAGTTTGGAC ATCCTGAATG GATAGATTTT CCAAGAGGTC CGCAAAGACT             TCCAAGTGGT                                                                        -   2041 AAGTTTATTC CAGGGAATAA CAACAGTTAT GACAAATGTC GTCGAAGATT             TGACCTGGGT                                                                        -   2101 GATGCAGACT ATCTTAGGTA TCATGGTATG CAAGAGTTTG ATCAGGCAAT             GCAACATCTT                                                                        -   2161 GAGCAAAAAT ATGAATTCAT GACATCTGAT CACCAGTATA TTTCCCGGAA             ACATGAGGAG                                                                        -   2221 GATAAGGTGA TTGTGTTCGA AAAGGGAGAT TTGGTATTTG TGTTCAACTT             CCACTGCAAC                                                                        -   2281 AACAGCTATT TTGACTACCG TATTGGTTGT CGAAAGCCTG GGGTGTATAA             GGTGGTCTTG                                                                        -   2341 GACTCCGACG CTGGACTATT TGGTGGATTT AGCAGGATCC ATCACGCAGC             CGAGCACTTC                                                                        -   2401 ACCGCCGACT GTTCGCATGA TAATAGGCCA TATTCATTCT CGGTTTATAC             ACCAAGCAGA                                                                        -   2461 ACATGTGTCG TCTATGCTCC AGTGGAGTGA TAGCGGGGTA CTCGTTGCTG             CGCGGCATGT                                                                        -   2521 GTGGGGCTGT CGATGTGAGG AAAAACCTTC TTCCAAAACC GGCAGATGCA             TGCATGCATG                                                                        -   2581 CTACAATAAG GTTCTGATAC TTTAATCGAT GCTGGAAAGC CCATGCATCT             CGCTGCGTTG                                                                        -   2641 TCCTCTCTAT ATATATAAGA CCTTCAAGGT GTCAATTAAA CATAGAGTTT             TCGTTTTTCG                                                                        -   2701 CTTTCCTAAA AAAAAAAAAA AAAAA                                        __________________________________________________________________________

                                      TABLE 6                                      __________________________________________________________________________     mRNA Sequence and Deduced Amino Acid Sequence of the                             Maize Branching Enzyme I and the Transit Peptide                               [SEQ ID NO:16 and SEQ ID NO:17]                                              __________________________________________________________________________     LOCUS       MZEBEI       2763 bp ss-mRNA            PLN                          DEFINITION  Maize mRNA for branching enzyme-I (BE-I).                          ACCESSION   D11081                                                             KEYWORDS    branching enzyme-I.                                                SOURCE      Zea mays L. (inbred Oh43), cDNA to mRNA.                             ORGANISM  Zea mays                                                                       Eukaryota; Plantae; Embryobionta; Magnoliophyta; Liliopsida;                  Commelinidae; Liliopsida.                                          REFERENCE   1  (bases 1 to 2763)                                                 AUTHORS   Baba,T., Kimura,K., Mizuno,K., Etoh,H., Ishida,Y., Shida,O.       and                                                                                          Arai,Y.                                                              TITLE     Sequence conservation of the catalytic regions of Amylolytic                  enzymes in maize branching enzyme-I                                  JOURNAL   Biochem. Biophys. Res. Commun. 181, 87-94 (1991)                     STANDARD  full automatic                                                     COMMENT   Submitted (30-APR-1992) to DDBJ by: Tadashi Baba                                 Institute of Applied Biochemistry                                              University of Tsukuba                                                          Tsukuba, Ibaraki 305                                                           Japan                                                                          Phone:  0298-53-6632                                                           Fax:    0298-53-6632.                                                          NCBI gi: 217959                                                    FEATURES           Location/Qualifiers                                                source        1..2763                                                                        /organism="Zea mays"                                             CDS           <1..2470                                                                       /note="NCBI gi: 217960"                                                        /codon.sub.-- start=2                                                          /product="branching enzyme-I precursor"                    - /translation="LCLVSPSSSPTPLPPPRRSRSHADRAAPPGIAGGGNVRLSVLSV                   - QCKARRSGVRKVKSKFATAATVQEDKTMATAKGDVDHLPIYDLDPKLEIFKDHFRYRM                   - KRFLEQKGSIEENEGSLESFSKGYLKFGINTNEDGTVYREWAPAAQEAELIGDFNDWN                   - GANHKMEKDKFGVWSIKIDHVKGKPAIPHNSKVKFRFLHGGVWVDRIPALIRYATVDA                   - SKFGAPYDGVHWDPPASERYTFKHPRPSKPAAPRIYEAHVGMSGEKPAVSTYREFADN                   - VLPRIRANNYNTVQLMAVMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLVDKAHSLG                   - LRVLMDVVHSHASNNVTDGLNGYDVGQSTQESYFHAGDRGYHKLWDSRLFNYANWEVL                   - RFLLSNLRYWLDEFMFDGFRFDGVTSMLYHHHGINVGFTGNYQEYFSLDTAVDAVVYM                   - MLANHLMHKLLPEATVVAEDVSGMPVLCRPVDEGGVGFDYRLAMAIPDRWIDYLKNKD                   - DSEWSMGEIAHTLTNRRYTEKCIAYAESHDQSIVGDKTIAFLLMDKEMYTGMSDLQPA                   - SPTIDRGIALQKMIHFITMALGGDGYLNFMGNEFGHPEWIDFPREGNNWSYDKCRRQW                   - SLVDTDHLRYKYMNAFDQAMNALDERFSFLSSSKQIVSDMNDEEKVIVFERGDLVFVF                   - NFHPKKTYEGYKVGCDLPGKYRVALDSDALVFGGHGRVGHDVDHFTSPEGVPGVPETN                   - FNNRPNSFKVLSPPRTCVAYYRVDEAGAGRRLHAKAETGKTSPAESIDVKASRASSKE                   -                      DKEATAGGKKGWKFARQPSDQDTK"                               -      transit.sub.-- peptide 2..190                                               mat.sub.-- peptide     191..2467                                                               /EC.sub.-- number="2.4.1.18"                                                   /codon.sub.-- start=1                                                          /product="branching enzyme-I precursor"                        polyA.sub.-- signal    2734..2739                                         BASE COUNT      719 A     585 C    737 G    722 T                              ORIGIN                                                                              1 GCTGTGCCTC GTGTCGCCCT CTTCCTCGCC GACTCCGCTT CCGCCGCCGC GGCGCTCTCG        -     61 CTCGCATGCT GATCGGGCGG CACCGCCGGG GATCGCGGGT GGCGGCAATG              TGCGCCTGAG                                                                        -    121 TGTGTTGTCT GTCCAGTGCA AGGCTCGCCG GTCAGGGGTG CGGAAGGTCA             AGAGCAAATT                                                                        -    181 CGCCACTGCA GCTACTGTGC AAGAAGATAA AACTATGGCA ACTGCCAAAG             GCGATGTCGA                                                                        -    241 CCATCTCCCC ATATACGACC TGGACCCCAA GCTGGAGATA TTCAAGGACC             ATTTCAGGTA                                                                        -    301 CCGGATGAAA AGATTCCTAG AGCAGAAAGG ATCAATTGAA GAAAATGAGG             GAAGTCTTGA                                                                        -    361 ATCTTTTTCT AAAGGCTATT TGAAATTTGG GATTAATACA AATGAGGATG             GAACTGTATA                                                                        -    421 TCGTGAATGG GCACCTGCTG CGCAGGAGGC AGAGCTTATT GGTGACTTCA             ATGACTGGAA                                                                        -    481 TGGTGCAAAC CATAAGATGG AGAAGGATAA ATTTGGTGTT TGGTCGATCA             AAATTGACCA                                                                        -    541 TGTCAAAGGG AAACCTGCCA TCCCTCACAA TTCCAAGGTT AAATTTCGCT             TTCTACATGG                                                                        -    601 TGGAGTATGG GTTGATCGTA TTCCAGCATT GATTCGTTAT GCGACTGTTG             ATGCCTCTAA                                                                        -    661 ATTTGGAGCT CCCTATGATG GTGTTCATTG GGATCCTCCT GCTTCTGAAA             GGTACACATT                                                                        -    721 TAAGCATCCT CGGCCTTCAA AGCCTGCTGC TCCACGTATC TATGAAGCCC             ATGTAGGTAT                                                                        -    781 GAGTGGTGAA AAGCCAGCAG TAAGCACATA TAGGGAATTT GCAGACAATG             TGTTGCCACG                                                                        -    841 CATACGAGCA AATAACTACA ACACAGTTCA GTTGATGGCA GTTATGGAGC             ATTCGTACTA                                                                        -    901 TGCTTCTTTC GGGTACCATG TGACAAATTT CTTTGCGGTT AGCAGCAGAT             CAGGCACACC                                                                        -     961 AGAGGACCTC AAATATCTTG TTGATAAGGC ACACAGTTTG GGTTTGCGAG            TTCTGATGGA                                                                        -   1021 TGTTGTCCAT AGCCATGCAA GTAATAATGT CACAGATGGT TTAAATGGCT             ATGATGTTGG                                                                        -   1081 ACAAAGCACC CAAGAGTCCT ATTTTCATGC GGGAGATAGA GGTTATCATA             AACTTTGGGA                                                                        -   1141 TAGTCGGCTG TTCAACTATG CTAACTGGGA GGTATTAAGG TTTCTTCTTT             CTAACCTGAG                                                                        -   1201 ATATTGGTTG GATGAATTCA TGTTTGATGG CTTCCGATTT GATGGAGTTA             CATCAATGCT                                                                        -   1261 GTATCATCAC CATGGTATCA ATGTGGGGTT TACTGGAAAC TACCAGGAAT             ATTTCAGTTT                                                                        -   1321 GGACACAGCT GTGGATGCAG TTGTTTACAT GATGCTTGCA AACCATTTAA             TGCACAAACT                                                                        -   1381 CTTGCCAGAA GCAACTGTTG TTGCTGAAGA TGTTTCAGGC ATGCCGGTCC             TTTGCCGGCC                                                                        -   1441 AGTTGATGAA GGTGGGGTTG GGTTTGACTA TCGCCTGGCA ATGGCTATCC             CTGATAGATG                                                                        -   1501 GATTGACTAC CTGAAGAATA AAGATGACTC TGAGTGGTCG ATGGGTGAAA             TAGCGCATAC                                                                        -   1561 TTTGACTAAC AGGAGATATA CTGAAAAATG CATCGCATAT GCTGAGAGCC             ATGATCAGTC                                                                        -   1621 TATTGTTGGC GACAAAACTA TTGCATTTCT CCTGATGGAC AAGGAAATGT             ACACTGGCAT                                                                        -   1681 GTCAGACTTG CAGCCTGCTT CACCTACAAT TGATCGAGGG ATTGCACTCC             AAAAGATGAT                                                                        -   1741 TCACTTCATC ACAATGGCCC TTGGAGGTGA TGGCTACTTG AATTTTATGG             GAAATGAGTT                                                                        -   1801 TGGTCACCCA GAATGGATTG ACTTTCCAAG AGAAGGGAAC AACTGGAGCT             ATGATAAATG                                                                        -   1861 CAGACGACAG TGGAGCCTTG TGGACACTGA TCACTTGCGG TACAAGTACA             TGAATGCGTT                                                                        -   1921 TGACCAAGCG ATGAATGCGC TCGATGAGAG ATTTTCCTTC CTTTCGTCGT             CAAAGCAGAT                                                                        -   1981 CGTCAGCGAC ATGAACGATG AGGAAAAGGT TATTGTCTTT GAACGTGGAG             ATTTAGTTTT                                                                        -   2041 TGTTTTCAAT TTCCATCCCA AGAAAACTTA CGAGGGCTAC AAAGTGGGAT             GCGATTTGCC                                                                        -   2101 TGGGAAATAC AGAGTAGCCC TGGACTCTGA TGCTCTGGTC TTCGGTGGAC             ATGGAAGAGT                                                                        -   2161 TGGCCACGAC GTGGATCACT TCACGTCGCC TGAAGGGGTG CCAGGGGTGC             CCGAAACGAA                                                                        -   2221 CTTCAACAAC CGGCCGAACT CGTTCAAAGT CCTTTCTCCG CCCCGCACCT             GTGTGGCTTA                                                                        -   2281 TTACCGTGTA GACGAAGCAG GGGCTGGACG ACGTCTTCAC GCGAAAGCAG             AGACAGGAAA                                                                        -   2341 GACGTCTCCA GCAGAGAGCA TCGACGTCAA AGCTTCCAGA GCTAGTAGCA             AAGAAGACAA                                                                        -   2401 GGAGGCAACG GCTGGTGGCA AGAAGGGATG GAAGTTTGCG CGGCAGCCAT             CCGATCAAGA                                                                        -   2461 TACCAAATGA AGCCACGAGT CCTTGGTGAG GACTGGACTG GCTGCCGGCG             CCCTGTTAGT                                                                        -   2521 AGTCCTGCTC TACTGGACTA GCCGCCGCTG GCGCCCTTGG AACGGTCCTT             TCCTGTAGCT                                                                        -   2581 TGCAGGCGAC TGGTGTCTCA TCACCGAGCA GGCAGGCACT GCTTGTATAG             CTTTTCTAGA                                                                        -   2641 ATAATAATCA GGGATGGATG GATGGTGTGT ATTGGCTATC TGGCTAGACG             TGCATGTGCC                                                                        -   2701 CAGTTTGTAT GTACAGGAGC AGTTCCCGTC CAGAATAAAA AAAAACTTGT             TGGGGGGTTT                                                                        -   2761 TTC                                                                __________________________________________________________________________

                                      TABLE 7                                      __________________________________________________________________________     Coding Sequence and Deduced Amino Acid Sequence for                              Transit Peptide Region of the                                                  Soluble Starch Synthase I Maize Gene (153 bp)                                  [SEQ ID NO:18 and SEQ ID NO:19]                                                   FILE NAME   : MSS1TRPT.DNA   SEQUENCE : NORMAL   153 BP                      -      CODON TABLE : UNIV.TCN                                                  -      SEQUENCE    REGION :     1 -     153                                    -      TRANSLATION REGION :     1 -     153                                    -  *** DNA TRANSLATION ***                                                     -   1  ATG GCG ACG CCC TCG GCC GTG GGC GCC GCG TGC CTC CTC CTC GCG CGG         48                                                                             1   M   A   T   P   S   A   V   G   A   A   C   L   L   L   A   R           16                                                                                -  49  GCC GCC TGG CCG GCC GCC GTC GGC GAC CGG GCG CGC CCG CGG AGG CTC         96                                                                            17   A   A   W   P   A   A   V   G   D   R   A   R   P   R   R   L           32                                                                                -  97  CAG CGC GTG CTG CGC CGC CGG TGC GTC GCG GAG CTG AGC AGG GAG GGG        144                                                                            33   Q   R   V   L   R   R   R   C   V   A   E   L   S   R   E   G           48                                                                                - 145  CCC CAT ATG                                                           153                                                                             49   P   H   M                                                               __________________________________________________________________________     51                                                                        

GFP constructs

1. GFP only in pET-21a:

pEXS115 is digested with Nde I and Xho I and the 740 bp fragment containing the SGFP coding sequence is subdloned into the Nde I and Xho I sites of pET-21a (Novagen 601 Science Dr. Madison Wis.). (See PIG. 2b GFP-21a map.)

2. GFP subcloned in-frame at the 5' end of full-length mature WX:

The 740 bp Nde I fragment containing SGFP from pEXS114 is subcloned into the Nde I site of pEXSWX. (See FIG. 3a GFP-FLWX map.)

3. GFP subcloned in-frame at the 5' end of N-terminally truncated WX:

WX truncated by 700 bp at N-terminus.

The 1 kb BamHI fragment encoding the C-terminus of WX from pEXSWX is subcloned into the Bgl II site of pEXS115. Then the entire SGFP-truncated WX fragment is subcloned into pET21-L as a Nde 1-HindIII fragment. (See FIG. 3b GFP-BamHIWX map.)

4. GFP subcloned in-frame at the 5' end of truncated WX: WX truncated by 100 bp at N-terminus.

The 740 bp Nde I-Nco I fragment containing SGFP from pEXS115 is subcloned into pEXSWX at the Nde I and Nco I sites. (See FIG. 4 GFP-NcoWX map.)

Example Three

Plasmid Transformation into Bacteria:

Escherichia coli competent cell preparation:

1. Inoculate 2.5 ml LB media with a single colony of desired E. coli strain selected strain was XLIBLUE DL2IDE3 from (Stratagene); included appropriate antibiotics. Grow at 37° C., 250 rpm overnight.

2. Inoculate 100 ml of LB media with a 1:50 dilution of the overnight culture, including appropriate antibiotics. Grow at 37° C., 250 rpm until OD₆₀₀ =0.3-0.5.

3. Transfer culture to sterile centrifuge bottle and chill on ice for 15 minutes.

4. Centrifuge 5 minutes at 3,000× g (4° C.).

5. Resuspend pellet in 8 ml ice-cold Transformation buffer. Incubate on ice for 15 minutes.

6. Centrifuge 5 minutes at 3,000× g (4° C.).

7. Resuspend pellet in 8 ml ice-cold Transformation buffer 2. Aliquot, flash-freeze in liquid nitrogen, and stored at -70° C.

    ______________________________________                                         Transformation Buffer 1                                                                              Transformation Buffer 2                                  ______________________________________                                         RbCl    1.2      g        MOPS (10 mM)                                                                             0.209  g                                     MnCl.sub.2 4H.sub.2 O 0.99 g RbCl 0.12 g                                       K-Acetate 0.294 g CaCl.sub.2 2H.sub.2 O 1.1 g                                  CaCl.sub.2 2H.sub.2 O 0.15 g Glycerol 15 g                                     Glycerol 15 g dH.sub.2 O 100 ml                                              dH.sub.2 O                                                                             100      ml       pH to 6.8 with NaOH                                  pH to 5.8 with 0.2 M acetic acid                                                                 Filter sterilize                                               Filter sterilize                                                             ______________________________________                                    

Escherichia coli transformation by rubidium chloride heat shock method: Hanahan, D. (1985) in DNA cloning: a practical approach (Glover, D. M. ed.), pp. 109-135, IRL Press.

1. Incubate 1-5 μl of DNA on ice with 150 μl E. coli competent cells for 30 minutes.

2. Heat shock at 42° C. for 45 seconds.

3. Immediately place on ice for 2 minutes.

4. Add 600 μl LB media and incubate at 37° C. for 1 hour.

5. Plate on LB agar including the appropriate antibiotics.

This plasmid will express the hybrid polypeptide containing the green fluorescent protein within the bacteria.

Example Four

Expression of Construct in E. coli:

1. Inoculate 3 ml LB with E. coli containing plasmid of interest. Include appropriate antibiotics. 37° C., 250 rpm, overnight.

2. Inoculate 100 ml LB with 2 ml of overnight culture. Include appropriate antibiotics. Grow at 37° C., 250 rpm.

3. At OD₆₀₀ about 0.4-0.5, place at room temperature, 200 rpm.

4. At OD₆₀₀ about 0.6-0.8, induce with 100 μl 1 M 1PTG. Final 1PTG concentration is 1 mM.

5. Grow at room temperature, 200 rpm, 4-5 hours.

6. Collect cells by centrifugation.

7. Flash freeze in liquid nitrogen and store at -70° C. until use.

Cells can be resuspended in dH₂ O and viewed under UV light (γ_(max) =395 nm) for intrinsic fluorescence. Alternatively, the cells can be sonicated and an aliquot of the cell extract can be separated by SDS-PAGE and viewed under UV light to detect GFP fluorescence. When the protein employed is a green fluorescent protein, the presence of the protein in the lysed material can be evaluated under UV at 395 nm in a light box and the signature green glow car be identified.

Example Five

Plasmid Extraction from Bacteria

The following is one of many common alkaline lysis plasmid purification protocols useful in practicing this invention.

1. Inoculate 100-200 ml LB media with a single colony of E. coli transformed with the one of the plasmids described above. Include appropriate antibiotics. Grow at 37° C., 250 rpm overnight.

2. Centrifuge 10 minutes at 5,000× g (4° C.).

3. Resuspend cells in 10 ml water, transfer to a 15 ml centrifuge tube, and repeat centrifugation.

4. Resuspend pellet in 5 ml 0.1 M NaOH, 0.5% SDS. Incubate on ice for 10 minutes.

5. Add 2.5 ml of 3 M sodium acetate (pH 5.2), invert gently, and incubate 10 minutes on ice.

6. Centrifuge 5 minutes at 15,000-20,000× g (4° C.).

7. Extract supernatant with an equal volume of phenol: chloroform: isoamyl alcohol (25:24:1).

8. Centrifuge 10 minutes at 6,000-10,000× g (4° C.).

9. Transfer aqueous, phase to clean tube and precipitate with 1 volume of isopropanol.

10. Centrifuge 15 minutes at 12,000× g (4° C.).

11. Dissolve pellet in 0.5 ml TE, add 20 μl of 10 mg/ml Rnase, and incubate 1 hour at 37° C.

12. Extract twice with phenol:chloroform:isoamyl alcohol (25:24:1).

13. Extract once with chloroform.

14. Precipitate aqueous phase with 1 volume of isopropanol and 0.1 volume of 3 M sodium acetate.

15. Wash pellet once with 70% ethanol.

16. Dry pellet in SpeedVac and resuspend pellet in TE.

This plasmid can then be inserted into other hosts.

                                      TABLE 8                                      __________________________________________________________________________     DNA Sequence and Deduced Amino Acid Sequence of                                  Starch Synthase Coding Region from pEXS52 [SEQ ID NO:20; SEQ ID              __________________________________________________________________________     NO:21]                                                                               FILE  NAME   : MSS1DELN.DNA   SEQUENCE : NORMAL   1626 BP                   -       CODON TABLE : UNIV.TCN                                                 -       SEQUENCE    REGION :      1 -   1626                                   -       TRANSLATION REGION :      1 -   1626                                   -  -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:20:                              - TGC GTC GCG GAG CTG AGC AGG GAG GAC CTC GGT CTC GAA CCT GAA GGG                                                       48                                   Cys Val Ala Glu Leu Ser Arg Glu Asp Leu Gly Leu Glu Pro Glu Gly                                                                   55                                                               60                  65                     - ATT GCT GAA GGT TCC ATC GAT AAC ACA GTA GTT GTG GCA AGT GAG CAA                                                       96                                   Ile Ala Glu Gly Ser Ile Asp Asn Thr Val Val Val Ala Ser Glu Gln                                                               70                  75                                                              80                          - GAT TCT GAG ATT GTG GTT GGA AAG GAG CAA GCT CGA GCT AAA GTA ACA                                                      144                                   Asp Ser Glu Ile Val Val Gly Lys Glu Gln Ala Arg Ala Lys Val Thr                                                           85                  90                                                              95                              - CAA AGC ATT GTC TTT GTA ACC GGC GAA GCT TCT CCT TAT GCA AAG TCT                                                      192                                   Gln Ser Ile Val Phe Val Thr Gly Glu Ala Ser Pro Tyr Ala Lys Ser                                                      100                 105                                                             110                 115                                                    - GGG GGT CTA GGA GAT GTT TGT                                                GGT TCA TTG CCA GTT GCT CTT GCT                                                GCT          240                          Gly Gly Leu Gly Asp Val Cys Gly Ser Leu Pro Val Ala Leu Ala Ala                                                                      120                                                             125                 130                  - CGT GGT CAC CGT GTG ATG GTT GTA ATG CCC AGA TAT TTA AAT GGT ACC                                                      288                                   Arg Gly His Arg Val Met Val Val Met Pro Arg Tyr Leu Asn Gly Thr                                                                  135                                                               140                 145                    - TCC GAT AAG AAT TAT GCA AAT GCA TTT TAC ACA GAA AAA CAC ATT CGG                                                      336                                   Ser Asp Lys Asn Tyr Ala Asn Ala Phe Tyr Thr Glu Lys His Ile Arg                                                              150                 155                                                             160                          - ATT CCA TGC TTT GGC GGT GAA CAT GAA GTT ACC TTC TTC CAT GAG TAT                                                      384                                   Ile Pro Cys Phe Gly Gly Glu His Glu Val Thr Phe Phe His Glu Tyr                                                          165                 170                                                             175                              - AGA GAT TCA GTT GAC TGG GTG TTT GTT GAT CAT CCC TCA TAT CAC AGA                                                   432                                      Arg Asp Ser Val Asp Trp Val Phe Val Asp His Pro Ser Tyr His Arg                                                      180                 185                                                             190                 195                                                    - CCT GGA AAT TTA TAT GGA GAT                                                AAG TTT GGT GCT TTT GGT GAT AAT                                                CAG       480                             Pro Gly Asn Leu Tyr Gly Asp Lys Phe Gly Ala Phe Gly Asp Asn Gln                                                                      200                                                             205                 210                  - TTC AGA TAC ACA CTC CTT TGC TAT GCT GCA TGT GAG GCT CCT TTG ATC                                                   528                                      Phe Arg Tyr Thr Leu Leu Cys Tyr Ala Ala Cys Glu Ala Pro Leu Ile                                                                  215                                                               220                 225                    - CTT GAA TTG GGA GGA TAT ATT TAT GGA CAG AAT TGC ATG TTT GTT GTC                                                   576                                      Leu Glu Leu Gly Gly Tyr Ile Tyr Gly Gln Asn Cys Met Phe Val Val                                                              230                 235                                                             240                          - AAT GAT TGG CAT GCC AGT CTA GTG CCA GTC CTT CTT GCT GCA AAA TAT                                                   624                                      Asn Asp Trp His Ala Ser Leu Val Pro Val Leu Leu Ala Ala Lys Tyr                                                          245                 250                                                             255                              - AGA CCA TAT GGT GTT TAT AAA GAC TCC CGC AGC ATT CTT GTA ATA CAT                                                   672                                      Arg Pro Tyr Gly Val Tyr Lys Asp Ser Arg Ser Ile Leu Val Ile His                                                      260                 265                                                             270                 275                                                    - AAT TTA GCA CAT CAG GGT GTA                                                GAG CCT GCA AGC ACA TAT CCT GAC                                                CTT       720                             Asn Leu Ala His Gln Gly Val Glu Pro Ala Ser Thr Tyr Pro Asp Leu                                                                      280                                                             285                 290                  - GGG TTG CCA CCT GAA TGG TAT GGA GCT CTG GAG TGG GTA TTC CCT GAA                                                   768                                      Gly Leu Pro Pro Glu Trp Tyr Gly Ala Leu Glu Trp Val Phe Pro Glu                                                                  295                                                               300                 305                    - TGG GCG AGG AGG CAT GCC CTT GAC AAG GGT GAG GCA GTT AAT TTT TTG                                                   816                                      Trp Ala Arg Arg His Ala Leu Asp Lys Gly Glu Ala Val Asn Phe Leu                                                              310                 315                                                             320                          - AAA GGT GCA GTT GTG ACA GCA GAT CGA ATC GTG ACT GTC AGT AAG GGT                                                   864                                      Lys Gly Ala Val Val Thr Ala Asp Arg Ile Val Thr Val Ser Lys Gly                                                          325                 330                                                             335                              - TAT TCG TGG GAG GTC ACA ACT GCT GAA GGT GGA CAG GGC CTC AAT GAG                                                   912                                      Tyr Ser Trp Glu Val Thr Thr Ala Glu Gly Gly Gln Gly Leu Asn Glu                                                      340                 345                                                             350                 355                                                    - CTC TTA AGC TCC AGA AAG AGT                                                GTA TTA AAC GGA ATT GTA AAT GGA                                                ATT       960                             Leu Leu Ser Ser Arg Lys Ser Val Leu Asn Gly Ile Val Asn Gly Ile                                                                      360                                                             365                 370                  - GAC ATT AAT GAT TGG AAC CCT GCC ACA GAC AAA TGT ATC CCC TGT CAT                                                  1008                                      Asp Ile Asn Asp Trp Asn Pro Ala Thr Asp Lys Cys Ile Pro Cys His                                                                  375                                                               380                 385                    - TAT TCT GTT GAT GAC CTC TCT GGA AAG GCC AAA TGT AAA GGT GCA TTG                                                  1056                                      Tyr Ser Val Asp Asp Leu Ser Gly Lys Ala Lys Cys Lys Gly Ala Leu                                                              390                 395                                                             400                          - CAG AAG GAG CTG GGT TTA CCT ATA AGG CCT GAT GTT CCT CTG ATT GGC                                                     1104                                   Gln Lys Glu Leu Gly Leu Pro Ile Arg Pro Asp Val Pro Leu Ile Gly                                                          405                 410                                                             415                              - TTT ATT GGA AGG TTG GAT TAT CAG AAA GGC ATT GAT CTC ATT CAA CTT                                                     1152                                   Phe Ile Gly Arg Leu Asp Tyr Gln Lys Gly Ile Asp Leu Ile Gln Leu                                                      420                 425                                                             430                 435                                                    - ATC ATA CCA GAT CTC ATG CGG                                                GAA GAT GTT CAA TTT GTC ATG CTT                                                GGA         1200                          Ile Ile Pro Asp Leu Met Arg Glu Asp Val Gln Phe Val Met Leu Gly                                                                      440                                                             445                 450                  - TCT GGT GAC CCA GAG CTT GAA GAT TGG ATG AGA TCT ACA GAG TCG ATC                                                     1248                                   Ser Gly Asp Pro Glu Leu Glu Asp Trp Met Arg Ser Thr Glu Ser Ile                                                                  455                                                               460                 465                    - TTC AAG GAT AAA TTT CGT GGA TGG GTT GGA TTT AGT GTT CCA GTT TCC                                                     1296                                   Phe Lys Asp Lys Phe Arg Gly Trp Val Gly Phe Ser Val Pro Val Ser                                                              470                 475                                                             480                          - CAC CGA ATA ACT GCC GGC TGC GAT ATA TTG TTA ATG CCA TCC AGA TTC                                                     1344                                   His Arg Ile Thr Ala Gly Cys Asp Ile Leu Leu Met Pro Ser Arg Phe                                                          485                 490                                                             495                              - GAA CCT TGT GGT CTC AAT CAG CTA TAT GCT ATG CAG TAT GGC ACA GTT                                                     1392                                   Glu Pro Cys Gly Leu Asn Gln Leu Tyr Ala Met Gln Tyr Gly Thr Val                                                      500                 505                                                             510                 515                                                    - CCT GTT GTC CAT GCA ACT GGG                                                GGC CTT AGA GAT ACC GTG GAG AAC                                                TTC         1440                          Pro Val Val His Ala Thr Gly Gly Leu Arg Asp Thr Val Glu Asn Phe                                                                      520                                                             525                 530                  - AAC CCT TTC GGT GAG AAT GGA GAG CAG GGT ACA GGG TGG GCA TTC GCA                                                     1488                                   Asn Pro Phe Gly Glu Asn Gly Glu Gln Gly Thr Gly Trp Ala Phe Ala                                                                  535                                                               540                 545                    - CCC CTA ACC ACA GAA AAC ATG TTT GTG GAC ATT GCG AAC TGC AAT ATC                                                     1536                                   Pro Leu Thr Thr Glu Asn Met Phe Val Asp Ile Ala Asn Cys Asn Ile                                                              550                 555                                                             560                          - TAC ATA CAG GGA ACA CAA GTC CTC CTG GGA AGG GCT AAT GAA GCG AGG                                                     1584                                   Tyr Ile Gln Gly Thr Gln Val Leu Leu Gly Arg Ala Asn Glu Ala Arg                                                          565                 570                                                             575                              - CAT GTC AAA AGA CTT CAC GTG GGA CCA TGC CGC TGA                                                                     1620                                   His Val Lys Arg Leu His Val Gly Pro Cys Arg  *                                 580                 585                 590                                     -  - (2) INFORMATION FOR SEQ ID NO:21:                                         -         (i) SEQUENCE CHARACTERISTICS:                                                      (A) LENGTH: 540 amino acids                                                    (B) TYPE: amino acid                                                           (D) TOPOLOGY: linear                                             -        (ii) MOLECULE TYPE: protein                                           -        (xi) SEQUENCE DESCRIPTION: SEQ ID NO:21:                              - Cys Val Ala Glu Leu Ser Arg Glu Asp Leu Gly Leu Glu Pro Glu Gly                                                     1               5                                                              10                  15                  - Ile Ala Glu Gly Ser Ile Asp Asn Thr Val Val Val Ala Ser Glu Gln                                                                20                                                               25                  30                     - Asp Ser Glu Ile Val Val Gly Lys Glu Gln Ala Arg Ala Lys Val Thr                                                            35                  40                                                              45                          - Gln Ser Ile Val Phe Val Thr Gly Glu Ala Ser Pro Tyr Ala Lys Ser                                                        50                  55                                                              60                              - Gly Gly Leu Gly Asp Val Cys Gly Ser Leu Pro Val Ala Leu Ala Ala                                                    65                  70                                                              75                  80                                                    - Arg Gly His Arg Val Met Val                                                Val Met Pro Arg Tyr Leu Asn Gly                                                Thr                                                        85                  90                  95                     - Ser Asp Lys Asn Tyr Ala Asn Ala Phe Tyr Thr Glu Lys His Ile Arg                                                               100                                                               105                 110                    - Ile Pro Cys Phe Gly Gly Glu His Glu Val Thr Phe Phe His Glu Tyr                                                           115                 120                                                             125                          - Arg Asp Ser Val Asp Trp Val Phe Val Asp His Pro Ser Tyr His Arg                                                       130                 135                                                             140                              - Pro Gly Asn Leu Tyr Gly Asp Lys Phe Gly Ala Phe Gly Asp Asn Gln                                                   145                 150                                                             155                 160                                                    - Phe Arg Tyr Thr Leu Leu Cys                                                Tyr Ala Ala Cys Glu Ala Pro Leu                                                Ile                                                       165                 170                 175                     - Leu Glu Leu Gly Gly Tyr Ile Tyr Gly Gln Asn Cys Met Phe Val Val                                                               180                                                               185                 190                    - Asn Asp Trp His Ala Ser Leu Val Pro Val Leu Leu Ala Ala Lys Tyr                                                           195                 200                                                             205                          - Arg Pro Tyr Gly Val Tyr Lys Asp Ser Arg Ser Ile Leu Val Ile His                                                       210                 215                                                             220                              - Asn Leu Ala His Gln Gly Val Glu Pro Ala Ser Thr Tyr Pro Asp Leu                                                   225                 230                                                             235                 240                                                    - Gly Leu Pro Pro Glu Trp Tyr                                                Gly Ala Leu Glu Trp Val Phe Pro                                                Glu                                                       245                 250                 255                     - Trp Ala Arg Arg His Ala Leu Asp Lys Gly Glu Ala Val Asn Phe Leu                                                               260                                                               265                 270                    - Lys Gly Ala Val Val Thr Ala Asp Arg Ile Val Thr Val Ser Lys Gly                                                           275                 280                                                             285                          - Tyr Ser Trp Glu Val Thr Thr Ala Glu Gly Gly Gln Gly Leu Asn Glu                                                       290                 295                                                             300                              - Leu Leu Ser Ser Arg Lys Ser Val Leu Asn Gly Ile Val Asn Gly Ile                                                   305                 310                                                             315                 320                                                    - Asp Ile Asn Asp Trp Asn Pro                                                Ala Thr Asp Lys Cys Ile Pro Cys                                                His                                                       325                 330                 335                     - Tyr Ser Val Asp Asp Leu Ser Gly Lys Ala Lys Cys Lys Gly Ala Leu                                                               340                                                               345                 350                    - Gln Lys Glu Leu Gly Leu Pro Ile Arg Pro Asp Val Pro Leu Ile Gly                                                           355                 360                                                             365                          - Phe Ile Gly Arg Leu Asp Tyr Gln Lys Gly Ile Asp Leu Ile Gln Leu                                                       370                 375                                                             380                              - Ile Ile Pro Asp Leu Met Arg Glu Asp Val Gln Phe Val Met Leu Gly                                                   385                 390                                                             395                 400                                                    - Ser Gly Asp Pro Glu Leu Glu                                                Asp Trp Met Arg Ser Thr Glu Ser                                                Ile                                                       405                 410                 415                     - Phe Lys Asp Lys Phe Arg Gly Trp Val Gly Phe Ser Val Pro Val Ser                                                               420                                                               425                 430                    - His Arg Ile Thr Ala Gly Cys Asp Ile Leu Leu Met Pro Ser Arg Phe                                                           435                 440                                                             445                          - Glu Pro Cys Gly Leu Asn Gln Leu Tyr Ala Met Gln Tyr Gly Thr Val                                                       450                 455                                                             460                              - Pro Val Val His Ala Thr Gly Gly Leu Arg Asp Thr Val Glu Asn Phe                                                   465                 470                                                             475                 480                                                    - Asn Pro Phe Gly Glu Asn Gly                                                Glu Gln Gly Thr Gly Trp Ala Phe                                                Ala                                                       485                 490                 495                     - Pro Leu Thr Thr Glu Asn Met Phe Val Asp Ile Ala Asn Cys Asn Ile                                                               500                                                               505                 510                    - Tyr Ile Gln Gly Thr Gln Val Leu Leu Gly Arg Ala Asn Glu Ala Arg                                                           515                 520                                                             525                          - His Val Lys Arg Leu His Val Gly Pro Cys Arg  *                                  530                 535                 540                              __________________________________________________________________________

Example Six

This experiment employs a plasmid having a maize promoter, a maize transit peptide, a starch-encapsulating region from the starch synthase I gene, and a ligated gene fragment attached thereto. The plasmid shown in FIG. 6 contains the DNA sequence listed in Table 8.

Plasmid pEXS52 was constructed according to the following protocol:

Materials used to construct transgenic plasmids are as follows:

Plasmid pBluescript Sk-

Plasmid pMF6 (contain nos3' terminator)

Plasmid pHKH1 (contain maize adh 1 intron)

Plasmid MstsI(6-4) (contain maize stsI transit peptide, use as a template for PCT stsI transit peptide out)

Plasmid MstsIII in pBliuescript SK-

Primers EXS29 (GTGAGATCCATGGCGACGCCCTCGGCCGTGG) [SEQ ID NO:22]

EXS35 (CTGAYtTTCCATATGGGGCCCCTCCCTGCTCAGCTC) [SEQ ID NO:23] both used for P(CT stsI transit peptide

Primers EXS31 (CTCTGAGCTCAAGCTTGCTACTTTCTTTCCTTAATG) [SEQ ID NO:24]

EXS32 (GTCTCCGCGGTGGTGTCCTTGCTTCCTAG) [SEQ ID NO:25] both used for PCR maize 10 KD zein promoter (Journal: Gene 71:359-370 [1988]) Maize A632 genomic DNA (used as a template for PCR maize 10 KD zein promoter).

Step 1: Clone maize 10KD zein promoter in pBluescriptSK-(named as pEXS10 zp).

1. PCR 1.1 Kb maize 10 KD zein promoter

primers: EXS31, EXS32

template: maize A632 genomic DNA

2. Clone 1.1 Kb maize, 10 KD zein promoter PCR product into pBluescript SK-plasmid at SacI and SacII site (See FIG. 7).

Step 2: Delete BdeI site in pEXS10zp (named as pEXS10zp-NdeI).

NdeI is removed by fill in and blunt end ligation from maize 10 KD zein promoter in pBluescriptSK.

Step 3: Clone maize adh1 intron in pBluescriptSK- (named as pEXSadh1).

Maize adh1 intron is released from plasmid pHKH1 at XbaI and BamHI sites. Maize adh1 intron (XbaI/BamHI fragment) is cloned into pBluescriptSK- at XbaI and BamHI sites (see FIG. 7).

Step 4: Clone maize 10 KD zein promoter and maize adhl intron into pBluescriptSK-(named is pEXS10zp-adh 1).

Maize 10 KD zein promoter is released from plasmid pEXS 10zp-NdeI at SacI and SacII sites. Maize 10 KD zein promoter (SacI/SaclI fragment) is cloned into plasmid pEXSadh1 (contain maize adh1 intron) at SacI and SacII sites (see FIG. 7).

Step 5: Clone maize nos3' terminator into plasmid pEXSadh1 (named as pEXSadh1-nos3').

Maize nos3' terminator is released from plasmid pMF6 at EcoRI and HindIII sites. Maize nos3' terminator (EcoRI/HindIII fragment) is cloned into plasmid pEXSadh1 at EcoRI and HindIII (see FIG. 7).

Step 6: Clone maize nos3' terminator into plasmid pEXS10zp-adh1 (named as pEXS10zp-adh1-nos3').

Maize nos3' terminator is released from plasmid pEXSadh1-nos3' at EcoRI and ApaI sites. Maize nos3' terminator (EcoRI/ApaI fragment) is cloned into plasmid pEXS10zp-adh1 at EcoRI and ApaI sites (see FIG. 7).

Step 7: Clone maize STSI transit peptide into plasmid pEXS10zp-adh1-nos3' (named as pEXS33).

1. PCR 150 bp maize STSI transit peptide

primer: EXS29, EXS35

template: MSTSI(6-4) plasmid

2. Clone 150 bp maize STSI transit peptide PCR product into plasmid pEXS10zp-adh1-nos3' at EcoRI and BamHI sites (see FIG. 7).

Step 8: Site-directed mutagenesis on maize STSI transit peptide in pEXS33 (named as pEXS33(m)).

There is a mutation (stop codon) on maize STSI transit peptide in plasmid pEXS33. Site-directed mutagenesis is carried out to change stop codon to non-stop codon. New plasmid (containing maize 10 KD zein promoter, maize STSI transit peptide, maize adh1 intron, maize nos3' terminator) is named as pEXS33(m).

Step 9: NotI site in pEXS33(m) deleted (named as pEXS50).

NotI site is removed from pEXS33 by NotI fillin, blunt end ligation to form pEXS50 (see FIG. 8).

Step 10: Maize adh1 intron deleted in pEXS33(m) (named as pEXS60).

Maize adh1 intron is removed by NotI/BamHI digestion, filled in with Klenow fragment, blunt end ligation to form pEXS60 (see FIG. 9).

Step 11: Clone maize STSIII into pEXS50, pEXS60.

Maize STSIII is released from plasmid maize STSIII in pBluescript SK- at NdeI and EcoRI sites. Maize STSIII (NdeI-EcoRI fragment) is cloned into pEXS50, pEXS60 separately, named as pEXS51, pEXS61 (see FIGS. 8 and 9, respectively).

Step 12: Clone the gene in Table 8 into pEXS51 at NdeI/NotI site to form pEXS52. Other similar plasmids can be made by cloning other genes (STSI, II, WX, glgA, glgB, glgC, BEI, BEII, etc.) into pEXS51, pEXS61 at NdeI/NotI site.

Plasmid EXS52 was transformed into rice. The regenerated rice plants transformed with pEXS52 were marked and placed in a magenta box.

Two siblings of each line were chosen from the magenta box and transferred into 2.5 inch pots filled with soil mix (topsoil mixed with peat-vermiculite 50/50). The pots were placed in an aquarium (fish tank) with half an inch of water. The top was covered to maintain high humidity, (some holes were made to help heat escape). A thermometer monitored the temperature. The fish tank was placed under fluorescent lights. No fertilizer was used on the plants in the first week. Light period was 6 a.m.-8 p.m., minimum 14 hours light. Temperature was minimum 68° F. at night, 80°-90° F. during the day. A heating mat was used under the fish tank to help root growth when necessary. The plants stayed in the above condition for a week. (Note: the seedlings began to grow tall because of low light intensity.)

After the first week, the top of the aquarium was opened and rice transformants were transferred to growth chambers for three weeks with high humidity and high light intensity.

Alternatively, water mix in the greenhouse can be used to maintain high humidity. The plants grew for three weeks. Then the plants were transferred to 6-inch pots (minimum 5-inch pots) with soil mix (topsoil and peat-Vet, 50/50). The pots were in a tray filled with half an inch of water. 15-16-17 (N-K-P) was used to fertilize the plants (250 ppm) once a week or according to the plants' needs by their appearances. The plants remained in 14 hours light (minimum) 6 a.m.-8 p.m. high light intensity, temperature 85°-90°/70° F. day/night.

The plants formed rice grains and the rice grains were harvested. These harvested seeds can have the starch extracted and analyzed for the presence of the ligated amino acids C, V, A, E, L, S, R, E [SEQ ID NO:27] in the starch within the seed.

Example Seven

SER Vector for Plains

The plasmid shown in FIG. 6 is adapted for use in monocots, i.e., maize. Plasmid pEXS52 (FIG. 6) has a promoter, a transit peptide (from maize), and a ligated gene fragment (TGC GTC GCG GAG CTG AGC AGG GAG) [SEQ ID NO:26] which encodes the amino acid sequence C V A E L S R E [SEQ ID NO:27].

This gene fragment naturally occurs close to the N-terminal end of the maize soluble starch synthase (MSTSI) gene. As is shown in TABLE 8, at about amino acid 292 the SER from the starch synthase begins. This vector is preferably transformed into a maize host. The transit peptide is adapted for maize so this is the preferred host. Clearly the transit peptide and the promoter, if necessary, can be altered to be appropriate for the host plant desired. After transformation by "whiskers" technology (U.S. Pat. Nos. 5,302,523 and 5,464,765), the transformed host cells are regenerated by methods known in the art, the transformant is pollinated, and the resultant kernels can be collected and analyzed for the presence of the peptide in the starch and the starch granule.

The following preferred genes can be employed in maize to improve feeds: phytase gene, the somototrophin gene, the following chained amino acids: AUG AUG AUG AUG AUG AUG AUG AUG [SEQ ID NO:28]; and/or, AAG AAG AAG AAG AAG AAG AAG AAG AAG AAG AAG AAG {SEQ ID NO:29]; and/or AAA AAA AAA AAA AAA AAA [SEQ ID NO:30]; or a combination of the codons encoding the lysine amino acid in a chain or a combination of the codons encoding both lysine and the methionine codon or any combination of two or three of these amino acids. The length of the chains should not be unduly long but the length of the chain does not appear to be critical. Thus the amino acids will be encapsulated within the starch granule or bound within the starch formed in the starch-bearing portion of the plant host.

This plasmid may be transformed into other cereals such as rice, wheat, barley, oats, sorghum, or millet with little to no modification of the plasmid. The promoter may be the waxy gene promoter whose sequence has been published, or other zein promoters known to the art.

Additionally these plasmids, without undue experimentation, may be transformed into dicots such as potatoes, sweet potato, taro, yam, lotus cassava, peanuts, peas, soybean, beans, or chickpeas. The promoter may be selected to target the starch-storage area of particular dicots or tubers, for example the patatin promoter may be used for potato tubers.

Various methods of transforming monocots and dicots are known in the industry and the method of transforming the genes is not critical to the present invention. The plasmid can be introduced into Agrobacterium tumefaciens by the freeze-thaw method of An et al. (1988) Binary Vectors, in Plant Molecular Biology Manual A3, S. B. Gelvin and R. A. Schilperoot, eds. (Dordrecht, The Netherlands: Kluwer Academic Publishers), pp. 1-19. Preparation of Agrobacterium inoculum carrying the construct and inoculation of plant material, regeneration of shoots, and rooting of shoots are described in Edwards et al., "Biochemical and molecular characterization of a navel starch synthase from potatoes," Plant J. 8, 283-294 (1995).

A number of encapsulating regions are present in a number of different genes. Although it is preferred that the protein be encapsulated within the starch granule (granule encapsulation), encapsulation within non-granule starch is also encompassed within the scope of the present invention in the term "encapsulation. " The following types of genes are useful for this purpose.

Use of Starch-Encapsulating Regions of Glycogen Synthase

E. coli glycogen synthase is not a large protein: the structural gene is 1431 base pairs in length, specifying a protein of 477 amino acids with an estimated molecular weight of 49,000. It is known that problems of codon usage can occur with bacterial genes inserted into plant genomes but this is generally not so great with E. coli genes as with those from other bacteria such as those from Bacillus. Glycogen synthase from E. coli has a codon usage profile much in common with maize genes but it is preferred to alter, by known procedures, the sequence at the translation start point to be more compatible with a plant consensus sequence:

glgA G A T A A T G C A G [SEQ ID NO:31]

cons A A C A A T G G C T [SEQ ID NO:32]

Use of Starch-Encapsulating Regions of Soluble Starch Synthase

cDNA clones of plant-soluble starch synthases are described in the background section above and can be used in the present invention. The genes for any such SSTS protein may be used in constructs according to this invention.

Use of Starch-Encapulating Regions of Branching Enzyme

cDNA clones of plant, bacterial and animal branching enzymes are described in the background section above can be used in the present invention. Branching enzyme [1,4Dglucan: 1,4Dglucan 6D(1,4Dglucano) transferase (E.C. 2.4.1.18)] converts amylose to amylopectin, (a segment of a 1,4Dglucan chain is transferred to a primary hydroxyl group in a similar glucan chain) sometimes called Q-enzyme.

The sequence of maize branching enzyme I was investigated by Baba et al. (1991) BBRC, 181:87-94. Starch branching enzyme II from maize endosperm was investigated by Fisher et al. (1993) Plant Physiol, 102:1045-1046. The BE gene construct may require the presence of an amyloplast transit peptide to ensure its correct localization in the amyloplast. The genes for any such branching enzyme of GBSTS protein may be used in constructs according to this invention.

Use of Starch-Binding Domains of Granule-Bound Starch Synthase

The use of cDNA clones of plant granule-bound starch synthases are described in Shure et al. (1983) Cell 35:225-233, and Visser et al. (1989) Plant Sci. 64(2):185-192. Visser et al. have also described the inhibition of the expression of the gene for granule-bound starch synthase in potato by antisense constructs (1991) Mol. Gen. Genetic 225(2):289-296; (1994) The Plant Cell 6:43-52.) Shimada et al. show antisense in rice (1993) Theor. Appl. Genet. 86:665-672. Van der Leij et al. show restoration of amylose synthesis in low-amylose potato following transformation with the wild-type waxy potato gene (1991) Theor. Appl. Genet. 82:289-295.

The amino acid sequences and nucleotide sequences of granule starch synthases from, for example, maize, rine, wheat, potato, cassava, peas or barley are well known. The genes for any such GBSTS protein may be used in constructs according to this invention.

Construction of Plant Transformation Vectors

Plant transformation vectors for use in the method of the invention may be constructed using standard techniques

Use of Transit Peptide Sequences

Some gene constructs require the presence of an amyloplast transit peptide to ensure correct localization in the amyloplast. It is believed that chloroplast transit peptides have similar sequences (Heijne et al. describe a database of chloroplast transit peptides in (1991) Plant Mol. Biol. Reporter, 9(2): 104-126). Other transit peptides useful in this invention are those of ADPG pyrophosphorylase (1991) Plant Mol. Biol. Reporter, 9:104-126), small subunit RUBISCO, aceiolactate synthase, glyceraldehyde3Pdehydrogenase and nitrite reductase.

The consensus sequence of the transit peptide of small subunit RUBISCO from many genotypes has the sequence:

MASSMLSSAAVATRTNPAQASM VAPFTGLKSAAFPVSRKQNLDI TSIASNGGRVQC [SEQ ID NO:33]

The corn small subunit RUBISCO has the sequence:

MAPTVMMASSATAVTRTNPAQAS AVAPFQGLKSTASLPVARRSSR SLGNVASNGGRIRC [SEQ ID NO:34]

The transit peptide of leaf glyceraldehyde3Pdehydrogenase from corn has the sequence:

MAQILAPSTQWQMRITKTSPCA TPITSKMWSSLVMKQTKKVAHS AKFRVMAVNSENGT [SEQ ID NO:35]

The transit peptide sequence of corn endosperm-bound starch synthase has the sequence:

MAALATSQLVATRAGHGVPDASTFRRGAAQGLRGARASAAADTLSMRTSARAAPRHQ QQARRGGRFPFPSLVVC [SEQ ID NO:36]

The transit peptide sequence of corn endosperm soluble starch synthase has the sequence:

MATPSAVGAACLLLARXAWPAAVGDRARPRRLQRVLRRR [SEQ ID NO:37]

Engineering New Amino Acids or Peptides into Starch-Encapsulating Proteins

The starch-binding proteins used in this invention may be modified by methods known to those skilled in the art to incorporate new amino acid combinations. For example, sequences of starch-binding proteins may be modified to express higher-than-normal levels of lysine, methionine or tryptophan. Such levels can be usefully elevated above natural levels and such proteins provide nutritional enhancement in crops such as cereals.

In addition to altering amino acid balance, it is possible to engineer the starch-binding proteins so that valuable peptides can be incorporated into the starch-binding protein. Attaching the payload polypeptide to the starch-binding protein at the N-terminal end of the protein provides a known means of adding peptide fragments and still maintaining starch-binding capacity. Further improvements can be made by incorporating specific protease cleavage sites into the site of attachment of the payload polypeptide to the starch-encapsulating region. It is well known to those skilled in the art that proteases have preferred specificities for different amino-acid linkages. Such specificities can be used to provide a vehicle for delivery of valuable peptides to different regions of the digestive tract of animals and man.

In yet another embodiment of this invention, the payload polypeptide can be released following purification and processing of the starch granules. Using amylolysis and/or gelatinization procedures it is known that the proteins bound to the starch granule can be released or become available for proteolysis. Thus recovery of commercial quantities of proteins and peptides from the starch granule matrix becomes possible.

In yet another embodiment of the invention it is possible to process the starch granules in a variety of different ways in order to provide a means of altering the digestibility of the starch. Using this methodology it is possible to change the bioavailablility of the proteins, peptides or amino acids entrapped within the starch granules.

Although the foregoing invention has been described in detail by way of illustration and example for purposes of clarity and understanding, it will be readily apparent to those of ordinary skill in the art in light of the teachings of this invention that certain changes and modifications may be made thereto without departing from the spirit or scope of the appended claims.

    __________________________________________________________________________     #             SEQUENCE LISTING                                                    - -  - - (1) GENERAL INFORMATION:                                              - -    (iii) NUMBER OF SEQUENCES: 37                                           - -  - - (2) INFORMATION FOR SEQ ID NO:1:                                      - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 31 base - #pairs                                                   (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                  - -     (ii) MOLECULE TYPE: other nucleic acid                                          (A) DESCRIPTION: /desc - #= "Oligonucleotide"                         - -    (iii) HYPOTHETICAL: NO                                                  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:1:                                - - GACTAGTCAT ATGGTGAGCA AGGGCGAGGA G        - #                  - #               31                                                                      - -  - - (2) INFORMATION FOR SEQ ID NO:2:                                      - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 36 base - #pairs                                                   (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                  - -     (ii) MOLECULE TYPE: other nucleic acid                                          (A) DESCRIPTION: /desc - #= "Oligonucleotide"                         - -    (iii) HYPOTHETICAL: NO                                                  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:2:                                - - CTAGATCTTC ATATGCTTGT ACAGCTCGTC CATGCC      - #                  -      #       36                                                                       - -  - - (2) INFORMATION FOR SEQ ID NO:3:                                      - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 39 base - #pairs                                                   (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                  - -     (ii) MOLECULE TYPE: other nucleic acid                                          (A) DESCRIPTION: /desc - #= "Oligonucleotide"                         - -    (iii) HYPOTHETICAL: NO                                                  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:3:                                - - CTAGATCTTG GCCATGGCCT TGTACAGCTC GTCCATGCC      - #                       - #    39                                                                       - -  - - (2) INFORMATION FOR SEQ ID NO:4:                                      - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 4800 base - #pairs                                                 (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: double                                                       (D) TOPOLOGY: Not Relev - #ant                                        - -     (ii) MOLECULE TYPE: DNA (genomic)                                      - -    (iii) HYPOTHETICAL: NO                                                  - -     (vi) ORIGINAL SOURCE:                                                           (A) ORGANISM: Zea mays                                                - -     (ix) FEATURE:                                                                   (A) NAME/KEY: CDS                                                              (B) LOCATION: join(1449..1 - #553, 1685..1765, 1860..1958,          2055                                                                                            ..2144, 2 - #226..2289, 2413..2513, 2651..2760, 2858                          ..3101, 3 - #212..3394, 3490..3681, 3793..3879, 3977                           ..4105, 4 - #227..4343)                                          - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:4:                                - - CAGCGACCTA TTACACAGCC CGCTCGGGCC CGCGACGTCG GGACACATCT TC -              #TTCCCCCT     60                                                                  - - TTTGGTGAAG CTCTGCTCGC AGCTGTCCGG CTCCTTGGAC GTTCGTGTGG CA -             #GATTCATC    120                                                                  - - TGTTGTCTCG TCTCCTGTGC TTCCTGGGTA GCTTGTGTAG TGGAGCTGAC AT -             #GGTCTGAG    180                                                                  - - CAGGCTTAAA ATTTGCTCGT AGACGAGGAG TACCAGCACA GCACGTTGCG GA -             #TTTCTCTG    240                                                                  - - CCTGTGAAGT GCAACGTCTA GGATTGTCAC ACGCCTTGGT CGCGTCGCGT CG -             #CGTCGCGT    300                                                                  - - CGATGCGGTG GTGAGCAGAG CAGCAACAGC TGGGCGGCCC AACGTTGGCT TC -             #CGTGTCTT    360                                                                  - - CGTCGTACGT ACGCGCGCGC CGGGGACACG CAGCAGAGAG CGGAGAGCGA GC -             #CGTGCACG    420                                                                  - - GGGAGGTGGT GTGGAAGTGG AGCCGCGCGC CCGGCCGCCC GCGCCCGGTG GG -             #CAACCCAA    480                                                                  - - AAGTACCCAC GACAAGCGAA GGCGCCAAAG CGATCCAAGC TCCGGAACGC AA -             #CAGCATGC    540                                                                  - - GTCGCGTCGG AGAGCCAGCC ACAAGCAGCC GAGAACCGAA CCGGTGGGCG AC -             #GCGTCATG    600                                                                  - - GGACGGACGC GGGCGACGCT TCCAAACGGG CCACGTACGC CGGCGTGTGC GT -             #GCGTGCAG    660                                                                  - - ACGACAAGCC AAGGCGAGGC AGCCCCCGAT CGGGAAAGCG TTTTGGGCGC GA -             #GCGCTGGC    720                                                                  - - GTGCGGGTCA GTCGCTGGTG CGCAGTGCCG GGGGGAACGG GTATCGTGGG GG -             #GCGCGGGC    780                                                                  - - GGAGGAGAGC GTGGCGAGGG CCGAGAGCAG CGCGCGGCCG GGTCACGCAA CG -             #CGCCCCAC    840                                                                  - - GTACTGCCCT CCCCCTCCGC GCGCGCTAGA AATACCGAGG CCTGGACCGG GG -             #GGGGGCCC    900                                                                  - - CGTCACATCC ATCCATCGAC CGATCGATCG CCACAGCCAA CACCACCCGC CG -             #AGGCGACG    960                                                                  - - CGACAGCCGC CAGGAGGAAG GAATAAACTC ACTGCCAGCC AGTGAAGGGG GA -             #GAAGTGTA   1020                                                                  - - CTGCTCCGTC GACCAGTGCG CGCACCGCCC GGCAGGGCTG CTCATCTCGT CG -             #ACGACCAG   1080                                                                  - - GTTCTGTTCC GTTCCGATCC GATCCGATCC TGTCCTTGAG TTTCGTCCAG AT -             #CCTGGCGC   1140                                                                  - - GTATCTGCGT GTTTGATGAT CCAGGTTCTT CGAACCTAAA TCTGTCCGTG CA -             #CACGTCTT   1200                                                                  - - TTCTCTCTCT CCTACGCAGT GGATTAATCG GCATGGCGGC TCTGGCCACG TC -             #GCAGCTCG   1260                                                                  - - TCGCAACGCG CGCCGGCCTG GGCGTCCCGG ACGCGTCCAC GTTCCGCCGC GG -             #CGCCGCGC   1320                                                                  - - AGGGCCTGAG GGGGGCCCGG GCGTCGGCGG CGGCGGACAC GCTCAGCATG CG -             #GACCAGCG   1380                                                                  - - CGCGCGCGGC GCCCAGGCAC CAGCAGCAGG CGCGCCGCGG GGGCAGGTTC CC -             #GTCGCTCG   1440                                                                  - - TCGTGTGC GCC AGC GCC GGC ATG AAC GTC GTC TTC - #GTC GGC GCC GAG         ATG    1490                                                                               Ala Ser Ala Gly Met A - #sn Val Val Phe Val Gly Ala Glu Met                     1       - #        5          - #        10                          - - GCG CCG TGG AGC AAG ACC GGC GGC CTC GGC GA - #C GTC CTC GGC GGC CTG          1538                                                                        Ala Pro Trp Ser Lys Thr Gly Gly Leu Gly As - #p Val Leu Gly Gly Leu             15                 - # 20                 - # 25                 - # 30        - - CCG CCG GCC ATG GCC GTAAGCGCGC GCACCGAGAC ATGCATCCG - #T TGGATCGCGT          1593                                                                        Pro Pro Ala Met Ala                                                                             35                                                             - - CTTCTTCGTG CTCTTGCCGC GTGCATGATG CATGTGTTTC CTCCTGGCTT GT -              #GTTCGTGT   1653                                                                  - - ATGTGACGTG TTTGTTCGGG CATGCATGCA G GCG AAC GGG CAC - #CGT GTC ATG           1705                                                                                          - #                 Ala - # Asn Gly His Arg Val Met                            - #                  - #                40                    - - GTC GTC TCT CCC CGC TAC GAC CAG TAC AAG GA - #C GCC TGG GAC ACC AGC          1753                                                                        Val Val Ser Pro Arg Tyr Asp Gln Tyr Lys As - #p Ala Trp Asp Thr Ser                     45         - #         50         - #         55                       - - GTC GTG TCC GAG GTACGGCCAC CGAGACCAGA TTCAGATCAC AG - #TCACACAC              1805                                                                        Val Val Ser Glu                                                                     60                                                                         - - ACCGTCATAT GAACCTTTCT CTGCTCTGAT GCCTGCAACT GCAAATGCAT GC - #AG ATC          1862                                                                                          - #                  - #                  - #      Ile        - - AAG ATG GGA GAC GGG TAC GAG ACG GTC AGG TT - #C TTC CAC TGC TAC AAG          1910                                                                        Lys Met Gly Asp Gly Tyr Glu Thr Val Arg Ph - #e Phe His Cys Tyr Lys                 65             - #     70             - #     75                           - - CGC GGA GTG GAC CGC GTG TTC GTT GAC CAC CC - #A CTG TTC CTG GAG AGG          1958                                                                        Arg Gly Val Asp Arg Val Phe Val Asp His Pr - #o Leu Phe Leu Glu Arg             80                 - # 85                 - # 90                 - # 95        - - GTGAGACGAG ATCTGATCAC TCGATACGCA ATTACCACCC CATTGTAAGC AG -              #TTACAGTG   2018                                                                  - - AGCTTTTTTT CCCCCCGGCC TGGTCGCTGG TTTCAG GTT TGG GGA - #AAG ACC GAG          2072                                                                                          - #                  - #    Val Trp Gly Lys Thr Glu                            - #                  - #                  - #  100            - - GAG AAG ATC TAC GGG CCT GTC GCT GGA ACG GA - #C TAC AGG GAC AAC CAG          2120                                                                        Glu Lys Ile Tyr Gly Pro Val Ala Gly Thr As - #p Tyr Arg Asp Asn Gln                        105      - #           110      - #           115                   - - CTG CGG TTC AGC CTG CTA TGC CAG GTCAGGATGG CT - #TGGTACTA CAACTTCATA         2174                                                                        Leu Arg Phe Ser Leu Leu Cys Gln                                                        120          - #       125                                              - - TCATCTGTAT GCAGCAGTAT ACACTGATGA GAAATGCATG CTGTTCTGCA G - #GCA GCA         2231                                                                                           - #                  - #                  - #   Ala         Ala                                                                               - - CTT GAA GCT CCA AGG ATC CTG AGC CTC AAC AA - #C AAC CCA TAC TTC         TCC     2279                                                                     Leu Glu Ala Pro Arg Ile Leu Ser Leu Asn As - #n Asn Pro Tyr Phe Ser                   130          - #       135          - #       140                       - - GGA CCA TAC G GTAAGAGTTG CAGTCTTCGT ATATATATCT GTTG - #AGCTCG                2329                                                                        Gly Pro Tyr                                                                        145                                                                         - - AGAATCTTCA CAGGAAGCGG CCCATCAGAC GGACTGTCAT TTTACACTGA CT -              #ACTGCTGC   2389                                                                  - - TGCTCTTCGT CCATCCATAC AAG  GG GAG GAC GTC GTG - #TTC GTC TGC AAC            2438                                                                                          - #        Gly Glu Asp Val Val Phe - #Val Cys Asn                              - #                  - #  150               - #  155          - - GAC TGG CAC ACC GGC CCT CTC TCG TGC TAC CT - #C AAG AGC AAC TAC CAG          2486                                                                        Asp Trp His Thr Gly Pro Leu Ser Cys Tyr Le - #u Lys Ser Asn Tyr Gln                            160  - #               165  - #               170               - - TCC CAC GGC ATC TAC AGG GAC GCA AAG GTTGCCTTC - #T CTGAACTGAA                2533                                                                        Ser His Gly Ile Tyr Arg Asp Ala Lys                                                        175      - #           180                                          - - CAACGCCGTT TTCGTTCTCC ATGCTCGTAT ATACCTCGTC TGGTAGTGGT GG -              #TGCTTCTC   2593                                                                  - - TGAGAAACTA ACTGAAACTG ACTGCATGTC TGTCTGACCA TCTTCACGTA CT - #ACCAG           2650                                                                        - - ACC GCT TTC TGC ATC CAC AAC ATC TCC TAC CA - #G GGC CGG TTC GCC TTC          2698                                                                        Thr Ala Phe Cys Ile His Asn Ile Ser Tyr Gl - #n Gly Arg Phe Ala Phe                            185  - #               190  - #               195               - - TCC GAC TAC CCG GAG CTG AAC CTC CCG GAG AG - #A TTC AAG TCG TCC TTC          2746                                                                        Ser Asp Tyr Pro Glu Leu Asn Leu Pro Glu Ar - #g Phe Lys Ser Ser Phe                        200      - #           205      - #           210                   - - GAT TTC ATC GAC GG  GTCTGTTTTC CTGCGTGCAT GTGAAC - #ATTC ATGAATGGTA          2800                                                                        Asp Phe Ile Asp Gly                                                                    215                                                                     - - ACCCACAACT GTTCGCGTCC TGCTGGTTCA TTATCTGACC TGATTGCATT AT - #TGCAG       C    2858                                                                         - - TAC GAG AAG CCC GTG GAA GGC CGG AAG ATC AA - #C TGG ATG AAG GCC         GGG     2906                                                                     Tyr Glu Lys Pro Val Glu Gly Arg Lys Ile As - #n Trp Met Lys Ala Gly                   220          - #       225          - #       230                       - - ATC CTC GAG GCC GAC AGG GTC CTC ACC GTC AG - #C CCC TAC TAC GCC GAG          2954                                                                        Ile Leu Glu Ala Asp Arg Val Leu Thr Val Se - #r Pro Tyr Tyr Ala Glu                235              - #   240              - #   245                           - - GAG CTC ATC TCC GGC ATC GCC AGG GGC TGC GA - #G CTC GAC AAC ATC ATG          3002                                                                        Glu Leu Ile Ser Gly Ile Ala Arg Gly Cys Gl - #u Leu Asp Asn Ile Met            250                 2 - #55                 2 - #60                 2 -       #65                                                                               - - CGC CTC ACC GGC ATC ACC GGC ATC GTC AAC GG - #C ATG GAC GTC AGC         GAG     3050                                                                     Arg Leu Thr Gly Ile Thr Gly Ile Val Asn Gl - #y Met Asp Val Ser Glu                           270  - #               275  - #               280               - - TGG GAC CCC AGC AGG GAC AAG TAC ATC GCC GT - #G AAG TAC GAC GTG TCG          3098                                                                        Trp Asp Pro Ser Arg Asp Lys Tyr Ile Ala Va - #l Lys Tyr Asp Val Ser                        285      - #           290      - #           295                   - - ACG GTGAGCTGGC TAGCTCTGAT TCTGCTGCCT GGTCCTCCTG CTCATCATG - #C               3151                                                                        Thr                                                                             - - TGGTTCGGTA CTGACGCGGC AAGTGTACGT ACGTGCGTGC GACGGTGGTG TC -              #CGGTTCAG   3211                                                                  - - GCC GTG GAG GCC AAG GCG CTG AAC AAG GAG GC - #G CTG CAG GCG GAG         GTC     3259                                                                     Ala Val Glu Ala Lys Ala Leu Asn Lys Glu Al - #a Leu Gln Ala Glu Val               300              - #   305              - #   310                           - - GGG CTC CCG GTG GAC CGG AAC ATC CCG CTG GT - #G GCG TTC ATC GGC AGG          3307                                                                        Gly Leu Pro Val Asp Arg Asn Ile Pro Leu Va - #l Ala Phe Ile Gly Arg            315                 3 - #20                 3 - #25                 3 -       #30                                                                               - - CTG GAA GAG CAG AAG GGC CCC GAC GTC ATG GC - #G GCC GCC ATC CCG         CAG     3355                                                                     Leu Glu Glu Gln Lys Gly Pro Asp Val Met Al - #a Ala Ala Ile Pro Gln                           335  - #               340  - #               345               - - CTC ATG GAG ATG GTG GAG GAC GTG CAG ATC GT - #T CTG CTG GTACGTGTGC           3404                                                                        Leu Met Glu Met Val Glu Asp Val Gln Ile Va - #l Leu Leu                                    350      - #           355                                          - - GCCGGCCGCC ACCCGGCTAC TACATGCGTG TATCGTTCGT TCTACTGGAA CA -              #TGCGTGTG   3464                                                                  - - AGCAACGCGA TGGATAATGC TGCAG GGC ACG GGC AAG AAG - #AAG TTC GAG CGC          3516                                                                                          - #          Gly Thr Gly Lys Lys - # Lys Phe Glu Arg                           - #          360       - #          365                       - - ATG CTC ATG AGC GCC GAG GAG AAG TTC CCA GG - #C AAG GTG CGC GCC GTG          3564                                                                        Met Leu Met Ser Ala Glu Glu Lys Phe Pro Gl - #y Lys Val Arg Ala Val                370              - #   375              - #   380                           - - GTC AAG TTC AAC GCG GCG CTG GCG CAC CAC AT - #C ATG GCC GGC GCC GAC          3612                                                                        Val Lys Phe Asn Ala Ala Leu Ala His His Il - #e Met Ala Gly Ala Asp            385                 3 - #90                 3 - #95                 4 -       #00                                                                               - - GTG CTC GCC GTC ACC AGC CGC TTC GAG CCC TG - #C GGC CTC ATC CAG         CTG     3660                                                                     Val Leu Ala Val Thr Ser Arg Phe Glu Pro Cy - #s Gly Leu Ile Gln Leu                           405  - #               410  - #               415               - - CAG GGG ATG CGA TAC GGA ACG GTACGAGAGA AAAAAAAAA - #T CCTGAATCCT             3711                                                                        Gln Gly Met Arg Tyr Gly Thr                                                                420                                                                 - - GACGAGAGGG ACAGAGACAG ATTATGAATG CTTCATCGAT TTGAATTGAT TG -              #ATCGATGT   3771                                                                  - - CTCCCGCTGC GACTCTTGCA G CCC TGC GCC TGC GCG TCC - #ACC GGT GGA CTC          3822                                                                                          - #      Pro Cys Ala Cys Ala Ser Thr - #Gly Gly Leu                            - #          425       - #          430                       - - GTC GAC ACC ATC ATC GAA GGC AAG ACC GGG TT - #C CAC ATG GGC CGC CTC          3870                                                                        Val Asp Thr Ile Ile Glu Gly Lys Thr Gly Ph - #e His Met Gly Arg Leu                435              - #   440              - #   445                           - - AGC GTC GAC GTAAGCCTAG CTCTGCCATG TTCTTTCTTC TTTCTTTCT - #G                  3919                                                                        Ser Val Asp                                                                    450                                                                             - - TATGTATGTA TGAATCAGCA CCGCCGTTCT TGTTTCGTCG TCGTCCTCTC TT - #CCCAG           3976                                                                         - - TGT AAC GTC GTG GAG CCG GCG GAC GTC AAG AA - #G GTG GCC ACC ACA TTG          4024                                                                        Cys Asn Val Val Glu Pro Ala Asp Val Lys Ly - #s Val Ala Thr Thr Leu                    455          - #       460          - #       465                       - - CAG CGC GCC ATC AAG GTG GTC GGC ACG CCG GC - #G TAC GAG GAG ATG GTG          4072                                                                        Gln Arg Ala Ile Lys Val Val Gly Thr Pro Al - #a Tyr Glu Glu Met Val                470              - #   475              - #   480                           - - AGG AAC TGC ATG ATC CAG GAT CTC TCC TGG AA - #G GTACGTACGC CCGCCCCGC     C   4125                                                                        Arg Asn Cys Met Ile Gln Asp Leu Ser Trp Ly - #s                                485                 4 - #90                 4 - #95                             - - CCGCCCCGCC AGAGCAGAGC GCCAAGATCG ACCGATCGAC CGACCACACG TA -              #CGCGCCTC   4185                                                                  - - GCTCCTGTCG CTGACCGTGG TTTAATTTGC GAAATGCGCA G GGC CCT - #GCC AAG            4238                                                                                          - #                  - #          Gly Pro Ala Lys             - - AAC TGG GAG AAC GTG CTG CTC AGC CTC GGG GT - #C GCC GGC GGC GAG CCA          4286                                                                        Asn Trp Glu Asn Val Leu Leu Ser Leu Gly Va - #l Ala Gly Gly Glu Pro            500                 5 - #05                 5 - #10                 5 -       #15                                                                               - - GGG GTC GAA GGC GAG GAG ATC GCG CCG CTC GC - #C AAG GAG AAC GTG         GCC     4334                                                                     Gly Val Glu Gly Glu Glu Ile Ala Pro Leu Al - #a Lys Glu Asn Val Ala                           520  - #               525  - #               530               - - GCG CCC TGA AGAGTTCGGC CTGCAGGGCC CCTGATCTCG CGCGTGGTG - #C                  4383                                                                        Ala Pro  *                                                                      - - AAAGATGTTG GGACATCTTC TTATATATGC TGTTTCGTTT ATGTGATATG GA -              #CAAGTATG   4443                                                                  - - TGTAGCTGCT TGCTTGTGCT AGTGTAATGT AGTGTAGTGG TGGCCAGTGG CA -             #CAACCTAA   4503                                                                  - - TAAGCGCATG AACTAATTGC TTGCGTGTGT AGTTAAGTAC CGATCGGTAA TT -             #TTATATTG   4563                                                                  - - CGAGTAAATA AATGGACCTG TAGTGGTGGA GTAAATAATC CCTGCTGTTC GG -             #TGTTCTTA   4623                                                                  - - TCGCTCCTCG TATAGATATT ATATAGAGTA CATTTTTCTC TCTCTGAATC CT -             #ACGTTTGT   4683                                                                  - - GAAATTTCTA TATCATTACT GTAAAATTTC TGCGTTCCAA AAGAGACCAT AG -             #CCTATCTT   4743                                                                  - - TGGCCCTGTT TGTTTCGGCT TCTGGCAGCT TCTGGCCACC AAAAGCTGCT GC - #GGACT           4800                                                                        - -  - - (2) INFORMATION FOR SEQ ID NO:5:                                      - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH:  533 ami - #no acids                                               (B) TYPE: amino acid                                                           (D) TOPOLOGY: linear                                                  - -     (ii) MOLECULE TYPE: protein                                            - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:5:                                - - Ala Ser Ala Gly Met Asn Val Val Phe Val Gl - #y Ala Glu Met Ala Pro         1               5 - #                 10 - #                 15               - - Trp Ser Lys Thr Gly Gly Leu Gly Asp Val Le - #u Gly Gly Leu Pro Pro                    20     - #             25     - #             30                   - - Ala Met Ala Ala Asn Gly His Arg Val Met Va - #l Val Ser Pro Arg Tyr                35         - #         40         - #         45                       - - Asp Gln Tyr Lys Asp Ala Trp Asp Thr Ser Va - #l Val Ser Glu Ile Lys            50             - #     55             - #     60                           - - Met Gly Asp Gly Tyr Glu Thr Val Arg Phe Ph - #e His Cys Tyr Lys Arg        65                 - # 70                 - # 75                 - # 80        - - Gly Val Asp Arg Val Phe Val Asp His Pro Le - #u Phe Leu Glu Arg Val                        85 - #                 90 - #                 95               - - Trp Gly Lys Thr Glu Glu Lys Ile Tyr Gly Pr - #o Val Ala Gly Thr Asp                   100      - #           105      - #           110                   - - Tyr Arg Asp Asn Gln Leu Arg Phe Ser Leu Le - #u Cys Gln Ala Ala Leu               115          - #       120          - #       125                       - - Glu Ala Pro Arg Ile Leu Ser Leu Asn Asn As - #n Pro Tyr Phe Ser Gly           130              - #   135              - #   140                           - - Pro Tyr Gly Glu Asp Val Val Phe Val Cys As - #n Asp Trp His Thr Gly       145                 1 - #50                 1 - #55                 1 -       #60                                                                               - - Pro Leu Ser Cys Tyr Leu Lys Ser Asn Tyr Gl - #n Ser His Gly Ile         Tyr                                                                                              165  - #               170  - #               175              - - Arg Asp Ala Lys Thr Ala Phe Cys Ile His As - #n Ile Ser Tyr Gln Gly                   180      - #           185      - #           190                   - - Arg Phe Ala Phe Ser Asp Tyr Pro Glu Leu As - #n Leu Pro Glu Arg Phe               195          - #       200          - #       205                       - - Lys Ser Ser Phe Asp Phe Ile Asp Gly Tyr Gl - #u Lys Pro Val Glu Gly           210              - #   215              - #   220                           - - Arg Lys Ile Asn Trp Met Lys Ala Gly Ile Le - #u Glu Ala Asp Arg Val       225                 2 - #30                 2 - #35                 2 -       #40                                                                               - - Leu Thr Val Ser Pro Tyr Tyr Ala Glu Glu Le - #u Ile Ser Gly Ile         Ala                                                                                              245  - #               250  - #               255              - - Arg Gly Cys Glu Leu Asp Asn Ile Met Arg Le - #u Thr Gly Ile Thr Gly                   260      - #           265      - #           270                   - - Ile Val Asn Gly Met Asp Val Ser Glu Trp As - #p Pro Ser Arg Asp Lys               275          - #       280          - #       285                       - - Tyr Ile Ala Val Lys Tyr Asp Val Ser Thr Al - #a Val Glu Ala Lys Ala           290              - #   295              - #   300                           - - Leu Asn Lys Glu Ala Leu Gln Ala Glu Val Gl - #y Leu Pro Val Asp Arg       305                 3 - #10                 3 - #15                 3 -       #20                                                                               - - Asn Ile Pro Leu Val Ala Phe Ile Gly Arg Le - #u Glu Glu Gln Lys         Gly                                                                                              325  - #               330  - #               335              - - Pro Asp Val Met Ala Ala Ala Ile Pro Gln Le - #u Met Glu Met Val Glu                   340      - #           345      - #           350                   - - Asp Val Gln Ile Val Leu Leu Gly Thr Gly Ly - #s Lys Lys Phe Glu Arg               355          - #       360          - #       365                       - - Met Leu Met Ser Ala Glu Glu Lys Phe Pro Gl - #y Lys Val Arg Ala Val           370              - #   375              - #   380                           - - Val Lys Phe Asn Ala Ala Leu Ala His His Il - #e Met Ala Gly Ala Asp       385                 3 - #90                 3 - #95                 4 -       #00                                                                               - - Val Leu Ala Val Thr Ser Arg Phe Glu Pro Cy - #s Gly Leu Ile Gln         Leu                                                                                              405  - #               410  - #               415              - - Gln Gly Met Arg Tyr Gly Thr Pro Cys Ala Cy - #s Ala Ser Thr Gly Gly                   420      - #           425      - #           430                   - - Leu Val Asp Thr Ile Ile Glu Gly Lys Thr Gl - #y Phe His Met Gly Arg               435          - #       440          - #       445                       - - Leu Ser Val Asp Cys Asn Val Val Glu Pro Al - #a Asp Val Lys Lys Val           450              - #   455              - #   460                           - - Ala Thr Thr Leu Gln Arg Ala Ile Lys Val Va - #l Gly Thr Pro Ala Tyr       465                 4 - #70                 4 - #75                 4 -       #80                                                                               - - Glu Glu Met Val Arg Asn Cys Met Ile Gln As - #p Leu Ser Trp Lys         Gly                                                                                              485  - #               490  - #               495              - - Pro Ala Lys Asn Trp Glu Asn Val Leu Leu Se - #r Leu Gly Val Ala Gly                   500      - #           505      - #           510                   - - Gly Glu Pro Gly Val Glu Gly Glu Glu Ile Al - #a Pro Leu Ala Lys Glu               515          - #       520          - #       525                       - - Asn Val Ala Ala Pro                                                           530                                                                         - -  - - (2) INFORMATION FOR SEQ ID NO:6:                                      - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 2542 base - #pairs                                                 (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: double                                                       (D) TOPOLOGY: Not Relev - #ant                                        - -     (ii) MOLECULE TYPE: cDNA to mRNA                                       - -    (iii) HYPOTHETICAL: NO                                                  - -     (vi) ORIGINAL SOURCE:                                                           (A) ORGANISM: Oryza sat - #iva                                        - -     (ix) FEATURE:                                                                   (A) NAME/KEY: CDS                                                              (B) LOCATION: 453..2282                                               - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:6:                                - - GAATTCAGTG TGAAGGAATA GATTCTCTTC AAAACAATTT AATCATTCAT CT -              #GATCTGCT     60                                                                  - - CAAAGCTCTG TGCATCTCCG GGTGCAACGG CCAGGATATT TATTGTGCAG TA -             #AAAAAATG    120                                                                  - - TCATATCCCC TAGCCACCCA AGAAACTGCT CCTTAAGTCC TTATAAGCAC AT -             #ATGGCATT    180                                                                  - - GTAATATATA TGTTTGAGTT TTAGCGACAA TTTTTTTAAA AACTTTTGGT CC -             #TTTTTATG    240                                                                  - - AACGTTTTAA GTTTCACTGT CTTTTTTTTT CGAATTTTAA ATGTAGCTTC AA -             #ATTCTAAT    300                                                                  - - CCCCAATCCA AATTGTAATA AACTTCAATT CTCCTAATTA ACATCTTAAT TC -             #ATTTATTT    360                                                                  - - GAAAACCAGT TCAAATTCTT TTTAGGCTCA CCAAACCTTA AACAATTCAA TT -             #CAGTGCAG    420                                                                  - - AGATCTTCCA CAGCAACAGC TAGACAACCA CC ATG TCG GCT CTC - # ACC ACG         TCC      473                                                                                       - #                  - #Met Ser Ala Leu Thr Thr Ser                           - #                  - #535                 - #540            - - CAG CTC GCC ACC TCG GCC ACC GGC TTC GGC AT - #C GCC GAC AGG TCG GCG           521                                                                        Gln Leu Ala Thr Ser Ala Thr Gly Phe Gly Il - #e Ala Asp Arg Ser Ala                        545      - #           550      - #           555                   - - CCG TCG TCG CTG CTC CGC CAC GGG TTC CAG GG - #C CTC AAG CCC CGC AGC           569                                                                        Pro Ser Ser Leu Leu Arg His Gly Phe Gln Gl - #y Leu Lys Pro Arg Ser                    560          - #       565          - #       570                       - - CCC GCC GGC GGC GAC GCG ACG TCG CTC AGC GT - #G ACG ACC AGC GCG CGC           617                                                                        Pro Ala Gly Gly Asp Ala Thr Ser Leu Ser Va - #l Thr Thr Ser Ala Arg                575              - #   580              - #   585                           - - GCG ACG CCC AAG CAG CAG CGG TCG GTG CAG CG - #T GGC AGC CGG AGG TTC           665                                                                        Ala Thr Pro Lys Gln Gln Arg Ser Val Gln Ar - #g Gly Ser Arg Arg Phe            590                 5 - #95                 6 - #00                 6 -       #05                                                                               - - CCC TCC GTC GTC GTG TAC GCC ACC GGC GCC GG - #C ATG AAC GTC GTG         TTC      713                                                                     Pro Ser Val Val Val Tyr Ala Thr Gly Ala Gl - #y Met Asn Val Val Phe                           610  - #               615  - #               620               - - GTC GGC GCC GAG ATG GCC CCC TGG AGC AAG AC - #C GGC GGC CTC GGT GAC           761                                                                        Val Gly Ala Glu Met Ala Pro Trp Ser Lys Th - #r Gly Gly Leu Gly Asp                        625      - #           630      - #           635                   - - GTC CTC GGT GGC CTC CCC CCT GCC ATG GCT GC - #G AAT GGC CAC AGG GTC           809                                                                        Val Leu Gly Gly Leu Pro Pro Ala Met Ala Al - #a Asn Gly His Arg Val                    640          - #       645          - #       650                       - - ATG GTG ATC TCT CCT CGG TAC GAC CAG TAC AA - #G GAC GCT TGG GAT ACC           857                                                                        Met Val Ile Ser Pro Arg Tyr Asp Gln Tyr Ly - #s Asp Ala Trp Asp Thr                655              - #   660              - #   665                           - - AGC GTT GTG GCT GAG ATC AAG GTT GCA GAC AG - #G TAC GAG AGG GTG AGG           905                                                                        Ser Val Val Ala Glu Ile Lys Val Ala Asp Ar - #g Tyr Glu Arg Val Arg            670                 6 - #75                 6 - #80                 6 -       #85                                                                               - - TTT TTC CAT TGC TAC AAG CGT GGA GTC GAC CG - #T GTG TTC ATC GAC         CAT      953                                                                     Phe Phe His Cys Tyr Lys Arg Gly Val Asp Ar - #g Val Phe Ile Asp His                           690  - #               695  - #               700               - - CCG TCA TTC CTG GAG AAG GTT TGG GGA AAG AC - #C GGT GAG AAG ATC TAC          1001                                                                        Pro Ser Phe Leu Glu Lys Val Trp Gly Lys Th - #r Gly Glu Lys Ile Tyr                        705      - #           710      - #           715                   - - GGA CCT GAC ACT GGA GTT GAT TAC AAA GAC AA - #C CAG ATG CGT TTC AGC          1049                                                                        Gly Pro Asp Thr Gly Val Asp Tyr Lys Asp As - #n Gln Met Arg Phe Ser                    720          - #       725          - #       730                       - - CTT CTT TGC CAG GCA GCA CTC GAG GCT CCT AG - #G ATC CTA AAC CTC AAC          1097                                                                        Leu Leu Cys Gln Ala Ala Leu Glu Ala Pro Ar - #g Ile Leu Asn Leu Asn                735              - #   740              - #   745                           - - AAC AAC CCA TAC TTC AAA GGA ACT TAT GGT GA - #G GAT GTT GTG TTC GTC          1145                                                                        Asn Asn Pro Tyr Phe Lys Gly Thr Tyr Gly Gl - #u Asp Val Val Phe Val            750                 7 - #55                 7 - #60                 7 -       #65                                                                               - - TGC AAC GAC TGG CAC ACT GGC CCA CTG GCG AG - #C TAC CTG AAG AAC         AAC     1193                                                                     Cys Asn Asp Trp His Thr Gly Pro Leu Ala Se - #r Tyr Leu Lys Asn Asn                           770  - #               775  - #               780               - - TAC CAG CCC AAT GGC ATC TAC AGG AAT GCA AA - #G GTT GCT TTC TGC ATC          1241                                                                        Tyr Gln Pro Asn Gly Ile Tyr Arg Asn Ala Ly - #s Val Ala Phe Cys Ile                        785      - #           790      - #           795                   - - CAC AAC ATC TCC TAC CAG GGC CGT TTC GCT TT - #C GAG GAT TAC CCT GAG          1289                                                                        His Asn Ile Ser Tyr Gln Gly Arg Phe Ala Ph - #e Glu Asp Tyr Pro Glu                    800          - #       805          - #       810                       - - CTG AAC CTC TCC GAG AGG TTC AGG TCA TCC TT - #C GAT TTC ATC GAC GGG          1337                                                                        Leu Asn Leu Ser Glu Arg Phe Arg Ser Ser Ph - #e Asp Phe Ile Asp Gly                815              - #   820              - #   825                           - - TAT GAC ACG CCG GTG GAG GGC AGG AAG ATC AA - #C TGG ATG AAG GCC GGA          1385                                                                        Tyr Asp Thr Pro Val Glu Gly Arg Lys Ile As - #n Trp Met Lys Ala Gly            830                 8 - #35                 8 - #40                 8 -       #45                                                                               - - ATC CTG GAA GCC GAC AGG GTG CTC ACC GTG AG - #C CCG TAC TAC GCC         GAG     1433                                                                     Ile Leu Glu Ala Asp Arg Val Leu Thr Val Se - #r Pro Tyr Tyr Ala Glu                           850  - #               855  - #               860               - - GAG CTC ATC TCC GGC ATC GCC AGG GGA TGC GA - #G CTC GAC AAC ATC ATG          1481                                                                        Glu Leu Ile Ser Gly Ile Ala Arg Gly Cys Gl - #u Leu Asp Asn Ile Met                        865      - #           870      - #           875                   - - CGG CTC ACC GGC ATC ACC GGC ATC GTC AAC GG - #C ATG GAC GTC AGC GAG          1529                                                                        Arg Leu Thr Gly Ile Thr Gly Ile Val Asn Gl - #y Met Asp Val Ser Glu                    880          - #       885          - #       890                       - - TGG GAT CCT AGC AAG GAC AAG TAC ATC ACC GC - #C AAG TAC GAC GCA ACC          1577                                                                        Trp Asp Pro Ser Lys Asp Lys Tyr Ile Thr Al - #a Lys Tyr Asp Ala Thr                895              - #   900              - #   905                           - - ACG GCA ATC GAG GCG AAG GCG CTG AAC AAG GA - #G GCG TTG CAG GCG GAG          1625                                                                        Thr Ala Ile Glu Ala Lys Ala Leu Asn Lys Gl - #u Ala Leu Gln Ala Glu            910                 9 - #15                 9 - #20                 9 -       #25                                                                               - - GCG GGT CTT CCG GTC GAC AGG AAA ATC CCA CT - #G ATC GCG TTC ATC         GGC     1673                                                                     Ala Gly Leu Pro Val Asp Arg Lys Ile Pro Le - #u Ile Ala Phe Ile Gly                           930  - #               935  - #               940               - - AGG CTG GAG GAA CAG AAG GGC CCT GAC GTC AT - #G GCC GCC GCC ATC CCG          1721                                                                        Arg Leu Glu Glu Gln Lys Gly Pro Asp Val Me - #t Ala Ala Ala Ile Pro                        945      - #           950      - #           955                   - - GAG CTC ATG CAG GAG GAC GTC CAG ATC GTT CT - #T CTG GGT ACT GGA AAG          1769                                                                        Glu Leu Met Gln Glu Asp Val Gln Ile Val Le - #u Leu Gly Thr Gly Lys                    960          - #       965          - #       970                       - - AAG AAG TTC GAG AAG CTG CTC AAG AGC ATG GA - #G GAG AAG TAT CCG GGC          1817                                                                        Lys Lys Phe Glu Lys Leu Leu Lys Ser Met Gl - #u Glu Lys Tyr Pro Gly                975              - #   980              - #   985                           - - AAG GTG AGG GCG GTG GTG AAG TTC AAC GCG CC - #G CTT GCT CAT CTC ATC          1865                                                                        Lys Val Arg Ala Val Val Lys Phe Asn Ala Pr - #o Leu Ala His Leu Ile            990                 9 - #95                 1 - #000                1005        - - ATG GCC GGA GCC GAC GTG CTC GCC GTC CCC AG - #C CGC TTC GAG CCC TGT          1913                                                                        Met Ala Gly Ala Asp Val Leu Ala Val Pro Se - #r Arg Phe Glu Pro Cys                            1010 - #               1015  - #              1020              - - GGA CTC ATC CAG CTG CAG GGG ATG AGA TAC GG - #A ACG CCC TGT GCT TGC          1961                                                                        Gly Leu Ile Gln Leu Gln Gly Met Arg Tyr Gl - #y Thr Pro Cys Ala Cys                        1025     - #           1030      - #          1035                  - - GCG TCC ACC GGT GGG CTC GTG GAC ACG GTC AT - #C GAA GGC AAG ACT GGT          2009                                                                        Ala Ser Thr Gly Gly Leu Val Asp Thr Val Il - #e Glu Gly Lys Thr Gly                    1040         - #       1045          - #      1050                      - - TTC CAC ATG GGC CGT CTC AGC GTC GAC TGC AA - #G GTG GTG GAG CCA AGC          2057                                                                        Phe His Met Gly Arg Leu Ser Val Asp Cys Ly - #s Val Val Glu Pro Ser                1055             - #   1060              - #  1065                          - - GAC GTG AAG AAG GTG GCG GCC ACC CTG AAG CG - #C GCC ATC AAG GTC GTC          2105                                                                        Asp Val Lys Lys Val Ala Ala Thr Leu Lys Ar - #g Ala Ile Lys Val Val            1070                1075 - #                1080 - #               1085         - - GGC ACG CCG GCG TAC GAG GAG ATG GTC AGG AA - #C TGC ATG AAC CAG GAC          2153                                                                        Gly Thr Pro Ala Tyr Glu Glu Met Val Arg As - #n Cys Met Asn Gln Asp                            1090 - #               1095  - #              1100              - - CTC TCC TGG AAG GGG CCT GCG AAG AAC TGG GA - #G AAT GTG CTC CTG GGC          2201                                                                        Leu Ser Trp Lys Gly Pro Ala Lys Asn Trp Gl - #u Asn Val Leu Leu Gly                        1105     - #           1110      - #          1115                  - - CTG GGC GTC GCC GGC AGC GCG CCG GGG ATC GA - #A GGC GAC GAG ATC GCG          2249                                                                        Leu Gly Val Ala Gly Ser Ala Pro Gly Ile Gl - #u Gly Asp Glu Ile Ala                    1120         - #       1125          - #      1130                      - - CCG CTC GCC AAG GAG AAC GTG GCT GCT CCT TG - #A AGAGCCTGAG ATCTACATA     T   2302                                                                        Pro Leu Ala Lys Glu Asn Val Ala Ala Pro  - #*                                      1135             - #   1140                                                 - - GGAGTGATTA ATTAATATAG CAGTATATGG ATGAGAGACG AATGAACCAG TG -              #GTTTGTTT   2362                                                                  - - GTTGTAGTGA ATTTGTAGCT ATAGCCAATT ATATAGGCTA ATAAGTTTGA TG -             #TTGTACTC   2422                                                                  - - TTCTGGGTGT GCTTAAGTAT CTTATCGGAC CCTGAATTTA TGTGTGTGGC TT -             #ATTGCCAA   2482                                                                  - - TAATATTAAG TAATAAAGGG TTTATTATAT TATTATATAT GTTATATTAT AC -             #TAAAAAAA   2542                                                                  - -  - - (2) INFORMATION FOR SEQ ID NO:7:                                      - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH:  609 ami - #no acids                                               (B) TYPE: amino acid                                                           (D) TOPOLOGY: linear                                                  - -     (ii) MOLECULE TYPE: protein                                            - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:7:                                - - Met Ser Ala Leu Thr Thr Ser Gln Leu Ala Th - #r Ser Ala Thr Gly         Phe                                                                                1               5 - #                 10 - #                 15              - - Gly Ile Ala Asp Arg Ser Ala Pro Ser Ser Le - #u Leu Arg His Gly Phe                    20     - #             25     - #             30                   - - Gln Gly Leu Lys Pro Arg Ser Pro Ala Gly Gl - #y Asp Ala Thr Ser Leu                35         - #         40         - #         45                       - - Ser Val Thr Thr Ser Ala Arg Ala Thr Pro Ly - #s Gln Gln Arg Ser Val            50             - #     55             - #     60                           - - Gln Arg Gly Ser Arg Arg Phe Pro Ser Val Va - #l Val Tyr Ala Thr Gly        65                 - # 70                 - # 75                 - # 80        - - Ala Gly Met Asn Val Val Phe Val Gly Ala Gl - #u Met Ala Pro Trp Ser                        85 - #                 90 - #                 95               - - Lys Thr Gly Gly Leu Gly Asp Val Leu Gly Gl - #y Leu Pro Pro Ala Met                   100      - #           105      - #           110                   - - Ala Ala Asn Gly His Arg Val Met Val Ile Se - #r Pro Arg Tyr Asp Gln               115          - #       120          - #       125                       - - Tyr Lys Asp Ala Trp Asp Thr Ser Val Val Al - #a Glu Ile Lys Val Ala           130              - #   135              - #   140                           - - Asp Arg Tyr Glu Arg Val Arg Phe Phe His Cy - #s Tyr Lys Arg Gly Val       145                 1 - #50                 1 - #55                 1 -       #60                                                                               - - Asp Arg Val Phe Ile Asp His Pro Ser Phe Le - #u Glu Lys Val Trp         Gly                                                                                              165  - #               170  - #               175              - - Lys Thr Gly Glu Lys Ile Tyr Gly Pro Asp Th - #r Gly Val Asp Tyr Lys                   180      - #           185      - #           190                   - - Asp Asn Gln Met Arg Phe Ser Leu Leu Cys Gl - #n Ala Ala Leu Glu Ala               195          - #       200          - #       205                       - - Pro Arg Ile Leu Asn Leu Asn Asn Asn Pro Ty - #r Phe Lys Gly Thr Tyr           210              - #   215              - #   220                           - - Gly Glu Asp Val Val Phe Val Cys Asn Asp Tr - #p His Thr Gly Pro Leu       225                 2 - #30                 2 - #35                 2 -       #40                                                                               - - Ala Ser Tyr Leu Lys Asn Asn Tyr Gln Pro As - #n Gly Ile Tyr Arg         Asn                                                                                              245  - #               250  - #               255              - - Ala Lys Val Ala Phe Cys Ile His Asn Ile Se - #r Tyr Gln Gly Arg Phe                   260      - #           265      - #           270                   - - Ala Phe Glu Asp Tyr Pro Glu Leu Asn Leu Se - #r Glu Arg Phe Arg Ser               275          - #       280          - #       285                       - - Ser Phe Asp Phe Ile Asp Gly Tyr Asp Thr Pr - #o Val Glu Gly Arg Lys           290              - #   295              - #   300                           - - Ile Asn Trp Met Lys Ala Gly Ile Leu Glu Al - #a Asp Arg Val Leu Thr       305                 3 - #10                 3 - #15                 3 -       #20                                                                               - - Val Ser Pro Tyr Tyr Ala Glu Glu Leu Ile Se - #r Gly Ile Ala Arg         Gly                                                                                              325  - #               330  - #               335              - - Cys Glu Leu Asp Asn Ile Met Arg Leu Thr Gl - #y Ile Thr Gly Ile Val                   340      - #           345      - #           350                   - - Asn Gly Met Asp Val Ser Glu Trp Asp Pro Se - #r Lys Asp Lys Tyr Ile               355          - #       360          - #       365                       - - Thr Ala Lys Tyr Asp Ala Thr Thr Ala Ile Gl - #u Ala Lys Ala Leu Asn           370              - #   375              - #   380                           - - Lys Glu Ala Leu Gln Ala Glu Ala Gly Leu Pr - #o Val Asp Arg Lys Ile       385                 3 - #90                 3 - #95                 4 -       #00                                                                               - - Pro Leu Ile Ala Phe Ile Gly Arg Leu Glu Gl - #u Gln Lys Gly Pro         Asp                                                                                              405  - #               410  - #               415              - - Val Met Ala Ala Ala Ile Pro Glu Leu Met Gl - #n Glu Asp Val Gln Ile                   420      - #           425      - #           430                   - - Val Leu Leu Gly Thr Gly Lys Lys Lys Phe Gl - #u Lys Leu Leu Lys Ser               435          - #       440          - #       445                       - - Met Glu Glu Lys Tyr Pro Gly Lys Val Arg Al - #a Val Val Lys Phe Asn           450              - #   455              - #   460                           - - Ala Pro Leu Ala His Leu Ile Met Ala Gly Al - #a Asp Val Leu Ala Val       465                 4 - #70                 4 - #75                 4 -       #80                                                                               - - Pro Ser Arg Phe Glu Pro Cys Gly Leu Ile Gl - #n Leu Gln Gly Met         Arg                                                                                              485  - #               490  - #               495              - - Tyr Gly Thr Pro Cys Ala Cys Ala Ser Thr Gl - #y Gly Leu Val Asp Thr                   500      - #           505      - #           510                   - - Val Ile Glu Gly Lys Thr Gly Phe His Met Gl - #y Arg Leu Ser Val Asp               515          - #       520          - #       525                       - - Cys Lys Val Val Glu Pro Ser Asp Val Lys Ly - #s Val Ala Ala Thr Leu           530              - #   535              - #   540                           - - Lys Arg Ala Ile Lys Val Val Gly Thr Pro Al - #a Tyr Glu Glu Met Val       545                 5 - #50                 5 - #55                 5 -       #60                                                                               - - Arg Asn Cys Met Asn Gln Asp Leu Ser Trp Ly - #s Gly Pro Ala Lys         Asn                                                                                              565  - #               570  - #               575              - - Trp Glu Asn Val Leu Leu Gly Leu Gly Val Al - #a Gly Ser Ala Pro Gly                   580      - #           585      - #           590                   - - Ile Glu Gly Asp Glu Ile Ala Pro Leu Ala Ly - #s Glu Asn Val Ala Ala               595          - #       600          - #       605                       - - Pro                                                                           610                                                                         - -  - - (2) INFORMATION FOR SEQ ID NO:8:                                      - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 2007 base - #pairs                                                 (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: double                                                       (D) TOPOLOGY: Not Relev - #ant                                        - -     (ii) MOLECULE TYPE: cDNA to mRNA                                       - -    (iii) HYPOTHETICAL: NO                                                  - -     (vi) ORIGINAL SOURCE:                                                           (A) ORGANISM: Zea mays                                                - -     (ix) FEATURE:                                                                   (A) NAME/KEY: CDS                                                              (B) LOCATION: 1..2007                                                 - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:8:                                - - GCT GAG GCT GAG GCC GGG GGC AAG GAC GCG CC - #G CCG GAG AGG AGC GGC            48                                                                        Ala Glu Ala Glu Ala Gly Gly Lys Asp Ala Pr - #o Pro Glu Arg Ser Gly                            615  - #               620  - #               625               - - GAC GCC GCC AGG TTG CCC CGC GCT CGG CGC AA - #T GCG GTC TCC AAA CGG            96                                                                        Asp Ala Ala Arg Leu Pro Arg Ala Arg Arg As - #n Ala Val Ser Lys Arg                        630      - #           635      - #           640                   - - AGG GAT CCT CTT CAG CCG GTC GGC CGG TAC GG - #C TCC GCG ACG GGA AAC           144                                                                        Arg Asp Pro Leu Gln Pro Val Gly Arg Tyr Gl - #y Ser Ala Thr Gly Asn                    645          - #       650          - #       655                       - - ACG GCC AGG ACC GGC GCC GCG TCC TGC CAG AA - #C GCC GCA TTG GCG GAC           192                                                                        Thr Ala Arg Thr Gly Ala Ala Ser Cys Gln As - #n Ala Ala Leu Ala Asp                660              - #   665              - #   670                           - - GTT GAG ATC GTT GAG ATC AAG TCC ATC GTC GC - #C GCG CCG CCG ACG AGC           240                                                                        Val Glu Ile Val Glu Ile Lys Ser Ile Val Al - #a Ala Pro Pro Thr Ser            675                 6 - #80                 6 - #85                 6 -       #90                                                                               - - ATA GTG AAG TTC CCA GGG CGC GGG CTA CAG GA - #T GAT CCT TCC CTC         TGG      288                                                                     Ile Val Lys Phe Pro Gly Arg Gly Leu Gln As - #p Asp Pro Ser Leu Trp                           695  - #               700  - #               705               - - GAC ATA GCA CCG GAG ACT GTC CTC CCA GCC CC - #G AAG CCA CTG CAT GAA           336                                                                        Asp Ile Ala Pro Glu Thr Val Leu Pro Ala Pr - #o Lys Pro Leu His Glu                        710      - #           715      - #           720                   - - TCG CCT GCG GTT GAC GGA GAT TCA AAT GGA AT - #T GCA CCT CCT ACA GTT           384                                                                        Ser Pro Ala Val Asp Gly Asp Ser Asn Gly Il - #e Ala Pro Pro Thr Val                    725          - #       730          - #       735                       - - GAG CCA TTA GTA CAG GAG GCC ACT TGG GAT TT - #C AAG AAA TAC ATC GGT           432                                                                        Glu Pro Leu Val Gln Glu Ala Thr Trp Asp Ph - #e Lys Lys Tyr Ile Gly                740              - #   745              - #   750                           - - TTT GAC GAG CCT GAC GAA GCG AAG GAT GAT TC - #C AGG GTT GGT GCA GAT           480                                                                        Phe Asp Glu Pro Asp Glu Ala Lys Asp Asp Se - #r Arg Val Gly Ala Asp            755                 7 - #60                 7 - #65                 7 -       #70                                                                               - - GAT GCT GGT TCT TTT GAA CAT TAT GGG ACA AT - #G ATT CTG GGC CTT         TGT      528                                                                     Asp Ala Gly Ser Phe Glu His Tyr Gly Thr Me - #t Ile Leu Gly Leu Cys                           775  - #               780  - #               785               - - GGG GAG AAT GTT ATG AAC GTG ATC GTG GTG GC - #T GCT GAA TGT TCT CCA           576                                                                        Gly Glu Asn Val Met Asn Val Ile Val Val Al - #a Ala Glu Cys Ser Pro                        790      - #           795      - #           800                   - - TGG TGC AAA ACA GGT GGT CTT GGA GAT GTT GT - #G GGA GCT TTA CCC AAG           624                                                                        Trp Cys Lys Thr Gly Gly Leu Gly Asp Val Va - #l Gly Ala Leu Pro Lys                    805          - #       810          - #       815                       - - GCT TTA GCG AGA AGA GGA CAT CGT GTT ATG GT - #T GTG GTA CCA AGG TAT           672                                                                        Ala Leu Ala Arg Arg Gly His Arg Val Met Va - #l Val Val Pro Arg Tyr                820              - #   825              - #   830                           - - GGG GAC TAT GTG GAA GCC TTT GAT ATG GGA AT - #C CGG AAA TAC TAC AAA           720                                                                        Gly Asp Tyr Val Glu Ala Phe Asp Met Gly Il - #e Arg Lys Tyr Tyr Lys            835                 8 - #40                 8 - #45                 8 -       #50                                                                               - - GCT GCA GGA CAG GAC CTA GAA GTG AAC TAT TT - #C CAT GCA TTT ATT         GAT      768                                                                     Ala Ala Gly Gln Asp Leu Glu Val Asn Tyr Ph - #e His Ala Phe Ile Asp                           855  - #               860  - #               865               - - GGA GTC GAC TTT GTG TTC ATT GAT GCC TCT TT - #C CGG CAC CGT CAA GAT           816                                                                        Gly Val Asp Phe Val Phe Ile Asp Ala Ser Ph - #e Arg His Arg Gln Asp                        870      - #           875      - #           880                   - - GAC ATA TAT GGG GGA AGT AGG CAG GAA ATC AT - #G AAG CGC ATG ATT TTG           864                                                                        Asp Ile Tyr Gly Gly Ser Arg Gln Glu Ile Me - #t Lys Arg Met Ile Leu                    885          - #       890          - #       895                       - - TTT TGC AAG GTT GCT GTT GAG GTT CCT TGG CA - #C GTT CCA TGC GGT GGT           912                                                                        Phe Cys Lys Val Ala Val Glu Val Pro Trp Hi - #s Val Pro Cys Gly Gly                900              - #   905              - #   910                           - - GTG TGC TAC GGA GAT GGA AAT TTG GTG TTC AT - #T GCC ATG AAT TGG CAC           960                                                                        Val Cys Tyr Gly Asp Gly Asn Leu Val Phe Il - #e Ala Met Asn Trp His            915                 9 - #20                 9 - #25                 9 -       #30                                                                               - - ACT GCA CTC CTG CCT GTT TAT CTG AAG GCA TA - #T TAC AGA GAC CAT         GGG     1008                                                                     Thr Ala Leu Leu Pro Val Tyr Leu Lys Ala Ty - #r Tyr Arg Asp His Gly                           935  - #               940  - #               945               - - TTA ATG CAG TAC ACT CGC TCC GTC CTC GTC AT - #A CAT AAC ATC GGC CAC          1056                                                                        Leu Met Gln Tyr Thr Arg Ser Val Leu Val Il - #e His Asn Ile Gly His                        950      - #           955      - #           960                   - - CAG GGC CGT GGT CCT GTA CAT GAA TTC CCG TA - #C ATG GAC TTG CTG AAC          1104                                                                        Gln Gly Arg Gly Pro Val His Glu Phe Pro Ty - #r Met Asp Leu Leu Asn                    965          - #       970          - #       975                       - - ACT AAC CTT CAA CAT TTC GAG CTG TAC GAT CC - #C GTC GGT GGC GAG CAC          1152                                                                        Thr Asn Leu Gln His Phe Glu Leu Tyr Asp Pr - #o Val Gly Gly Glu His                980              - #   985              - #   990                           - - GCC AAC ATC TTT GCC GCG TGT GTT CTG AAG AT - #G GCA GAC CGG GTG GTG          1200                                                                        Ala Asn Ile Phe Ala Ala Cys Val Leu Lys Me - #t Ala Asp Arg Val Val            995                 1 - #000                1005 - #                1010        - - ACT GTC AGC CGC GGC TAC CTG TGG GAG CTG AA - #G ACA GTG GAA GGC GGC          1248                                                                        Thr Val Ser Arg Gly Tyr Leu Trp Glu Leu Ly - #s Thr Val Glu Gly Gly                            1015 - #               1020  - #              1025              - - TGG GGC CTC CAC GAC ATC ATC CGT TCT AAC GA - #C TGG AAG ATC AAT GGC          1296                                                                        Trp Gly Leu His Asp Ile Ile Arg Ser Asn As - #p Trp Lys Ile Asn Gly                        1030     - #           1035      - #          1040                  - - ATT CGT GAA CGC ATC GAC CAC CAG GAG TGG AA - #C CCC AAG GTG GAC GTG          1344                                                                        Ile Arg Glu Arg Ile Asp His Gln Glu Trp As - #n Pro Lys Val Asp Val                    1045         - #       1050          - #      1055                      - - CAC CTG CGG TCG GAC GGC TAC ACC AAC TAC TC - #C CTC GAG ACA CTC GAC          1392                                                                        His Leu Arg Ser Asp Gly Tyr Thr Asn Tyr Se - #r Leu Glu Thr Leu Asp                1060             - #   1065              - #  1070                          - - GCT GGA AAG CGG CAG TGC AAG GCG GCC CTG CA - #G CGG GAC GTG GGC CTG          1440                                                                        Ala Gly Lys Arg Gln Cys Lys Ala Ala Leu Gl - #n Arg Asp Val Gly Leu            1075                1080 - #                1085 - #               1090         - - GAA GTG CGC GAC GAC GTG CCG CTG CTC GGC TT - #C ATC GGG CGT CTG GAT          1488                                                                        Glu Val Arg Asp Asp Val Pro Leu Leu Gly Ph - #e Ile Gly Arg Leu Asp                            1095 - #               1100  - #              1105              - - GGA CAG AAG GGC GTG GAC ATC ATC GGG GAC GC - #G ATG CCG TGG ATC GCG          1536                                                                        Gly Gln Lys Gly Val Asp Ile Ile Gly Asp Al - #a Met Pro Trp Ile Ala                        1110     - #           1115      - #          1120                  - - GGG CAG GAC GTG CAG CTG GTG ATG CTG GGC AC - #C GGC CCA CCT GAC CTG          1584                                                                        Gly Gln Asp Val Gln Leu Val Met Leu Gly Th - #r Gly Pro Pro Asp Leu                    1125         - #       1130          - #      1135                      - - GAA CGA ATG CTG CAG CAC TTG GAG CGG GAG CA - #T CCC AAC AAG GTG CGC          1632                                                                        Glu Arg Met Leu Gln His Leu Glu Arg Glu Hi - #s Pro Asn Lys Val Arg                1140             - #   1145              - #  1150                          - - GGG TGG GTC GGG TTC TCG GTC CTA ATG GTG CA - #T CGC ATC ACG CCG GGC          1680                                                                        Gly Trp Val Gly Phe Ser Val Leu Met Val Hi - #s Arg Ile Thr Pro Gly            1155                1160 - #                1165 - #               1170         - - GCC AGC GTG CTG GTG ATG CCC TCC CGC TTC GC - #C GGC GGG CTG AAC CAG          1728                                                                        Ala Ser Val Leu Val Met Pro Ser Arg Phe Al - #a Gly Gly Leu Asn Gln                            1175 - #               1180  - #              1185              - - CTC TAC GCG ATG GCA TAC GGC ACC GTC CCT GT - #G GTG CAC GCC GTG GGC          1776                                                                        Leu Tyr Ala Met Ala Tyr Gly Thr Val Pro Va - #l Val His Ala Val Gly                        1190     - #           1195      - #          1200                  - - GGG CTC AGG GAC ACC GTG GCG CCG TTC GAC CC - #G TTC GGC GAC GCC GGG          1824                                                                        Gly Leu Arg Asp Thr Val Ala Pro Phe Asp Pr - #o Phe Gly Asp Ala Gly                    1205         - #       1210          - #      1215                      - - CTC GGG TGG ACT TTT GAC CGC GCC GAG GCC AA - #C AAG CTG ATC GAG GTG          1872                                                                        Leu Gly Trp Thr Phe Asp Arg Ala Glu Ala As - #n Lys Leu Ile Glu Val                1220             - #   1225              - #  1230                          - - CTC AGC CAC TGC CTC GAC ACG TAC CGA AAC TA - #C GAG GAG AGC TGG AAG          1920                                                                        Leu Ser His Cys Leu Asp Thr Tyr Arg Asn Ty - #r Glu Glu Ser Trp Lys            1235                1240 - #                1245 - #               1250         - - AGT CTC CAG GCG CGC GGC ATG TCG CAG AAC CT - #C AGC TGG GAC CAC GCG          1968                                                                        Ser Leu Gln Ala Arg Gly Met Ser Gln Asn Le - #u Ser Trp Asp His Ala                            1255 - #               1260  - #              1265              - - GCT GAG CTC TAC GAG GAC GTC CTT GTC AAG TA - #C CAG TGG                   - #   2007                                                                     Ala Glu Leu Tyr Glu Asp Val Leu Val Lys Ty - #r Gln Trp                                    1270     - #           1275                                         - -  - - (2) INFORMATION FOR SEQ ID NO:9:                                      - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 669 amino - #acids                                                 (B) TYPE: amino acid                                                           (D) TOPOLOGY: linear                                                  - -     (ii) MOLECULE TYPE: protein                                            - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:9:                                - - Ala Glu Ala Glu Ala Gly Gly Lys Asp Ala Pr - #o Pro Glu Arg Ser Gly         1               5 - #                 10 - #                 15               - - Asp Ala Ala Arg Leu Pro Arg Ala Arg Arg As - #n Ala Val Ser Lys Arg                    20     - #             25     - #             30                   - - Arg Asp Pro Leu Gln Pro Val Gly Arg Tyr Gl - #y Ser Ala Thr Gly Asn                35         - #         40         - #         45                       - - Thr Ala Arg Thr Gly Ala Ala Ser Cys Gln As - #n Ala Ala Leu Ala Asp            50             - #     55             - #     60                           - - Val Glu Ile Val Glu Ile Lys Ser Ile Val Al - #a Ala Pro Pro Thr Ser        65                 - # 70                 - # 75                 - # 80        - - Ile Val Lys Phe Pro Gly Arg Gly Leu Gln As - #p Asp Pro Ser Leu Trp                        85 - #                 90 - #                 95               - - Asp Ile Ala Pro Glu Thr Val Leu Pro Ala Pr - #o Lys Pro Leu His Glu                   100      - #           105      - #           110                   - - Ser Pro Ala Val Asp Gly Asp Ser Asn Gly Il - #e Ala Pro Pro Thr Val               115          - #       120          - #       125                       - - Glu Pro Leu Val Gln Glu Ala Thr Trp Asp Ph - #e Lys Lys Tyr Ile Gly           130              - #   135              - #   140                           - - Phe Asp Glu Pro Asp Glu Ala Lys Asp Asp Se - #r Arg Val Gly Ala Asp       145                 1 - #50                 1 - #55                 1 -       #60                                                                               - - Asp Ala Gly Ser Phe Glu His Tyr Gly Thr Me - #t Ile Leu Gly Leu         Cys                                                                                              165  - #               170  - #               175              - - Gly Glu Asn Val Met Asn Val Ile Val Val Al - #a Ala Glu Cys Ser Pro                   180      - #           185      - #           190                   - - Trp Cys Lys Thr Gly Gly Leu Gly Asp Val Va - #l Gly Ala Leu Pro Lys               195          - #       200          - #       205                       - - Ala Leu Ala Arg Arg Gly His Arg Val Met Va - #l Val Val Pro Arg Tyr           210              - #   215              - #   220                           - - Gly Asp Tyr Val Glu Ala Phe Asp Met Gly Il - #e Arg Lys Tyr Tyr Lys       225                 2 - #30                 2 - #35                 2 -       #40                                                                               - - Ala Ala Gly Gln Asp Leu Glu Val Asn Tyr Ph - #e His Ala Phe Ile         Asp                                                                                              245  - #               250  - #               255              - - Gly Val Asp Phe Val Phe Ile Asp Ala Ser Ph - #e Arg His Arg Gln Asp                   260      - #           265      - #           270                   - - Asp Ile Tyr Gly Gly Ser Arg Gln Glu Ile Me - #t Lys Arg Met Ile Leu               275          - #       280          - #       285                       - - Phe Cys Lys Val Ala Val Glu Val Pro Trp Hi - #s Val Pro Cys Gly Gly           290              - #   295              - #   300                           - - Val Cys Tyr Gly Asp Gly Asn Leu Val Phe Il - #e Ala Met Asn Trp His       305                 3 - #10                 3 - #15                 3 -       #20                                                                               - - Thr Ala Leu Leu Pro Val Tyr Leu Lys Ala Ty - #r Tyr Arg Asp His         Gly                                                                                              325  - #               330  - #               335              - - Leu Met Gln Tyr Thr Arg Ser Val Leu Val Il - #e His Asn Ile Gly His                   340      - #           345      - #           350                   - - Gln Gly Arg Gly Pro Val His Glu Phe Pro Ty - #r Met Asp Leu Leu Asn               355          - #       360          - #       365                       - - Thr Asn Leu Gln His Phe Glu Leu Tyr Asp Pr - #o Val Gly Gly Glu His           370              - #   375              - #   380                           - - Ala Asn Ile Phe Ala Ala Cys Val Leu Lys Me - #t Ala Asp Arg Val Val       385                 3 - #90                 3 - #95                 4 -       #00                                                                               - - Thr Val Ser Arg Gly Tyr Leu Trp Glu Leu Ly - #s Thr Val Glu Gly         Gly                                                                                              405  - #               410  - #               415              - - Trp Gly Leu His Asp Ile Ile Arg Ser Asn As - #p Trp Lys Ile Asn Gly                   420      - #           425      - #           430                   - - Ile Arg Glu Arg Ile Asp His Gln Glu Trp As - #n Pro Lys Val Asp Val               435          - #       440          - #       445                       - - His Leu Arg Ser Asp Gly Tyr Thr Asn Tyr Se - #r Leu Glu Thr Leu Asp           450              - #   455              - #   460                           - - Ala Gly Lys Arg Gln Cys Lys Ala Ala Leu Gl - #n Arg Asp Val Gly Leu       465                 4 - #70                 4 - #75                 4 -       #80                                                                               - - Glu Val Arg Asp Asp Val Pro Leu Leu Gly Ph - #e Ile Gly Arg Leu         Asp                                                                                              485  - #               490  - #               495              - - Gly Gln Lys Gly Val Asp Ile Ile Gly Asp Al - #a Met Pro Trp Ile Ala                   500      - #           505      - #           510                   - - Gly Gln Asp Val Gln Leu Val Met Leu Gly Th - #r Gly Pro Pro Asp Leu               515          - #       520          - #       525                       - - Glu Arg Met Leu Gln His Leu Glu Arg Glu Hi - #s Pro Asn Lys Val Arg           530              - #   535              - #   540                           - - Gly Trp Val Gly Phe Ser Val Leu Met Val Hi - #s Arg Ile Thr Pro Gly       545                 5 - #50                 5 - #55                 5 -       #60                                                                               - - Ala Ser Val Leu Val Met Pro Ser Arg Phe Al - #a Gly Gly Leu Asn         Gln                                                                                              565  - #               570  - #               575              - - Leu Tyr Ala Met Ala Tyr Gly Thr Val Pro Va - #l Val His Ala Val Gly                   580      - #           585      - #           590                   - - Gly Leu Arg Asp Thr Val Ala Pro Phe Asp Pr - #o Phe Gly Asp Ala Gly               595          - #       600          - #       605                       - - Leu Gly Trp Thr Phe Asp Arg Ala Glu Ala As - #n Lys Leu Ile Glu Val           610              - #   615              - #   620                           - - Leu Ser His Cys Leu Asp Thr Tyr Arg Asn Ty - #r Glu Glu Ser Trp Lys       625                 6 - #30                 6 - #35                 6 -       #40                                                                               - - Ser Leu Gln Ala Arg Gly Met Ser Gln Asn Le - #u Ser Trp Asp His         Ala                                                                                              645  - #               650  - #               655              - - Ala Glu Leu Tyr Glu Asp Val Leu Val Lys Ty - #r Gln Trp                               660      - #           665                                          - -  - - (2) INFORMATION FOR SEQ ID NO:10:                                     - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 2097 base - #pairs                                                 (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: double                                                       (D) TOPOLOGY: Not Relev - #ant                                        - -     (ii) MOLECULE TYPE: cDNA to mRNA                                       - -    (iii) HYPOTHETICAL: NO                                                  - -     (vi) ORIGINAL SOURCE:                                                           (A) ORGANISM: Zea mays                                                - -     (ix) FEATURE:                                                                   (A) NAME/KEY: CDS                                                              (B) LOCATION: 1..2097                                                 - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:10:                               - - ATG CCG GGG GCA ATC TCT TCC TCG TCG TCG GC - #T TTT CTC CTC CCC GTC            48                                                                        Met Pro Gly Ala Ile Ser Ser Ser Ser Ser Al - #a Phe Leu Leu Pro Val            670                 6 - #75                 6 - #80                 6 -       #85                                                                               - - GCG TCC TCC TCG CCG CGG CGC AGG CGG GGC AG - #T GTG GGT GCT GCT         CTG       96                                                                     Ala Ser Ser Ser Pro Arg Arg Arg Arg Gly Se - #r Val Gly Ala Ala Leu                           690  - #               695  - #               700               - - CGC TCG TAC GGC TAC AGC GGC GCG GAG CTG CG - #G TTG CAT TGG GCG CGG           144                                                                        Arg Ser Tyr Gly Tyr Ser Gly Ala Glu Leu Ar - #g Leu His Trp Ala Arg                        705      - #           710      - #           715                   - - CGG GGC CCG CCT CAG GAT GGA GCG GCG TCG GT - #A CGC GCC GCA GCG GCA           192                                                                        Arg Gly Pro Pro Gln Asp Gly Ala Ala Ser Va - #l Arg Ala Ala Ala Ala                    720          - #       725          - #       730                       - - CCG GCC GGG GGC GAA AGC GAG GAG GCA GCG AA - #G AGC TCC TCC TCG TCC           240                                                                        Pro Ala Gly Gly Glu Ser Glu Glu Ala Ala Ly - #s Ser Ser Ser Ser Ser                735              - #   740              - #   745                           - - CAG GCG GGC GCT GTT CAG GGC AGC ACG GCC AA - #G GCT GTG GAT TCT GCT           288                                                                        Gln Ala Gly Ala Val Gln Gly Ser Thr Ala Ly - #s Ala Val Asp Ser Ala            750                 7 - #55                 7 - #60                 7 -       #65                                                                               - - TCA CCT CCC AAT CCT TTG ACA TCT GCT CCG AA - #G CAA AGT CAG AGC         GCT      336                                                                     Ser Pro Pro Asn Pro Leu Thr Ser Ala Pro Ly - #s Gln Ser Gln Ser Ala                           770  - #               775  - #               780               - - GCA ATG CAA AAC GGA ACG AGT GGG GGC AGC AG - #C GCG AGC ACC GCC GCG           384                                                                        Ala Met Gln Asn Gly Thr Ser Gly Gly Ser Se - #r Ala Ser Thr Ala Ala                        785      - #           790      - #           795                   - - CCG GTG TCC GGA CCC AAA GCT GAT CAT CCA TC - #A GCT CCT GTC ACC AAG           432                                                                        Pro Val Ser Gly Pro Lys Ala Asp His Pro Se - #r Ala Pro Val Thr Lys                    800          - #       805          - #       810                       - - AGA GAA ATC GAT GCC AGT GCG GTG AAG CCA GA - #G CCC GCA GGT GAT GAT           480                                                                        Arg Glu Ile Asp Ala Ser Ala Val Lys Pro Gl - #u Pro Ala Gly Asp Asp                815              - #   820              - #   825                           - - GCT AGA CCG GTG GAA AGC ATA GGC ATC GCT GA - #A CCG GTG GAT GCT AAG           528                                                                        Ala Arg Pro Val Glu Ser Ile Gly Ile Ala Gl - #u Pro Val Asp Ala Lys            830                 8 - #35                 8 - #40                 8 -       #45                                                                               - - GCT GAT GCA GCT CCG GCT ACA GAT GCG GCG GC - #G AGT GCT CCT TAT         GAC      576                                                                     Ala Asp Ala Ala Pro Ala Thr Asp Ala Ala Al - #a Ser Ala Pro Tyr Asp                           850  - #               855  - #               860               - - AGG GAG GAT AAT GAA CCT GGC CCT TTG GCT GG - #G CCT AAT GTG ATG AAC           624                                                                        Arg Glu Asp Asn Glu Pro Gly Pro Leu Ala Gl - #y Pro Asn Val Met Asn                        865      - #           870      - #           875                   - - GTC GTC GTG GTG GCT TCT GAA TGT GCT CCT TT - #C TGC AAG ACA GGT GGC           672                                                                        Val Val Val Val Ala Ser Glu Cys Ala Pro Ph - #e Cys Lys Thr Gly Gly                    880          - #       885          - #       890                       - - CTT GGA GAT GTC GTG GGT GCT TTG CCT AAG GC - #T CTG GCG AGG AGA GGA           720                                                                        Leu Gly Asp Val Val Gly Ala Leu Pro Lys Al - #a Leu Ala Arg Arg Gly                895              - #   900              - #   905                           - - CAC CGT GTT ATG GTC GTG ATA CCA AGA TAT GG - #A GAG TAT GCC GAA GCC           768                                                                        His Arg Val Met Val Val Ile Pro Arg Tyr Gl - #y Glu Tyr Ala Glu Ala            910                 9 - #15                 9 - #20                 9 -       #25                                                                               - - CGG GAT TTA GGT GTA AGG AGA CGT TAC AAG GT - #A GCT GGA CAG GAT         TCA      816                                                                     Arg Asp Leu Gly Val Arg Arg Arg Tyr Lys Va - #l Ala Gly Gln Asp Ser                           930  - #               935  - #               940               - - GAA GTT ACT TAT TTT CAC TCT TAC ATT GAT GG - #A GTT GAT TTT GTA TTC           864                                                                        Glu Val Thr Tyr Phe His Ser Tyr Ile Asp Gl - #y Val Asp Phe Val Phe                        945      - #           950      - #           955                   - - GTA GAA GCC CCT CCC TTC CGG CAC CGG CAC AA - #T AAT ATT TAT GGG GGA           912                                                                        Val Glu Ala Pro Pro Phe Arg His Arg His As - #n Asn Ile Tyr Gly Gly                    960          - #       965          - #       970                       - - GAA AGA TTG GAT ATT TTG AAG CGC ATG ATT TT - #G TTC TGC AAG GCC GCT           960                                                                        Glu Arg Leu Asp Ile Leu Lys Arg Met Ile Le - #u Phe Cys Lys Ala Ala                975              - #   980              - #   985                           - - GTT GAG GTT CCA TGG TAT GCT CCA TGT GGC GG - #T ACT GTC TAT GGT GAT          1008                                                                        Val Glu Val Pro Trp Tyr Ala Pro Cys Gly Gl - #y Thr Val Tyr Gly Asp            990                 9 - #95                 1 - #000                1005        - - GGC AAC TTA GTT TTC ATT GCT AAT GAT TGG CA - #T ACC GCA CTT CTG CCT          1056                                                                        Gly Asn Leu Val Phe Ile Ala Asn Asp Trp Hi - #s Thr Ala Leu Leu Pro                            1010 - #               1015  - #              1020              - - GTC TAT CTA AAG GCC TAT TAC CGG GAC AAT GG - #T TTG ATG CAG TAT GCT          1104                                                                        Val Tyr Leu Lys Ala Tyr Tyr Arg Asp Asn Gl - #y Leu Met Gln Tyr Ala                        1025     - #           1030      - #          1035                  - - CGC TCT GTG CTT GTG ATA CAC AAC ATT GCT CA - #T CAG GGT CGT GGC CCT          1152                                                                        Arg Ser Val Leu Val Ile His Asn Ile Ala Hi - #s Gln Gly Arg Gly Pro                    1040         - #       1045          - #      1050                      - - GTA GAC GAC TTC GTC AAT TTT GAC TTG CCT GA - #A CAC TAC ATC GAC CAC          1200                                                                        Val Asp Asp Phe Val Asn Phe Asp Leu Pro Gl - #u His Tyr Ile Asp His                1055             - #   1060              - #  1065                          - - TTC AAA CTG TAT GAC AAC ATT GGT GGG GAT CA - #C AGC AAC GTT TTT GCT          1248                                                                        Phe Lys Leu Tyr Asp Asn Ile Gly Gly Asp Hi - #s Ser Asn Val Phe Ala            1070                1075 - #                1080 - #               1085         - - GCG GGG CTG AAG ACG GCA GAC CGG GTG GTG AC - #C GTT AGC AAT GGC TAC          1296                                                                        Ala Gly Leu Lys Thr Ala Asp Arg Val Val Th - #r Val Ser Asn Gly Tyr                            1090 - #               1095  - #              1100              - - ATG TGG GAG CTG AAG ACT TCG GAA GGC GGG TG - #G GGC CTC CAC GAC ATC          1344                                                                        Met Trp Glu Leu Lys Thr Ser Glu Gly Gly Tr - #p Gly Leu His Asp Ile                        1105     - #           1110      - #          1115                  - - ATA AAC CAG AAC GAC TGG AAG CTG CAG GGC AT - #C GTG AAC GGC ATC GAC          1392                                                                        Ile Asn Gln Asn Asp Trp Lys Leu Gln Gly Il - #e Val Asn Gly Ile Asp                    1120         - #       1125          - #      1130                      - - ATG AGC GAG TGG AAC CCC GCT GTG GAC GTG CA - #C CTC CAC TCC GAC GAC          1440                                                                        Met Ser Glu Trp Asn Pro Ala Val Asp Val Hi - #s Leu His Ser Asp Asp                1135             - #   1140              - #  1145                          - - TAC ACC AAC TAC ACG TTC GAG ACG CTG GAC AC - #C GGC AAG CGG CAG TGC          1488                                                                        Tyr Thr Asn Tyr Thr Phe Glu Thr Leu Asp Th - #r Gly Lys Arg Gln Cys            1150                1155 - #                1160 - #               1165         - - AAG GCC GCC CTG CAG CGG CAG CTG GGC CTG CA - #G GTC CGC GAC GAC GTG          1536                                                                        Lys Ala Ala Leu Gln Arg Gln Leu Gly Leu Gl - #n Val Arg Asp Asp Val                            1170 - #               1175  - #              1180              - - CCA CTG ATC GGG TTC ATC GGG CGG CTG GAC CA - #C CAG AAG GGC GTG GAC          1584                                                                        Pro Leu Ile Gly Phe Ile Gly Arg Leu Asp Hi - #s Gln Lys Gly Val Asp                        1185     - #           1190      - #          1195                  - - ATC ATC GCC GAC GCG ATC CAC TGG ATC GCG GG - #G CAG GAC GTG CAG CTC          1632                                                                        Ile Ile Ala Asp Ala Ile His Trp Ile Ala Gl - #y Gln Asp Val Gln Leu                    1200         - #       1205          - #      1210                      - - GTG ATG CTG GGC ACC GGG CGG GCC GAC CTG GA - #G GAC ATG CTG CGG CGG          1680                                                                        Val Met Leu Gly Thr Gly Arg Ala Asp Leu Gl - #u Asp Met Leu Arg Arg                1215             - #   1220              - #  1225                          - - TTC GAG TCG GAG CAC AGC GAC AAG GTG CGC GC - #G TGG GTG GGG TTC TCG          1728                                                                        Phe Glu Ser Glu His Ser Asp Lys Val Arg Al - #a Trp Val Gly Phe Ser            1230                1235 - #                1240 - #               1245         - - GTG CCC CTG GCG CAC CGC ATC ACG GCG GGC GC - #G GAC ATC CTG CTG ATG          1776                                                                        Val Pro Leu Ala His Arg Ile Thr Ala Gly Al - #a Asp Ile Leu Leu Met                            1250 - #               1255  - #              1260              - - CCG TCG CGG TTC GAG CCG TGC GGG CTG AAC CA - #G CTC TAC GCC ATG GCG          1824                                                                        Pro Ser Arg Phe Glu Pro Cys Gly Leu Asn Gl - #n Leu Tyr Ala Met Ala                        1265     - #           1270      - #          1275                  - - TAC GGG ACC GTG CCC GTG GTG CAC GCC GTG GG - #G GGG CTC CGG GAC ACG          1872                                                                        Tyr Gly Thr Val Pro Val Val His Ala Val Gl - #y Gly Leu Arg Asp Thr                    1280         - #       1285          - #      1290                      - - GTG GCG CCG TTC GAC CCG TTC AAC GAC ACC GG - #G CTC GGG TGG ACG TTC          1920                                                                        Val Ala Pro Phe Asp Pro Phe Asn Asp Thr Gl - #y Leu Gly Trp Thr Phe                1295             - #   1300              - #  1305                          - - GAC CGC GCG GAG GCG AAC CGG ATG ATC GAC GC - #G CTC TCG CAC TGC CTC          1968                                                                        Asp Arg Ala Glu Ala Asn Arg Met Ile Asp Al - #a Leu Ser His Cys Leu            1310                1315 - #                1320 - #               1325         - - ACC ACG TAC CGG AAC TAC AAG GAG AGC TGG CG - #C GCC TGC AGG GCG CGC          2016                                                                        Thr Thr Tyr Arg Asn Tyr Lys Glu Ser Trp Ar - #g Ala Cys Arg Ala Arg                            1330 - #               1335  - #              1340              - - GGC ATG GCC GAG GAC CTC AGC TGG GAC CAC GC - #C GCC GTG CTG TAT GAG          2064                                                                        Gly Met Ala Glu Asp Leu Ser Trp Asp His Al - #a Ala Val Leu Tyr Glu                        1345     - #           1350      - #          1355                  - - GAC GTG CTC GTC AAG GCG AAG TAC CAG TGG TG - #A                  -       #       2097                                                                     Asp Val Leu Val Lys Ala Lys Tyr Gln Trp  - #*                                          1360         - #       1365                                             - -  - - (2) INFORMATION FOR SEQ ID NO:11:                                     - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH:  698 ami - #no acids                                               (B) TYPE: amino acid                                                           (D) TOPOLOGY: linear                                                  - -     (ii) MOLECULE TYPE: protein                                            - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:11:                               - - Met Pro Gly Ala Ile Ser Ser Ser Ser Ser Al - #a Phe Leu Leu Pro         Val                                                                                1               5 - #                 10 - #                 15              - - Ala Ser Ser Ser Pro Arg Arg Arg Arg Gly Se - #r Val Gly Ala Ala Leu                    20     - #             25     - #             30                   - - Arg Ser Tyr Gly Tyr Ser Gly Ala Glu Leu Ar - #g Leu His Trp Ala Arg                35         - #         40         - #         45                       - - Arg Gly Pro Pro Gln Asp Gly Ala Ala Ser Va - #l Arg Ala Ala Ala Ala            50             - #     55             - #     60                           - - Pro Ala Gly Gly Glu Ser Glu Glu Ala Ala Ly - #s Ser Ser Ser Ser Ser        65                 - # 70                 - # 75                 - # 80        - - Gln Ala Gly Ala Val Gln Gly Ser Thr Ala Ly - #s Ala Val Asp Ser Ala                        85 - #                 90 - #                 95               - - Ser Pro Pro Asn Pro Leu Thr Ser Ala Pro Ly - #s Gln Ser Gln Ser Ala                   100      - #           105      - #           110                   - - Ala Met Gln Asn Gly Thr Ser Gly Gly Ser Se - #r Ala Ser Thr Ala Ala               115          - #       120          - #       125                       - - Pro Val Ser Gly Pro Lys Ala Asp His Pro Se - #r Ala Pro Val Thr Lys           130              - #   135              - #   140                           - - Arg Glu Ile Asp Ala Ser Ala Val Lys Pro Gl - #u Pro Ala Gly Asp Asp       145                 1 - #50                 1 - #55                 1 -       #60                                                                               - - Ala Arg Pro Val Glu Ser Ile Gly Ile Ala Gl - #u Pro Val Asp Ala         Lys                                                                                              165  - #               170  - #               175              - - Ala Asp Ala Ala Pro Ala Thr Asp Ala Ala Al - #a Ser Ala Pro Tyr Asp                   180      - #           185      - #           190                   - - Arg Glu Asp Asn Glu Pro Gly Pro Leu Ala Gl - #y Pro Asn Val Met Asn               195          - #       200          - #       205                       - - Val Val Val Val Ala Ser Glu Cys Ala Pro Ph - #e Cys Lys Thr Gly Gly           210              - #   215              - #   220                           - - Leu Gly Asp Val Val Gly Ala Leu Pro Lys Al - #a Leu Ala Arg Arg Gly       225                 2 - #30                 2 - #35                 2 -       #40                                                                               - - His Arg Val Met Val Val Ile Pro Arg Tyr Gl - #y Glu Tyr Ala Glu         Ala                                                                                              245  - #               250  - #               255              - - Arg Asp Leu Gly Val Arg Arg Arg Tyr Lys Va - #l Ala Gly Gln Asp Ser                   260      - #           265      - #           270                   - - Glu Val Thr Tyr Phe His Ser Tyr Ile Asp Gl - #y Val Asp Phe Val Phe               275          - #       280          - #       285                       - - Val Glu Ala Pro Pro Phe Arg His Arg His As - #n Asn Ile Tyr Gly Gly           290              - #   295              - #   300                           - - Glu Arg Leu Asp Ile Leu Lys Arg Met Ile Le - #u Phe Cys Lys Ala Ala       305                 3 - #10                 3 - #15                 3 -       #20                                                                               - - Val Glu Val Pro Trp Tyr Ala Pro Cys Gly Gl - #y Thr Val Tyr Gly         Asp                                                                                              325  - #               330  - #               335              - - Gly Asn Leu Val Phe Ile Ala Asn Asp Trp Hi - #s Thr Ala Leu Leu Pro                   340      - #           345      - #           350                   - - Val Tyr Leu Lys Ala Tyr Tyr Arg Asp Asn Gl - #y Leu Met Gln Tyr Ala               355          - #       360          - #       365                       - - Arg Ser Val Leu Val Ile His Asn Ile Ala Hi - #s Gln Gly Arg Gly Pro           370              - #   375              - #   380                           - - Val Asp Asp Phe Val Asn Phe Asp Leu Pro Gl - #u His Tyr Ile Asp His       385                 3 - #90                 3 - #95                 4 -       #00                                                                               - - Phe Lys Leu Tyr Asp Asn Ile Gly Gly Asp Hi - #s Ser Asn Val Phe         Ala                                                                                              405  - #               410  - #               415              - - Ala Gly Leu Lys Thr Ala Asp Arg Val Val Th - #r Val Ser Asn Gly Tyr                   420      - #           425      - #           430                   - - Met Trp Glu Leu Lys Thr Ser Glu Gly Gly Tr - #p Gly Leu His Asp Ile               435          - #       440          - #       445                       - - Ile Asn Gln Asn Asp Trp Lys Leu Gln Gly Il - #e Val Asn Gly Ile Asp           450              - #   455              - #   460                           - - Met Ser Glu Trp Asn Pro Ala Val Asp Val Hi - #s Leu His Ser Asp Asp       465                 4 - #70                 4 - #75                 4 -       #80                                                                               - - Tyr Thr Asn Tyr Thr Phe Glu Thr Leu Asp Th - #r Gly Lys Arg Gln         Cys                                                                                              485  - #               490  - #               495              - - Lys Ala Ala Leu Gln Arg Gln Leu Gly Leu Gl - #n Val Arg Asp Asp Val                   500      - #           505      - #           510                   - - Pro Leu Ile Gly Phe Ile Gly Arg Leu Asp Hi - #s Gln Lys Gly Val Asp               515          - #       520          - #       525                       - - Ile Ile Ala Asp Ala Ile His Trp Ile Ala Gl - #y Gln Asp Val Gln Leu           530              - #   535              - #   540                           - - Val Met Leu Gly Thr Gly Arg Ala Asp Leu Gl - #u Asp Met Leu Arg Arg       545                 5 - #50                 5 - #55                 5 -       #60                                                                               - - Phe Glu Ser Glu His Ser Asp Lys Val Arg Al - #a Trp Val Gly Phe         Ser                                                                                              565  - #               570  - #               575              - - Val Pro Leu Ala His Arg Ile Thr Ala Gly Al - #a Asp Ile Leu Leu Met                   580      - #           585      - #           590                   - - Pro Ser Arg Phe Glu Pro Cys Gly Leu Asn Gl - #n Leu Tyr Ala Met Ala               595          - #       600          - #       605                       - - Tyr Gly Thr Val Pro Val Val His Ala Val Gl - #y Gly Leu Arg Asp Thr           610              - #   615              - #   620                           - - Val Ala Pro Phe Asp Pro Phe Asn Asp Thr Gl - #y Leu Gly Trp Thr Phe       625                 6 - #30                 6 - #35                 6 -       #40                                                                               - - Asp Arg Ala Glu Ala Asn Arg Met Ile Asp Al - #a Leu Ser His Cys         Leu                                                                                              645  - #               650  - #               655              - - Thr Thr Tyr Arg Asn Tyr Lys Glu Ser Trp Ar - #g Ala Cys Arg Ala Arg                   660      - #           665      - #           670                   - - Gly Met Ala Glu Asp Leu Ser Trp Asp His Al - #a Ala Val Leu Tyr Glu               675          - #       680          - #       685                       - - Asp Val Leu Val Lys Ala Lys Tyr Gln Trp                                       690              - #   695                                                  - -  - - (2) INFORMATION FOR SEQ ID NO:12:                                     - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 1752 base - #pairs                                                 (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: double                                                       (D) TOPOLOGY: Not Relev - #ant                                        - -     (ii) MOLECULE TYPE: cDNA to mRNA                                       - -    (iii) HYPOTHETICAL: NO                                                  - -     (vi) ORIGINAL SOURCE:                                                           (A) ORGANISM: Zea mays                                                - -     (ix) FEATURE:                                                                   (A) NAME/KEY: CDS                                                              (B) LOCATION: 1..1752                                                 - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:12:                               - - TGC GTC GCG GAG CTG AGC AGG GAG GGG CCC GC - #G CCG CGC CCG CTG CCA            48                                                                        Cys Val Ala Glu Leu Ser Arg Glu Gly Pro Al - #a Pro Arg Pro Leu Pro            700                 7 - #05                 7 - #10                 7 -       #15                                                                               - - CCC GCG CTG CTG GCG CCC CCG CTC GTG CCC GG - #C TTC CTC GCG CCG         CCG       96                                                                     Pro Ala Leu Leu Ala Pro Pro Leu Val Pro Gl - #y Phe Leu Ala Pro Pro                           720  - #               725  - #               730               - - GCC GAG CCC ACG GGT GAG CCG GCA TCG ACG CC - #G CCG CCC GTG CCC GAC           144                                                                        Ala Glu Pro Thr Gly Glu Pro Ala Ser Thr Pr - #o Pro Pro Val Pro Asp                        735      - #           740      - #           745                   - - GCC GGC CTG GGG GAC CTC GGT CTC GAA CCT GA - #A GGG ATT GCT GAA GGT           192                                                                        Ala Gly Leu Gly Asp Leu Gly Leu Glu Pro Gl - #u Gly Ile Ala Glu Gly                    750          - #       755          - #       760                       - - TCC ATC GAT AAC ACA GTA GTT GTG GCA AGT GA - #G CAA GAT TCT GAG ATT           240                                                                        Ser Ile Asp Asn Thr Val Val Val Ala Ser Gl - #u Gln Asp Ser Glu Ile                765              - #   770              - #   775                           - - GTG GTT GGA AAG GAG CAA GCT CGA GCT AAA GT - #A ACA CAA AGC ATT GTC           288                                                                        Val Val Gly Lys Glu Gln Ala Arg Ala Lys Va - #l Thr Gln Ser Ile Val            780                 7 - #85                 7 - #90                 7 -       #95                                                                               - - TTT GTA ACC GGC GAA GCT TCT CCT TAT GCA AA - #G TCT GGG GGT CTA         GGA      336                                                                     Phe Val Thr Gly Glu Ala Ser Pro Tyr Ala Ly - #s Ser Gly Gly Leu Gly                           800  - #               805  - #               810               - - GAT GTT TGT GGT TCA TTG CCA GTT GCT CTT GC - #T GCT CGT GGT CAC CGT           384                                                                        Asp Val Cys Gly Ser Leu Pro Val Ala Leu Al - #a Ala Arg Gly His Arg                        815      - #           820      - #           825                   - - GTG ATG GTT GTA ATG CCC AGA TAT TTA AAT GG - #T ACC TCC GAT AAG AAT           432                                                                        Val Met Val Val Met Pro Arg Tyr Leu Asn Gl - #y Thr Ser Asp Lys Asn                    830          - #       835          - #       840                       - - TAT GCA AAT GCA TTT TAC ACA GAA AAA CAC AT - #T CGG ATT CCA TGC TTT           480                                                                        Tyr Ala Asn Ala Phe Tyr Thr Glu Lys His Il - #e Arg Ile Pro Cys Phe                845              - #   850              - #   855                           - - GGC GGT GAA CAT GAA GTT ACC TTC TTC CAT GA - #G TAT AGA GAT TCA GTT           528                                                                        Gly Gly Glu His Glu Val Thr Phe Phe His Gl - #u Tyr Arg Asp Ser Val            860                 8 - #65                 8 - #70                 8 -       #75                                                                               - - GAC TGG GTG TTT GTT GAT CAT CCC TCA TAT CA - #C AGA CCT GGA AAT         TTA      576                                                                     Asp Trp Val Phe Val Asp His Pro Ser Tyr Hi - #s Arg Pro Gly Asn Leu                           880  - #               885  - #               890               - - TAT GGA GAT AAG TTT GGT GCT TTT GGT GAT AA - #T CAG TTC AGA TAC ACA           624                                                                        Tyr Gly Asp Lys Phe Gly Ala Phe Gly Asp As - #n Gln Phe Arg Tyr Thr                        895      - #           900      - #           905                   - - CTC CTT TGC TAT GCT GCA TGT GAG GCT CCT TT - #G ATC CTT GAA TTG GGA           672                                                                        Leu Leu Cys Tyr Ala Ala Cys Glu Ala Pro Le - #u Ile Leu Glu Leu Gly                    910          - #       915          - #       920                       - - GGA TAT ATT TAT GGA CAG AAT TGC ATG TTT GT - #T GTC AAT GAT TGG CAT           720                                                                        Gly Tyr Ile Tyr Gly Gln Asn Cys Met Phe Va - #l Val Asn Asp Trp His                925              - #   930              - #   935                           - - GCC AGT CTA GTG CCA GTC CTT CTT GCT GCA AA - #A TAT AGA CCA TAT GGT           768                                                                        Ala Ser Leu Val Pro Val Leu Leu Ala Ala Ly - #s Tyr Arg Pro Tyr Gly            940                 9 - #45                 9 - #50                 9 -       #55                                                                               - - GTT TAT AAA GAC TCC CGC AGC ATT CTT GTA AT - #A CAT AAT TTA GCA         CAT      816                                                                     Val Tyr Lys Asp Ser Arg Ser Ile Leu Val Il - #e His Asn Leu Ala His                           960  - #               965  - #               970               - - CAG GGT GTA GAG CCT GCA AGC ACA TAT CCT GA - #C CTT GGG TTG CCA CCT           864                                                                        Gln Gly Val Glu Pro Ala Ser Thr Tyr Pro As - #p Leu Gly Leu Pro Pro                        975      - #           980      - #           985                   - - GAA TGG TAT GGA GCT CTG GAG TGG GTA TTC CC - #T GAA TGG GCG AGG AGG           912                                                                        Glu Trp Tyr Gly Ala Leu Glu Trp Val Phe Pr - #o Glu Trp Ala Arg Arg                    990          - #       995          - #       1000                      - - CAT GCC CTT GAC AAG GGT GAG GCA GTT AAT TT - #T TTG AAA GGT GCA GTT           960                                                                        His Ala Leu Asp Lys Gly Glu Ala Val Asn Ph - #e Leu Lys Gly Ala Val                1005             - #   1010              - #  1015                          - - GTG ACA GCA GAT CGA ATC GTG ACT GTC AGT AA - #G GGT TAT TCG TGG GAG          1008                                                                        Val Thr Ala Asp Arg Ile Val Thr Val Ser Ly - #s Gly Tyr Ser Trp Glu            1020                1025 - #                1030 - #               1035         - - GTC ACA ACT GCT GAA GGT GGA CAG GGC CTC AA - #T GAG CTC TTA AGC TCC          1056                                                                        Val Thr Thr Ala Glu Gly Gly Gln Gly Leu As - #n Glu Leu Leu Ser Ser                            1040 - #               1045  - #              1050              - - AGA AAG AGT GTA TTA AAC GGA ATT GTA AAT GG - #A ATT GAC ATT AAT GAT          1104                                                                        Arg Lys Ser Val Leu Asn Gly Ile Val Asn Gl - #y Ile Asp Ile Asn Asp                        1055     - #           1060      - #          1065                  - - TGG AAC CCT GCC ACA GAC AAA TGT ATC CCC TG - #T CAT TAT TCT GTT GAT          1152                                                                        Trp Asn Pro Ala Thr Asp Lys Cys Ile Pro Cy - #s His Tyr Ser Val Asp                    1070         - #       1075          - #      1080                      - - GAC CTC TCT GGA AAG GCC AAA TGT AAA GGT GC - #A TTG CAG AAG GAG CTG          1200                                                                        Asp Leu Ser Gly Lys Ala Lys Cys Lys Gly Al - #a Leu Gln Lys Glu Leu                1085             - #   1090              - #  1095                          - - GGT TTA CCT ATA AGG CCT GAT GTT CCT CTG AT - #T GGC TTT ATT GGA AGG          1248                                                                        Gly Leu Pro Ile Arg Pro Asp Val Pro Leu Il - #e Gly Phe Ile Gly Arg            1100                1105 - #                1110 - #               1115         - - TTG GAT TAT CAG AAA GGC ATT GAT CTC ATT CA - #A CTT ATC ATA CCA GAT          1296                                                                        Leu Asp Tyr Gln Lys Gly Ile Asp Leu Ile Gl - #n Leu Ile Ile Pro Asp                            1120 - #               1125  - #              1130              - - CTC ATG CGG GAA GAT GTT CAA TTT GTC ATG CT - #T GGA TCT GGT GAC CCA          1344                                                                        Leu Met Arg Glu Asp Val Gln Phe Val Met Le - #u Gly Ser Gly Asp Pro                        1135     - #           1140      - #          1145                  - - GAG CTT GAA GAT TGG ATG AGA TCT ACA GAG TC - #G ATC TTC AAG GAT AAA          1392                                                                        Glu Leu Glu Asp Trp Met Arg Ser Thr Glu Se - #r Ile Phe Lys Asp Lys                    1150         - #       1155          - #      1160                      - - TTT CGT GGA TGG GTT GGA TTT AGT GTT CCA GT - #T TCC CAC CGA ATA ACT          1440                                                                        Phe Arg Gly Trp Val Gly Phe Ser Val Pro Va - #l Ser His Arg Ile Thr                1165             - #   1170              - #  1175                          - - GCC GGC TGC GAT ATA TTG TTA ATG CCA TCC AG - #A TTC GAA CCT TGT GGT          1488                                                                        Ala Gly Cys Asp Ile Leu Leu Met Pro Ser Ar - #g Phe Glu Pro Cys Gly            1180                1185 - #                1190 - #               1195         - - CTC AAT CAG CTA TAT GCT ATG CAG TAT GGC AC - #A GTT CCT GTT GTC CAT          1536                                                                        Leu Asn Gln Leu Tyr Ala Met Gln Tyr Gly Th - #r Val Pro Val Val His                            1200 - #               1205  - #              1210              - - GCA ACT GGG GGC CTT AGA GAT ACC GTG GAG AA - #C TTC AAC CCT TTC GGT          1584                                                                        Ala Thr Gly Gly Leu Arg Asp Thr Val Glu As - #n Phe Asn Pro Phe Gly                        1215     - #           1220      - #          1225                  - - GAG AAT GGA GAG CAG GGT ACA GGG TGG GCA TT - #C GCA CCC CTA ACC ACA          1632                                                                        Glu Asn Gly Glu Gln Gly Thr Gly Trp Ala Ph - #e Ala Pro Leu Thr Thr                    1230         - #       1235          - #      1240                      - - GAA AAC ATG TTT GTG GAC ATT GCG AAC TGC AA - #T ATC TAC ATA CAG GGA          1680                                                                        Glu Asn Met Phe Val Asp Ile Ala Asn Cys As - #n Ile Tyr Ile Gln Gly                1245             - #   1250              - #  1255                          - - ACA CAA GTC CTC CTG GGA AGG GCT AAT GAA GC - #G AGG CAT GTC AAA AGA          1728                                                                        Thr Gln Val Leu Leu Gly Arg Ala Asn Glu Al - #a Arg His Val Lys Arg            1260                1265 - #                1270 - #               1275         - - CTT CAC GTG GGA CCA TGC CGC TGA     - #                  - #                   1752                                                                      Leu His Val Gly Pro Cys Arg  *                                                                 1280                                                            - -  - - (2) INFORMATION FOR SEQ ID NO:13:                                     - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH:  583 ami - #no acids                                               (B) TYPE: amino acid                                                           (D) TOPOLOGY: linear                                                  - -     (ii) MOLECULE TYPE: protein                                            - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:13:                               - - Cys Val Ala Glu Leu Ser Arg Glu Gly Pro Al - #a Pro Arg Pro Leu Pro         1               5 - #                 10 - #                 15               - - Pro Ala Leu Leu Ala Pro Pro Leu Val Pro Gl - #y Phe Leu Ala Pro Pro                    20     - #             25     - #             30                   - - Ala Glu Pro Thr Gly Glu Pro Ala Ser Thr Pr - #o Pro Pro Val Pro Asp                35         - #         40         - #         45                       - - Ala Gly Leu Gly Asp Leu Gly Leu Glu Pro Gl - #u Gly Ile Ala Glu Gly            50             - #     55             - #     60                           - - Ser Ile Asp Asn Thr Val Val Val Ala Ser Gl - #u Gln Asp Ser Glu Ile        65                 - # 70                 - # 75                 - # 80        - - Val Val Gly Lys Glu Gln Ala Arg Ala Lys Va - #l Thr Gln Ser Ile Val                        85 - #                 90 - #                 95               - - Phe Val Thr Gly Glu Ala Ser Pro Tyr Ala Ly - #s Ser Gly Gly Leu Gly                   100      - #           105      - #           110                   - - Asp Val Cys Gly Ser Leu Pro Val Ala Leu Al - #a Ala Arg Gly His Arg               115          - #       120          - #       125                       - - Val Met Val Val Met Pro Arg Tyr Leu Asn Gl - #y Thr Ser Asp Lys Asn           130              - #   135              - #   140                           - - Tyr Ala Asn Ala Phe Tyr Thr Glu Lys His Il - #e Arg Ile Pro Cys Phe       145                 1 - #50                 1 - #55                 1 -       #60                                                                               - - Gly Gly Glu His Glu Val Thr Phe Phe His Gl - #u Tyr Arg Asp Ser         Val                                                                                              165  - #               170  - #               175              - - Asp Trp Val Phe Val Asp His Pro Ser Tyr Hi - #s Arg Pro Gly Asn Leu                   180      - #           185      - #           190                   - - Tyr Gly Asp Lys Phe Gly Ala Phe Gly Asp As - #n Gln Phe Arg Tyr Thr               195          - #       200          - #       205                       - - Leu Leu Cys Tyr Ala Ala Cys Glu Ala Pro Le - #u Ile Leu Glu Leu Gly           210              - #   215              - #   220                           - - Gly Tyr Ile Tyr Gly Gln Asn Cys Met Phe Va - #l Val Asn Asp Trp His       225                 2 - #30                 2 - #35                 2 -       #40                                                                               - - Ala Ser Leu Val Pro Val Leu Leu Ala Ala Ly - #s Tyr Arg Pro Tyr         Gly                                                                                              245  - #               250  - #               255              - - Val Tyr Lys Asp Ser Arg Ser Ile Leu Val Il - #e His Asn Leu Ala His                   260      - #           265      - #           270                   - - Gln Gly Val Glu Pro Ala Ser Thr Tyr Pro As - #p Leu Gly Leu Pro Pro               275          - #       280          - #       285                       - - Glu Trp Tyr Gly Ala Leu Glu Trp Val Phe Pr - #o Glu Trp Ala Arg Arg           290              - #   295              - #   300                           - - His Ala Leu Asp Lys Gly Glu Ala Val Asn Ph - #e Leu Lys Gly Ala Val       305                 3 - #10                 3 - #15                 3 -       #20                                                                               - - Val Thr Ala Asp Arg Ile Val Thr Val Ser Ly - #s Gly Tyr Ser Trp         Glu                                                                                              325  - #               330  - #               335              - - Val Thr Thr Ala Glu Gly Gly Gln Gly Leu As - #n Glu Leu Leu Ser Ser                   340      - #           345      - #           350                   - - Arg Lys Ser Val Leu Asn Gly Ile Val Asn Gl - #y Ile Asp Ile Asn Asp               355          - #       360          - #       365                       - - Trp Asn Pro Ala Thr Asp Lys Cys Ile Pro Cy - #s His Tyr Ser Val Asp           370              - #   375              - #   380                           - - Asp Leu Ser Gly Lys Ala Lys Cys Lys Gly Al - #a Leu Gln Lys Glu Leu       385                 3 - #90                 3 - #95                 4 -       #00                                                                               - - Gly Leu Pro Ile Arg Pro Asp Val Pro Leu Il - #e Gly Phe Ile Gly         Arg                                                                                              405  - #               410  - #               415              - - Leu Asp Tyr Gln Lys Gly Ile Asp Leu Ile Gl - #n Leu Ile Ile Pro Asp                   420      - #           425      - #           430                   - - Leu Met Arg Glu Asp Val Gln Phe Val Met Le - #u Gly Ser Gly Asp Pro               435          - #       440          - #       445                       - - Glu Leu Glu Asp Trp Met Arg Ser Thr Glu Se - #r Ile Phe Lys Asp Lys           450              - #   455              - #   460                           - - Phe Arg Gly Trp Val Gly Phe Ser Val Pro Va - #l Ser His Arg Ile Thr       465                 4 - #70                 4 - #75                 4 -       #80                                                                               - - Ala Gly Cys Asp Ile Leu Leu Met Pro Ser Ar - #g Phe Glu Pro Cys         Gly                                                                                              485  - #               490  - #               495              - - Leu Asn Gln Leu Tyr Ala Met Gln Tyr Gly Th - #r Val Pro Val Val His                   500      - #           505      - #           510                   - - Ala Thr Gly Gly Leu Arg Asp Thr Val Glu As - #n Phe Asn Pro Phe Gly               515          - #       520          - #       525                       - - Glu Asn Gly Glu Gln Gly Thr Gly Trp Ala Ph - #e Ala Pro Leu Thr Thr           530              - #   535              - #   540                           - - Glu Asn Met Phe Val Asp Ile Ala Asn Cys As - #n Ile Tyr Ile Gln Gly       545                 5 - #50                 5 - #55                 5 -       #60                                                                               - - Thr Gln Val Leu Leu Gly Arg Ala Asn Glu Al - #a Arg His Val Lys         Arg                                                                                              565  - #               570  - #               575              - - Leu His Val Gly Pro Cys Arg                                                           580                                                                 - -  - - (2) INFORMATION FOR SEQ ID NO:14:                                     - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 2725 base - #pairs                                                 (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: Not Relev - #ant                                        - -     (ii) MOLECULE TYPE: mRNA                                               - -    (iii) HYPOTHETICAL: NO                                                  - -     (vi) ORIGINAL SOURCE:                                                           (A) ORGANISM: Zea mays                                                - -     (ix) FEATURE:                                                                   (A) NAME/KEY: sig.sub.-- - #peptide                                            (B) LOCATION: 91..264                                                 - -     (ix) FEATURE:                                                                   (A) NAME/KEY: mat.sub.-- - #peptide                                            (B) LOCATION: 265..2487                                               - -     (ix) FEATURE:                                                                   (A) NAME/KEY: CDS                                                              (B) LOCATION: 91..2490                                                - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:14:                               - - GGCCCAGAGC AGACCCGGAT TTCGCTCTTG CGGTCGCTGG GGTTTTAGCA TT -              #GGCTGATC     60                                                                  - - AGTTCGATCC GATCCGGCTG CGAAGGCGAG ATG GCG TTC CGG GT - #T TCT GGG        GCG     114                                                                                        - #               Met Ala - #Phe Arg Val Ser Gly Ala                          - #               -58  - #       -55                          - - GTG CTC GGT GGG GCC GTA AGG GCT CCC CGA CT - #C ACC GGC GGC GGG GAG           162                                                                        Val Leu Gly Gly Ala Val Arg Ala Pro Arg Le - #u Thr Gly Gly Gly Glu           50                 - - #45                 - - #40                 - -         #35                                                                               - - GGT AGT CTA GTC TTC CGG CAC ACC GGC CTC TT - #C TTA ACT CGG GGT         GCT      210                                                                     Gly Ser Leu Val Phe Arg His Thr Gly Leu Ph - #e Leu Thr Arg Gly Ala                           -30  - #               -25  - #               -20               - - CGA GTT GGA TGT TCG GGG ACG CAC GGG GCC AT - #G CGC GCG GCG GCC GCG           258                                                                        Arg Val Gly Cys Ser Gly Thr His Gly Ala Me - #t Arg Ala Ala Ala Ala                        -15      - #           -10      - #            -5                   - - GCC AGG AAG GCG GTC ATG GTT CCT GAG GGC GA - #G AAT GAT GGC CTC GCA           306                                                                        Ala Arg Lys Ala Val Met Val Pro Glu Gly Gl - #u Asn Asp Gly Leu Ala                      1        - #       5           - #       10                           - - TCA AGG GCT GAC TCG GCT CAA TTC CAG TCG GA - #T GAA CTG GAG GTA CCA           354                                                                        Ser Arg Ala Asp Ser Ala Gln Phe Gln Ser As - #p Glu Leu Glu Val Pro             15                 - # 20                 - # 25                 - # 30        - - GAC ATT TCT GAA GAG ACA ACG TGC GGT GCT GG - #T GTG GCT GAT GCT CAA           402                                                                        Asp Ile Ser Glu Glu Thr Thr Cys Gly Ala Gl - #y Val Ala Asp Ala Gln                             35 - #                 40 - #                 45               - - GCC TTG AAC AGA GTT CGA GTG GTC CCC CCA CC - #A AGC GAT GGA CAA AAA           450                                                                        Ala Leu Asn Arg Val Arg Val Val Pro Pro Pr - #o Ser Asp Gly Gln Lys                         50     - #             55     - #             60                   - - ATA TTC CAG ATT GAC CCC ATG TTG CAA GGC TA - #T AAG TAC CAT CTT GAG           498                                                                        Ile Phe Gln Ile Asp Pro Met Leu Gln Gly Ty - #r Lys Tyr His Leu Glu                     65         - #         70         - #         75                       - - TAT CGG TAC AGC CTC TAT AGA AGA ATC CGT TC - #A GAC ATT GAT GAA CAT           546                                                                        Tyr Arg Tyr Ser Leu Tyr Arg Arg Ile Arg Se - #r Asp Ile Asp Glu His                 80             - #     85             - #     90                           - - GAA GGA GGC TTG GAA GCC TTC TCC CGT AGT TA - #T GAG AAG TTT GGA TTT           594                                                                        Glu Gly Gly Leu Glu Ala Phe Ser Arg Ser Ty - #r Glu Lys Phe Gly Phe             95                 - #100                 - #105                 - #110        - - AAT GCC AGC GCG GAA GGT ATC ACA TAT CGA GA - #A TGG GCT CCT GGA GCA           642                                                                        Asn Ala Ser Ala Glu Gly Ile Thr Tyr Arg Gl - #u Trp Ala Pro Gly Ala                            115  - #               120  - #               125               - - TTT TCT GCA GCA TTG GTG GGT GAC GTC AAC AA - #C TGG GAT CCA AAT GCA           690                                                                        Phe Ser Ala Ala Leu Val Gly Asp Val Asn As - #n Trp Asp Pro Asn Ala                        130      - #           135      - #           140                   - - GAT CGT ATG AGC AAA AAT GAG TTT GGT GTT TG - #G GAA ATT TTT CTG CCT           738                                                                        Asp Arg Met Ser Lys Asn Glu Phe Gly Val Tr - #p Glu Ile Phe Leu Pro                    145          - #       150          - #       155                       - - AAC AAT GCA GAT GGT ACA TCA CCT ATT CCT CA - #T GGA TCT CGT GTA AAG           786                                                                        Asn Asn Ala Asp Gly Thr Ser Pro Ile Pro Hi - #s Gly Ser Arg Val Lys                160              - #   165              - #   170                           - - GTG AGA ATG GAT ACT CCA TCA GGG ATA AAG GA - #T TCA ATT CCA GCC TGG           834                                                                        Val Arg Met Asp Thr Pro Ser Gly Ile Lys As - #p Ser Ile Pro Ala Trp            175                 1 - #80                 1 - #85                 1 -       #90                                                                               - - ATC AAG TAC TCA GTG CAG GCC CCA GGA GAA AT - #A CCA TAT GAT GGG         ATT      882                                                                     Ile Lys Tyr Ser Val Gln Ala Pro Gly Glu Il - #e Pro Tyr Asp Gly Ile                           195  - #               200  - #               205               - - TAT TAT GAT CCT CCT GAA GAG GTA AAG TAT GT - #G TTC AGG CAT GCG CAA           930                                                                        Tyr Tyr Asp Pro Pro Glu Glu Val Lys Tyr Va - #l Phe Arg His Ala Gln                        210      - #           215      - #           220                   - - CCT AAA CGA CCA AAA TCA TTG CGG ATA TAT GA - #A ACA CAT GTC GGA ATG           978                                                                        Pro Lys Arg Pro Lys Ser Leu Arg Ile Tyr Gl - #u Thr His Val Gly Met                    225          - #       230          - #       235                       - - AGT AGC CCG GAA CCG AAG ATA AAC ACA TAT GT - #A AAC TTT AGG GAT GAA          1026                                                                        Ser Ser Pro Glu Pro Lys Ile Asn Thr Tyr Va - #l Asn Phe Arg Asp Glu                240              - #   245              - #   250                           - - GTC CTC CCA AGA ATA AAA AAA CTT GGA TAC AA - #T GCA GTG CAA ATA ATG          1074                                                                        Val Leu Pro Arg Ile Lys Lys Leu Gly Tyr As - #n Ala Val Gln Ile Met            255                 2 - #60                 2 - #65                 2 -       #70                                                                               - - GCA ATC CAA GAG CAC TCA TAT TAT GGA AGC TT - #T GGA TAC CAT GTA         ACT     1122                                                                     Ala Ile Gln Glu His Ser Tyr Tyr Gly Ser Ph - #e Gly Tyr His Val Thr                           275  - #               280  - #               285               - - AAT TTT TTT GCG CCA AGT AGT CGT TTT GGT AC - #C CCA GAA GAT TTG AAG          1170                                                                        Asn Phe Phe Ala Pro Ser Ser Arg Phe Gly Th - #r Pro Glu Asp Leu Lys                        290      - #           295      - #           300                   - - TCT TTG ATT GAT AGA GCA CAT GAG CTT GGT TT - #G CTA GTT CTC ATG GAT          1218                                                                        Ser Leu Ile Asp Arg Ala His Glu Leu Gly Le - #u Leu Val Leu Met Asp                    305          - #       310          - #       315                       - - GTG GTT CAT AGT CAT GCG TCA AGT AAT ACT CT - #G GAT GGG TTG AAT GGT          1266                                                                        Val Val His Ser His Ala Ser Ser Asn Thr Le - #u Asp Gly Leu Asn Gly                320              - #   325              - #   330                           - - TTT GAT GGT ACA GAT ACA CAT TAC TTT CAC AG - #T GGT CCA CGT GGC CAT          1314                                                                        Phe Asp Gly Thr Asp Thr His Tyr Phe His Se - #r Gly Pro Arg Gly His            335                 3 - #40                 3 - #45                 3 -       #50                                                                               - - CAC TGG ATG TGG GAT TCT CGC CTA TTT AAC TA - #T GGG AAC TGG GAA         GTT     1362                                                                     His Trp Met Trp Asp Ser Arg Leu Phe Asn Ty - #r Gly Asn Trp Glu Val                           355  - #               360  - #               365               - - TTA AGA TTT CTT CTC TCC AAT GCT AGA TGG TG - #G CTC GAG GAA TAT AAG          1410                                                                        Leu Arg Phe Leu Leu Ser Asn Ala Arg Trp Tr - #p Leu Glu Glu Tyr Lys                        370      - #           375      - #           380                   - - TTT GAT GGT TTC CGT TTT GAT GGT GTG ACC TC - #C ATG ATG TAC ACT CAC          1458                                                                        Phe Asp Gly Phe Arg Phe Asp Gly Val Thr Se - #r Met Met Tyr Thr His                    385          - #       390          - #       395                       - - CAC GGA TTA CAA GTA ACA TTT ACG GGG AAC TT - #C AAT GAG TAT TTT GGC          1506                                                                        His Gly Leu Gln Val Thr Phe Thr Gly Asn Ph - #e Asn Glu Tyr Phe Gly                400              - #   405              - #   410                           - - TTT GCC ACC GAT GTA GAT GCA GTG GTT TAC TT - #G ATG CTG GTA AAT GAT          1554                                                                        Phe Ala Thr Asp Val Asp Ala Val Val Tyr Le - #u Met Leu Val Asn Asp            415                 4 - #20                 4 - #25                 4 -       #30                                                                               - - CTA ATT CAT GGA CTT TAT CCT GAG GCT GTA AC - #C ATT GGT GAA GAT         GTT     1602                                                                     Leu Ile His Gly Leu Tyr Pro Glu Ala Val Th - #r Ile Gly Glu Asp Val                           435  - #               440  - #               445               - - AGT GGA ATG CCT ACA TTT GCC CTT CCT GTT CA - #C GAT GGT GGG GTA GGT          1650                                                                        Ser Gly Met Pro Thr Phe Ala Leu Pro Val Hi - #s Asp Gly Gly Val Gly                        450      - #           455      - #           460                   - - TTT GAC TAT CGG ATG CAT ATG GCT GTG GCT GA - #C AAA TGG ATT GAC CTT          1698                                                                        Phe Asp Tyr Arg Met His Met Ala Val Ala As - #p Lys Trp Ile Asp Leu                    465          - #       470          - #       475                       - - CTC AAG CAA AGT GAT GAA ACT TGG AAG ATG GG - #T GAT ATT GTG CAC ACA          1746                                                                        Leu Lys Gln Ser Asp Glu Thr Trp Lys Met Gl - #y Asp Ile Val His Thr                480              - #   485              - #   490                           - - CTG ACA AAT AGG AGG TGG TTA GAG AAG TGT GT - #A ACT TAT GCT GAA AGT          1794                                                                        Leu Thr Asn Arg Arg Trp Leu Glu Lys Cys Va - #l Thr Tyr Ala Glu Ser            495                 5 - #00                 5 - #05                 5 -       #10                                                                               - - CAT GAT CAA GCA TTA GTC GGC GAC AAG ACT AT - #T GCG TTT TGG TTG         ATG     1842                                                                     His Asp Gln Ala Leu Val Gly Asp Lys Thr Il - #e Ala Phe Trp Leu Met                           515  - #               520  - #               525               - - GAC AAG GAT ATG TAT GAT TTC ATG GCC CTC GA - #T AGA CCT TCA ACT CCT          1890                                                                        Asp Lys Asp Met Tyr Asp Phe Met Ala Leu As - #p Arg Pro Ser Thr Pro                        530      - #           535      - #           540                   - - ACC ATT GAT CGT GGG ATA GCA TTA CAT AAG AT - #G ATT AGA CTT ATC ACA          1938                                                                        Thr Ile Asp Arg Gly Ile Ala Leu His Lys Me - #t Ile Arg Leu Ile Thr                    545          - #       550          - #       555                       - - ATG GGT TTA GGA GGA GAG GGC TAT CTT AAT TT - #C ATG GGA AAT GAG TTT          1986                                                                        Met Gly Leu Gly Gly Glu Gly Tyr Leu Asn Ph - #e Met Gly Asn Glu Phe                560              - #   565              - #   570                           - - GGA CAT CCT GAA TGG ATA GAT TTT CCA AGA GG - #T CCG CAA AGA CTT CCA          2034                                                                        Gly His Pro Glu Trp Ile Asp Phe Pro Arg Gl - #y Pro Gln Arg Leu Pro            575                 5 - #80                 5 - #85                 5 -       #90                                                                               - - AGT GGT AAG TTT ATT CCA GGG AAT AAC AAC AG - #T TAT GAC AAA TGT         CGT     2082                                                                     Ser Gly Lys Phe Ile Pro Gly Asn Asn Asn Se - #r Tyr Asp Lys Cys Arg                           595  - #               600  - #               605               - - CGA AGA TTT GAC CTG GGT GAT GCA GAC TAT CT - #T AGG TAT CAT GGT ATG          2130                                                                        Arg Arg Phe Asp Leu Gly Asp Ala Asp Tyr Le - #u Arg Tyr His Gly Met                        610      - #           615      - #           620                   - - CAA GAG TTT GAT CAG GCA ATG CAA CAT CTT GA - #G CAA AAA TAT GAA TTC          2178                                                                        Gln Glu Phe Asp Gln Ala Met Gln His Leu Gl - #u Gln Lys Tyr Glu Phe                    625          - #       630          - #       635                       - - ATG ACA TCT GAT CAC CAG TAT ATT TCC CGG AA - #A CAT GAG GAG GAT AAG          2226                                                                        Met Thr Ser Asp His Gln Tyr Ile Ser Arg Ly - #s His Glu Glu Asp Lys                640              - #   645              - #   650                           - - GTG ATT GTG TTC GAA AAG GGA GAT TTG GTA TT - #T GTG TTC AAC TTC CAC          2274                                                                        Val Ile Val Phe Glu Lys Gly Asp Leu Val Ph - #e Val Phe Asn Phe His            655                 6 - #60                 6 - #65                 6 -       #70                                                                               - - TGC AAC AAC AGC TAT TTT GAC TAC CGT ATT GG - #T TGT CGA AAG CCT         GGG     2322                                                                     Cys Asn Asn Ser Tyr Phe Asp Tyr Arg Ile Gl - #y Cys Arg Lys Pro Gly                           675  - #               680  - #               685               - - GTG TAT AAG GTG GTC TTG GAC TCC GAC GCT GG - #A CTA TTT GGT GGA TTT          2370                                                                        Val Tyr Lys Val Val Leu Asp Ser Asp Ala Gl - #y Leu Phe Gly Gly Phe                        690      - #           695      - #           700                   - - AGC AGG ATC CAT CAC GCA GCC GAG CAC TTC AC - #C GCC GAC TGT TCG CAT          2418                                                                        Ser Arg Ile His His Ala Ala Glu His Phe Th - #r Ala Asp Cys Ser His                    705          - #       710          - #       715                       - - GAT AAT AGG CCA TAT TCA TTC TCG GTT TAT AC - #A CCA AGC AGA ACA TGT          2466                                                                        Asp Asn Arg Pro Tyr Ser Phe Ser Val Tyr Th - #r Pro Ser Arg Thr Cys                720              - #   725              - #   730                           - - GTC GTC TAT GCT CCA GTG GAG TGA TAGCGGGGTA CT - #CGTTGCTG CGCGGCATGT         2520                                                                        Val Val Tyr Ala Pro Val Glu  *                                                 735                 7 - #40                                                     - - GTGGGGCTGT CGATGTGAGG AAAAACCTTC TTCCAAAACC GGCAGATGCA TG -              #CATGCATG   2580                                                                  - - CTACAATAAG GTTCTGATAC TTTAATCGAT GCTGGAAAGC CCATGCATCT CG -             #CTGCGTTG   2640                                                                  - - TCCTCTCTAT ATATATAAGA CCTTCAAGGT GTCAATTAAA CATAGAGTTT TC -             #GTTTTTCG   2700                                                                  - - CTTTCCTAAA AAAAAAAAAA AAAAA          - #                  - #                  2725                                                                      - -  - - (2) INFORMATION FOR SEQ ID NO:15:                                     - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH:  799 ami - #no acids                                               (B) TYPE: amino acid                                                           (D) TOPOLOGY: linear                                                  - -     (ii) MOLECULE TYPE: protein                                            - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:15:                               - - Met Ala Phe Arg Val Ser Gly Ala Val Leu Gl - #y Gly Ala Val Arg Ala      58         -55        - #         -50        - #         -45                     - - Pro Arg Leu Thr Gly Gly Gly Glu Gly Ser Le - #u Val Phe Arg His Thr               -40          - #       -35          - #       -30                       - - Gly Leu Phe Leu Thr Arg Gly Ala Arg Val Gl - #y Cys Ser Gly Thr His           -25              - #   -20              - #   -15                           - - Gly Ala Met Arg Ala Ala Ala Ala Ala Arg Ly - #s Ala Val Met Val Pro      10                  - #-5                  - # 1               5                 - - Glu Gly Glu Asn Asp Gly Leu Ala Ser Arg Al - #a Asp Ser Ala Gln Phe                    10     - #             15     - #             20                   - - Gln Ser Asp Glu Leu Glu Val Pro Asp Ile Se - #r Glu Glu Thr Thr Cys                25         - #         30         - #         35                       - - Gly Ala Gly Val Ala Asp Ala Gln Ala Leu As - #n Arg Val Arg Val Val            40             - #     45             - #     50                           - - Pro Pro Pro Ser Asp Gly Gln Lys Ile Phe Gl - #n Ile Asp Pro Met Leu        55                 - # 60                 - # 65                 - # 70        - - Gln Gly Tyr Lys Tyr His Leu Glu Tyr Arg Ty - #r Ser Leu Tyr Arg Arg                        75 - #                 80 - #                 85               - - Ile Arg Ser Asp Ile Asp Glu His Glu Gly Gl - #y Leu Glu Ala Phe Ser                    90     - #             95     - #            100                   - - Arg Ser Tyr Glu Lys Phe Gly Phe Asn Ala Se - #r Ala Glu Gly Ile Thr               105          - #       110          - #       115                       - - Tyr Arg Glu Trp Ala Pro Gly Ala Phe Ser Al - #a Ala Leu Val Gly Asp           120              - #   125              - #   130                           - - Val Asn Asn Trp Asp Pro Asn Ala Asp Arg Me - #t Ser Lys Asn Glu Phe       135                 1 - #40                 1 - #45                 1 -       #50                                                                               - - Gly Val Trp Glu Ile Phe Leu Pro Asn Asn Al - #a Asp Gly Thr Ser         Pro                                                                                              155  - #               160  - #               165              - - Ile Pro His Gly Ser Arg Val Lys Val Arg Me - #t Asp Thr Pro Ser Gly                   170      - #           175      - #           180                   - - Ile Lys Asp Ser Ile Pro Ala Trp Ile Lys Ty - #r Ser Val Gln Ala Pro               185          - #       190          - #       195                       - - Gly Glu Ile Pro Tyr Asp Gly Ile Tyr Tyr As - #p Pro Pro Glu Glu Val           200              - #   205              - #   210                           - - Lys Tyr Val Phe Arg His Ala Gln Pro Lys Ar - #g Pro Lys Ser Leu Arg       215                 2 - #20                 2 - #25                 2 -       #30                                                                               - - Ile Tyr Glu Thr His Val Gly Met Ser Ser Pr - #o Glu Pro Lys Ile         Asn                                                                                              235  - #               240  - #               245              - - Thr Tyr Val Asn Phe Arg Asp Glu Val Leu Pr - #o Arg Ile Lys Lys Leu                   250      - #           255      - #           260                   - - Gly Tyr Asn Ala Val Gln Ile Met Ala Ile Gl - #n Glu His Ser Tyr Tyr               265          - #       270          - #       275                       - - Gly Ser Phe Gly Tyr His Val Thr Asn Phe Ph - #e Ala Pro Ser Ser Arg           280              - #   285              - #   290                           - - Phe Gly Thr Pro Glu Asp Leu Lys Ser Leu Il - #e Asp Arg Ala His Glu       295                 3 - #00                 3 - #05                 3 -       #10                                                                               - - Leu Gly Leu Leu Val Leu Met Asp Val Val Hi - #s Ser His Ala Ser         Ser                                                                                              315  - #               320  - #               325              - - Asn Thr Leu Asp Gly Leu Asn Gly Phe Asp Gl - #y Thr Asp Thr His Tyr                   330      - #           335      - #           340                   - - Phe His Ser Gly Pro Arg Gly His His Trp Me - #t Trp Asp Ser Arg Leu               345          - #       350          - #       355                       - - Phe Asn Tyr Gly Asn Trp Glu Val Leu Arg Ph - #e Leu Leu Ser Asn Ala           360              - #   365              - #   370                           - - Arg Trp Trp Leu Glu Glu Tyr Lys Phe Asp Gl - #y Phe Arg Phe Asp Gly       375                 3 - #80                 3 - #85                 3 -       #90                                                                               - - Val Thr Ser Met Met Tyr Thr His His Gly Le - #u Gln Val Thr Phe         Thr                                                                                              395  - #               400  - #               405              - - Gly Asn Phe Asn Glu Tyr Phe Gly Phe Ala Th - #r Asp Val Asp Ala Val                   410      - #           415      - #           420                   - - Val Tyr Leu Met Leu Val Asn Asp Leu Ile Hi - #s Gly Leu Tyr Pro Glu               425          - #       430          - #       435                       - - Ala Val Thr Ile Gly Glu Asp Val Ser Gly Me - #t Pro Thr Phe Ala Leu           440              - #   445              - #   450                           - - Pro Val His Asp Gly Gly Val Gly Phe Asp Ty - #r Arg Met His Met Ala       455                 4 - #60                 4 - #65                 4 -       #70                                                                               - - Val Ala Asp Lys Trp Ile Asp Leu Leu Lys Gl - #n Ser Asp Glu Thr         Trp                                                                                              475  - #               480  - #               485              - - Lys Met Gly Asp Ile Val His Thr Leu Thr As - #n Arg Arg Trp Leu Glu                   490      - #           495      - #           500                   - - Lys Cys Val Thr Tyr Ala Glu Ser His Asp Gl - #n Ala Leu Val Gly Asp               505          - #       510          - #       515                       - - Lys Thr Ile Ala Phe Trp Leu Met Asp Lys As - #p Met Tyr Asp Phe Met           520              - #   525              - #   530                           - - Ala Leu Asp Arg Pro Ser Thr Pro Thr Ile As - #p Arg Gly Ile Ala Leu       535                 5 - #40                 5 - #45                 5 -       #50                                                                               - - His Lys Met Ile Arg Leu Ile Thr Met Gly Le - #u Gly Gly Glu Gly         Tyr                                                                                              555  - #               560  - #               565              - - Leu Asn Phe Met Gly Asn Glu Phe Gly His Pr - #o Glu Trp Ile Asp Phe                   570      - #           575      - #           580                   - - Pro Arg Gly Pro Gln Arg Leu Pro Ser Gly Ly - #s Phe Ile Pro Gly Asn               585          - #       590          - #       595                       - - Asn Asn Ser Tyr Asp Lys Cys Arg Arg Arg Ph - #e Asp Leu Gly Asp Ala           600              - #   605              - #   610                           - - Asp Tyr Leu Arg Tyr His Gly Met Gln Glu Ph - #e Asp Gln Ala Met Gln       615                 6 - #20                 6 - #25                 6 -       #30                                                                               - - His Leu Glu Gln Lys Tyr Glu Phe Met Thr Se - #r Asp His Gln Tyr         Ile                                                                                              635  - #               640  - #               645              - - Ser Arg Lys His Glu Glu Asp Lys Val Ile Va - #l Phe Glu Lys Gly Asp                   650      - #           655      - #           660                   - - Leu Val Phe Val Phe Asn Phe His Cys Asn As - #n Ser Tyr Phe Asp Tyr               665          - #       670          - #       675                       - - Arg Ile Gly Cys Arg Lys Pro Gly Val Tyr Ly - #s Val Val Leu Asp Ser           680              - #   685              - #   690                           - - Asp Ala Gly Leu Phe Gly Gly Phe Ser Arg Il - #e His His Ala Ala Glu       695                 7 - #00                 7 - #05                 7 -       #10                                                                               - - His Phe Thr Ala Asp Cys Ser His Asp Asn Ar - #g Pro Tyr Ser Phe         Ser                                                                                              715  - #               720  - #               725              - - Val Tyr Thr Pro Ser Arg Thr Cys Val Val Ty - #r Ala Pro Val Glu                       730      - #           735      - #           740                   - -  - - (2) INFORMATION FOR SEQ ID NO:16:                                     - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 2763 base - #pairs                                                 (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: Not Relev - #ant                                        - -     (ii) MOLECULE TYPE: mRNA                                               - -    (iii) HYPOTHETICAL: NO                                                  - -     (vi) ORIGINAL SOURCE:                                                           (A) ORGANISM: Zea mays                                                - -     (ix) FEATURE:                                                                   (A) NAME/KEY: transit.sub.-- - #peptide                                        (B) LOCATION: 2..190                                                  - -     (ix) FEATURE:                                                                   (A) NAME/KEY: mat.sub.-- - #peptide                                            (B) LOCATION: 191..2467                                               - -     (ix) FEATURE:                                                                   (A) NAME/KEY: CDS                                                              (B) LOCATION: 2..2470                                                 - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:16:                               - - G CTG TGC CTC GTG TCG CCC TCT TCC TCG CCG - #ACT CCG CTT CCG CCG              46                                                                           Leu Cys Leu Val Ser Pro Ser Ser Ser P - #ro Thr Pro Leu Pro Pro                -63         -60      - #           -55      - #           -50                 - - CCG CGG CGC TCT CGC TCG CAT GCT GAT CGG GC - #G GCA CCG CCG GGG ATC            94                                                                        Pro Arg Arg Ser Arg Ser His Ala Asp Arg Al - #a Ala Pro Pro Gly Ile                        -45      - #           -40      - #           -35                   - - GCG GGT GGC GGC AAT GTG CGC CTG AGT GTG TT - #G TCT GTC CAG TGC AAG           142                                                                        Ala Gly Gly Gly Asn Val Arg Leu Ser Val Le - #u Ser Val Gln Cys Lys                    -30          - #       -25          - #       -20                       - - GCT CGC CGG TCA GGG GTG CGG AAG GTC AAG AG - #C AAA TTC GCC ACT GCA           190                                                                        Ala Arg Arg Ser Gly Val Arg Lys Val Lys Se - #r Lys Phe Ala Thr Ala                -15              - #   -10              - #    -5                           - - GCT ACT GTG CAA GAA GAT AAA ACT ATG GCA AC - #T GCC AAA GGC GAT GTC           238                                                                        Ala Thr Val Gln Glu Asp Lys Thr Met Ala Th - #r Ala Lys Gly Asp Val              1               5 - #                 10 - #                 15               - - GAC CAT CTC CCC ATA TAC GAC CTG GAC CCC AA - #G CTG GAG ATA TTC AAG           286                                                                        Asp His Leu Pro Ile Tyr Asp Leu Asp Pro Ly - #s Leu Glu Ile Phe Lys                         20     - #             25     - #             30                   - - GAC CAT TTC AGG TAC CGG ATG AAA AGA TTC CT - #A GAG CAG AAA GGA TCA           334                                                                        Asp His Phe Arg Tyr Arg Met Lys Arg Phe Le - #u Glu Gln Lys Gly Ser                     35         - #         40         - #         45                       - - ATT GAA GAA AAT GAG GGA AGT CTT GAA TCT TT - #T TCT AAA GGC TAT TTG           382                                                                        Ile Glu Glu Asn Glu Gly Ser Leu Glu Ser Ph - #e Ser Lys Gly Tyr Leu                 50             - #     55             - #     60                           - - AAA TTT GGG ATT AAT ACA AAT GAG GAT GGA AC - #T GTA TAT CGT GAA TGG           430                                                                        Lys Phe Gly Ile Asn Thr Asn Glu Asp Gly Th - #r Val Tyr Arg Glu Trp             65                 - # 70                 - # 75                 - # 80        - - GCA CCT GCT GCG CAG GAG GCA GAG CTT ATT GG - #T GAC TTC AAT GAC TGG           478                                                                        Ala Pro Ala Ala Gln Glu Ala Glu Leu Ile Gl - #y Asp Phe Asn Asp Trp                             85 - #                 90 - #                 95               - - AAT GGT GCA AAC CAT AAG ATG GAG AAG GAT AA - #A TTT GGT GTT TGG TCG           526                                                                        Asn Gly Ala Asn His Lys Met Glu Lys Asp Ly - #s Phe Gly Val Trp Ser                        100      - #           105      - #           110                   - - ATC AAA ATT GAC CAT GTC AAA GGG AAA CCT GC - #C ATC CCT CAC AAT TCC           574                                                                        Ile Lys Ile Asp His Val Lys Gly Lys Pro Al - #a Ile Pro His Asn Ser                    115          - #       120          - #       125                       - - AAG GTT AAA TTT CGC TTT CTA CAT GGT GGA GT - #A TGG GTT GAT CGT ATT           622                                                                        Lys Val Lys Phe Arg Phe Leu His Gly Gly Va - #l Trp Val Asp Arg Ile                130              - #   135              - #   140                           - - CCA GCA TTG ATT CGT TAT GCG ACT GTT GAT GC - #C TCT AAA TTT GGA GCT           670                                                                        Pro Ala Leu Ile Arg Tyr Ala Thr Val Asp Al - #a Ser Lys Phe Gly Ala            145                 1 - #50                 1 - #55                 1 -       #60                                                                               - - CCC TAT GAT GGT GTT CAT TGG GAT CCT CCT GC - #T TCT GAA AGG TAC         ACA      718                                                                     Pro Tyr Asp Gly Val His Trp Asp Pro Pro Al - #a Ser Glu Arg Tyr Thr                           165  - #               170  - #               175               - - TTT AAG CAT CCT CGG CCT TCA AAG CCT GCT GC - #T CCA CGT ATC TAT GAA           766                                                                        Phe Lys His Pro Arg Pro Ser Lys Pro Ala Al - #a Pro Arg Ile Tyr Glu                        180      - #           185      - #           190                   - - GCC CAT GTA GGT ATG AGT GGT GAA AAG CCA GC - #A GTA AGC ACA TAT AGG           814                                                                        Ala His Val Gly Met Ser Gly Glu Lys Pro Al - #a Val Ser Thr Tyr Arg                    195          - #       200          - #       205                       - - GAA TTT GCA GAC AAT GTG TTG CCA CGC ATA CG - #A GCA AAT AAC TAC AAC           862                                                                        Glu Phe Ala Asp Asn Val Leu Pro Arg Ile Ar - #g Ala Asn Asn Tyr Asn                210              - #   215              - #   220                           - - ACA GTT CAG TTG ATG GCA GTT ATG GAG CAT TC - #G TAC TAT GCT TCT TTC           910                                                                        Thr Val Gln Leu Met Ala Val Met Glu His Se - #r Tyr Tyr Ala Ser Phe            225                 2 - #30                 2 - #35                 2 -       #40                                                                               - - GGG TAC CAT GTG ACA AAT TTC TTT GCG GTT AG - #C AGC AGA TCA GGC         ACA      958                                                                     Gly Tyr His Val Thr Asn Phe Phe Ala Val Se - #r Ser Arg Ser Gly Thr                           245  - #               250  - #               255               - - CCA GAG GAC CTC AAA TAT CTT GTT GAT AAG GC - #A CAC AGT TTG GGT TTG          1006                                                                        Pro Glu Asp Leu Lys Tyr Leu Val Asp Lys Al - #a His Ser Leu Gly Leu                        260      - #           265      - #           270                   - - CGA GTT CTG ATG GAT GTT GTC CAT AGC CAT GC - #A AGT AAT AAT GTC ACA          1054                                                                        Arg Val Leu Met Asp Val Val His Ser His Al - #a Ser Asn Asn Val Thr                    275          - #       280          - #       285                       - - GAT GGT TTA AAT GGC TAT GAT GTT GGA CAA AG - #C ACC CAA GAG TCC TAT          1102                                                                        Asp Gly Leu Asn Gly Tyr Asp Val Gly Gln Se - #r Thr Gln Glu Ser Tyr                290              - #   295              - #   300                           - - TTT CAT GCG GGA GAT AGA GGT TAT CAT AAA CT - #T TGG GAT AGT CGG CTG          1150                                                                        Phe His Ala Gly Asp Arg Gly Tyr His Lys Le - #u Trp Asp Ser Arg Leu            305                 3 - #10                 3 - #15                 3 -       #20                                                                               - - TTC AAC TAT GCT AAC TGG GAG GTA TTA AGG TT - #T CTT CTT TCT AAC         CTG     1198                                                                     Phe Asn Tyr Ala Asn Trp Glu Val Leu Arg Ph - #e Leu Leu Ser Asn Leu                           325  - #               330  - #               335               - - AGA TAT TGG TTG GAT GAA TTC ATG TTT GAT GG - #C TTC CGA TTT GAT GGA          1246                                                                        Arg Tyr Trp Leu Asp Glu Phe Met Phe Asp Gl - #y Phe Arg Phe Asp Gly                        340      - #           345      - #           350                   - - GTT ACA TCA ATG CTG TAT CAT CAC CAT GGT AT - #C AAT GTG GGG TTT ACT          1294                                                                        Val Thr Ser Met Leu Tyr His His His Gly Il - #e Asn Val Gly Phe Thr                    355          - #       360          - #       365                       - - GGA AAC TAC CAG GAA TAT TTC AGT TTG GAC AC - #A GCT GTG GAT GCA GTT          1342                                                                        Gly Asn Tyr Gln Glu Tyr Phe Ser Leu Asp Th - #r Ala Val Asp Ala Val                370              - #   375              - #   380                           - - GTT TAC ATG ATG CTT GCA AAC CAT TTA ATG CA - #C AAA CTC TTG CCA GAA          1390                                                                        Val Tyr Met Met Leu Ala Asn His Leu Met Hi - #s Lys Leu Leu Pro Glu            385                 3 - #90                 3 - #95                 4 -       #00                                                                               - - GCA ACT GTT GTT GCT GAA GAT GTT TCA GGC AT - #G CCG GTC CTT TGC         CGG     1438                                                                     Ala Thr Val Val Ala Glu Asp Val Ser Gly Me - #t Pro Val Leu Cys Arg                           405  - #               410  - #               415               - - CCA GTT GAT GAA GGT GGG GTT GGG TTT GAC TA - #T CGC CTG GCA ATG GCT          1486                                                                        Pro Val Asp Glu Gly Gly Val Gly Phe Asp Ty - #r Arg Leu Ala Met Ala                        420      - #           425      - #           430                   - - ATC CCT GAT AGA TGG ATT GAC TAC CTG AAG AA - #T AAA GAT GAC TCT GAG          1534                                                                        Ile Pro Asp Arg Trp Ile Asp Tyr Leu Lys As - #n Lys Asp Asp Ser Glu                    435          - #       440          - #       445                       - - TGG TCG ATG GGT GAA ATA GCG CAT ACT TTG AC - #T AAC AGG AGA TAT ACT          1582                                                                        Trp Ser Met Gly Glu Ile Ala His Thr Leu Th - #r Asn Arg Arg Tyr Thr                450              - #   455              - #   460                           - - GAA AAA TGC ATC GCA TAT GCT GAG AGC CAT GA - #T CAG TCT ATT GTT GGC          1630                                                                        Glu Lys Cys Ile Ala Tyr Ala Glu Ser His As - #p Gln Ser Ile Val Gly            465                 4 - #70                 4 - #75                 4 -       #80                                                                               - - GAC AAA ACT ATT GCA TTT CTC CTG ATG GAC AA - #G GAA ATG TAC ACT         GGC     1678                                                                     Asp Lys Thr Ile Ala Phe Leu Leu Met Asp Ly - #s Glu Met Tyr Thr Gly                           485  - #               490  - #               495               - - ATG TCA GAC TTG CAG CCT GCT TCA CCT ACA AT - #T GAT CGA GGG ATT GCA          1726                                                                        Met Ser Asp Leu Gln Pro Ala Ser Pro Thr Il - #e Asp Arg Gly Ile Ala                        500      - #           505      - #           510                   - - CTC CAA AAG ATG ATT CAC TTC ATC ACA ATG GC - #C CTT GGA GGT GAT GGC          1774                                                                        Leu Gln Lys Met Ile His Phe Ile Thr Met Al - #a Leu Gly Gly Asp Gly                    515          - #       520          - #       525                       - - TAC TTG AAT TTT ATG GGA AAT GAG TTT GGT CA - #C CCA GAA TGG ATT GAC          1822                                                                        Tyr Leu Asn Phe Met Gly Asn Glu Phe Gly Hi - #s Pro Glu Trp Ile Asp                530              - #   535              - #   540                           - - TTT CCA AGA GAA GGG AAC AAC TGG AGC TAT GA - #T AAA TGC AGA CGA CAG          1870                                                                        Phe Pro Arg Glu Gly Asn Asn Trp Ser Tyr As - #p Lys Cys Arg Arg Gln            545                 5 - #50                 5 - #55                 5 -       #60                                                                               - - TGG AGC CTT GTG GAC ACT GAT CAC TTG CGG TA - #C AAG TAC ATG AAT         GCG     1918                                                                     Trp Ser Leu Val Asp Thr Asp His Leu Arg Ty - #r Lys Tyr Met Asn Ala                           565  - #               570  - #               575               - - TTT GAC CAA GCG ATG AAT GCG CTC GAT GAG AG - #A TTT TCC TTC CTT TCG          1966                                                                        Phe Asp Gln Ala Met Asn Ala Leu Asp Glu Ar - #g Phe Ser Phe Leu Ser                        580      - #           585      - #           590                   - - TCG TCA AAG CAG ATC GTC AGC GAC ATG AAC GA - #T GAG GAA AAG GTT ATT          2014                                                                        Ser Ser Lys Gln Ile Val Ser Asp Met Asn As - #p Glu Glu Lys Val Ile                    595          - #       600          - #       605                       - - GTC TTT GAA CGT GGA GAT TTA GTT TTT GTT TT - #C AAT TTC CAT CCC AAG          2062                                                                        Val Phe Glu Arg Gly Asp Leu Val Phe Val Ph - #e Asn Phe His Pro Lys                610              - #   615              - #   620                           - - AAA ACT TAC GAG GGC TAC AAA GTG GGA TGC GA - #T TTG CCT GGG AAA TAC          2110                                                                        Lys Thr Tyr Glu Gly Tyr Lys Val Gly Cys As - #p Leu Pro Gly Lys Tyr            625                 6 - #30                 6 - #35                 6 -       #40                                                                               - - AGA GTA GCC CTG GAC TCT GAT GCT CTG GTC TT - #C GGT GGA CAT GGA         AGA     2158                                                                     Arg Val Ala Leu Asp Ser Asp Ala Leu Val Ph - #e Gly Gly His Gly Arg                           645  - #               650  - #               655               - - GTT GGC CAC GAC GTG GAT CAC TTC ACG TCG CC - #T GAA GGG GTG CCA GGG          2206                                                                        Val Gly His Asp Val Asp His Phe Thr Ser Pr - #o Glu Gly Val Pro Gly                        660      - #           665      - #           670                   - - GTG CCC GAA ACG AAC TTC AAC AAC CGG CCG AA - #C TCG TTC AAA GTC CTT          2254                                                                        Val Pro Glu Thr Asn Phe Asn Asn Arg Pro As - #n Ser Phe Lys Val Leu                    675          - #       680          - #       685                       - - TCT CCG CCC CGC ACC TGT GTG GCT TAT TAC CG - #T GTA GAC GAA GCA GGG          2302                                                                        Ser Pro Pro Arg Thr Cys Val Ala Tyr Tyr Ar - #g Val Asp Glu Ala Gly                690              - #   695              - #   700                           - - GCT GGA CGA CGT CTT CAC GCG AAA GCA GAG AC - #A GGA AAG ACG TCT CCA          2350                                                                        Ala Gly Arg Arg Leu His Ala Lys Ala Glu Th - #r Gly Lys Thr Ser Pro            705                 7 - #10                 7 - #15                 7 -       #20                                                                               - - GCA GAG AGC ATC GAC GTC AAA GCT TCC AGA GC - #T AGT AGC AAA GAA         GAC     2398                                                                     Ala Glu Ser Ile Asp Val Lys Ala Ser Arg Al - #a Ser Ser Lys Glu Asp                           725  - #               730  - #               735               - - AAG GAG GCA ACG GCT GGT GGC AAG AAG GGA TG - #G AAG TTT GCG CGG CAG          2446                                                                        Lys Glu Ala Thr Ala Gly Gly Lys Lys Gly Tr - #p Lys Phe Ala Arg Gln                        740      - #           745      - #           750                   - - CCA TCC GAT CAA GAT ACC AAA TGA AGCCACGAGT CC - #TTGGTGAG GACTGGACTG         2500                                                                        Pro Ser Asp Gln Asp Thr Lys  *                                                         755          - #       760                                              - - GCTGCCGGCG CCCTGTTAGT AGTCCTGCTC TACTGGACTA GCCGCCGCTG GC -              #GCCCTTGG   2560                                                                  - - AACGGTCCTT TCCTGTAGCT TGCAGGCGAC TGGTGTCTCA TCACCGAGCA GG -             #CAGGCACT   2620                                                                  - - GCTTGTATAG CTTTTCTAGA ATAATAATCA GGGATGGATG GATGGTGTGT AT -             #TGGCTATC   2680                                                                  - - TGGCTAGACG TGCATGTGCC CAGTTTGTAT GTACAGGAGC AGTTCCCGTC CA -             #GAATAAAA   2740                                                                  - - AAAAACTTGT TGGGGGGTTT TTC           - #                  - #                   2763                                                                      - -  - - (2) INFORMATION FOR SEQ ID NO:17:                                     - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH:  822 ami - #no acids                                               (B) TYPE: amino acid                                                           (D) TOPOLOGY: linear                                                  - -     (ii) MOLECULE TYPE: protein                                            - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:17:                               - - Leu Cys Leu Val Ser Pro Ser Ser Ser Pro Th - #r Pro Leu Pro Pro Pro      63         -60        - #         -55        - #         -50                     - - Arg Arg Ser Arg Ser His Ala Asp Arg Ala Al - #a Pro Pro Gly Ile Ala               -45          - #       -40          - #       -35                       - - Gly Gly Gly Asn Val Arg Leu Ser Val Leu Se - #r Val Gln Cys Lys Ala           -30              - #   -25              - #   -20                           - - Arg Arg Ser Gly Val Arg Lys Val Lys Ser Ly - #s Phe Ala Thr Ala Ala      15                 - - #10                  - #-5                  - # 1         - - Thr Val Gln Glu Asp Lys Thr Met Ala Thr Al - #a Lys Gly Asp Val Asp                     5    - #              10    - #              15                   - - His Leu Pro Ile Tyr Asp Leu Asp Pro Lys Le - #u Glu Ile Phe Lys Asp                20         - #         25         - #         30                       - - His Phe Arg Tyr Arg Met Lys Arg Phe Leu Gl - #u Gln Lys Gly Ser Ile            35             - #     40             - #     45                           - - Glu Glu Asn Glu Gly Ser Leu Glu Ser Phe Se - #r Lys Gly Tyr Leu Lys        50                 - # 55                 - # 60                 - # 65        - - Phe Gly Ile Asn Thr Asn Glu Asp Gly Thr Va - #l Tyr Arg Glu Trp Ala                        70 - #                 75 - #                 80               - - Pro Ala Ala Gln Glu Ala Glu Leu Ile Gly As - #p Phe Asn Asp Trp Asn                    85     - #             90     - #             95                   - - Gly Ala Asn His Lys Met Glu Lys Asp Lys Ph - #e Gly Val Trp Ser Ile               100          - #       105          - #       110                       - - Lys Ile Asp His Val Lys Gly Lys Pro Ala Il - #e Pro His Asn Ser Lys           115              - #   120              - #   125                           - - Val Lys Phe Arg Phe Leu His Gly Gly Val Tr - #p Val Asp Arg Ile Pro       130                 1 - #35                 1 - #40                 1 -       #45                                                                               - - Ala Leu Ile Arg Tyr Ala Thr Val Asp Ala Se - #r Lys Phe Gly Ala         Pro                                                                                              150  - #               155  - #               160              - - Tyr Asp Gly Val His Trp Asp Pro Pro Ala Se - #r Glu Arg Tyr Thr Phe                   165      - #           170      - #           175                   - - Lys His Pro Arg Pro Ser Lys Pro Ala Ala Pr - #o Arg Ile Tyr Glu Ala               180          - #       185          - #       190                       - - His Val Gly Met Ser Gly Glu Lys Pro Ala Va - #l Ser Thr Tyr Arg Glu           195              - #   200              - #   205                           - - Phe Ala Asp Asn Val Leu Pro Arg Ile Arg Al - #a Asn Asn Tyr Asn Thr       210                 2 - #15                 2 - #20                 2 -       #25                                                                               - - Val Gln Leu Met Ala Val Met Glu His Ser Ty - #r Tyr Ala Ser Phe         Gly                                                                                              230  - #               235  - #               240              - - Tyr His Val Thr Asn Phe Phe Ala Val Ser Se - #r Arg Ser Gly Thr Pro                   245      - #           250      - #           255                   - - Glu Asp Leu Lys Tyr Leu Val Asp Lys Ala Hi - #s Ser Leu Gly Leu Arg               260          - #       265          - #       270                       - - Val Leu Met Asp Val Val His Ser His Ala Se - #r Asn Asn Val Thr Asp           275              - #   280              - #   285                           - - Gly Leu Asn Gly Tyr Asp Val Gly Gln Ser Th - #r Gln Glu Ser Tyr Phe       290                 2 - #95                 3 - #00                 3 -       #05                                                                               - - His Ala Gly Asp Arg Gly Tyr His Lys Leu Tr - #p Asp Ser Arg Leu         Phe                                                                                              310  - #               315  - #               320              - - Asn Tyr Ala Asn Trp Glu Val Leu Arg Phe Le - #u Leu Ser Asn Leu Arg                   325      - #           330      - #           335                   - - Tyr Trp Leu Asp Glu Phe Met Phe Asp Gly Ph - #e Arg Phe Asp Gly Val               340          - #       345          - #       350                       - - Thr Ser Met Leu Tyr His His His Gly Ile As - #n Val Gly Phe Thr Gly           355              - #   360              - #   365                           - - Asn Tyr Gln Glu Tyr Phe Ser Leu Asp Thr Al - #a Val Asp Ala Val Val       370                 3 - #75                 3 - #80                 3 -       #85                                                                               - - Tyr Met Met Leu Ala Asn His Leu Met His Ly - #s Leu Leu Pro Glu         Ala                                                                                              390  - #               395  - #               400              - - Thr Val Val Ala Glu Asp Val Ser Gly Met Pr - #o Val Leu Cys Arg Pro                   405      - #           410      - #           415                   - - Val Asp Glu Gly Gly Val Gly Phe Asp Tyr Ar - #g Leu Ala Met Ala Ile               420          - #       425          - #       430                       - - Pro Asp Arg Trp Ile Asp Tyr Leu Lys Asn Ly - #s Asp Asp Ser Glu Trp           435              - #   440              - #   445                           - - Ser Met Gly Glu Ile Ala His Thr Leu Thr As - #n Arg Arg Tyr Thr Glu       450                 4 - #55                 4 - #60                 4 -       #65                                                                               - - Lys Cys Ile Ala Tyr Ala Glu Ser His Asp Gl - #n Ser Ile Val Gly         Asp                                                                                              470  - #               475  - #               480              - - Lys Thr Ile Ala Phe Leu Leu Met Asp Lys Gl - #u Met Tyr Thr Gly Met                   485      - #           490      - #           495                   - - Ser Asp Leu Gln Pro Ala Ser Pro Thr Ile As - #p Arg Gly Ile Ala Leu               500          - #       505          - #       510                       - - Gln Lys Met Ile His Phe Ile Thr Met Ala Le - #u Gly Gly Asp Gly Tyr           515              - #   520              - #   525                           - - Leu Asn Phe Met Gly Asn Glu Phe Gly His Pr - #o Glu Trp Ile Asp Phe       530                 5 - #35                 5 - #40                 5 -       #45                                                                               - - Pro Arg Glu Gly Asn Asn Trp Ser Tyr Asp Ly - #s Cys Arg Arg Gln         Trp                                                                                              550  - #               555  - #               560              - - Ser Leu Val Asp Thr Asp His Leu Arg Tyr Ly - #s Tyr Met Asn Ala Phe                   565      - #           570      - #           575                   - - Asp Gln Ala Met Asn Ala Leu Asp Glu Arg Ph - #e Ser Phe Leu Ser Ser               580          - #       585          - #       590                       - - Ser Lys Gln Ile Val Ser Asp Met Asn Asp Gl - #u Glu Lys Val Ile Val           595              - #   600              - #   605                           - - Phe Glu Arg Gly Asp Leu Val Phe Val Phe As - #n Phe His Pro Lys Lys       610                 6 - #15                 6 - #20                 6 -       #25                                                                               - - Thr Tyr Glu Gly Tyr Lys Val Gly Cys Asp Le - #u Pro Gly Lys Tyr         Arg                                                                                              630  - #               635  - #               640              - - Val Ala Leu Asp Ser Asp Ala Leu Val Phe Gl - #y Gly His Gly Arg Val                   645      - #           650      - #           655                   - - Gly His Asp Val Asp His Phe Thr Ser Pro Gl - #u Gly Val Pro Gly Val               660          - #       665          - #       670                       - - Pro Glu Thr Asn Phe Asn Asn Arg Pro Asn Se - #r Phe Lys Val Leu Ser           675              - #   680              - #   685                           - - Pro Pro Arg Thr Cys Val Ala Tyr Tyr Arg Va - #l Asp Glu Ala Gly Ala       690                 6 - #95                 7 - #00                 7 -       #05                                                                               - - Gly Arg Arg Leu His Ala Lys Ala Glu Thr Gl - #y Lys Thr Ser Pro         Ala                                                                                              710  - #               715  - #               720              - - Glu Ser Ile Asp Val Lys Ala Ser Arg Ala Se - #r Ser Lys Glu Asp Lys                   725      - #           730      - #           735                   - - Glu Ala Thr Ala Gly Gly Lys Lys Gly Trp Ly - #s Phe Ala Arg Gln Pro               740          - #       745          - #       750                       - - Ser Asp Gln Asp Thr Lys                                                       755              - #   760                                                  - -  - - (2) INFORMATION FOR SEQ ID NO:18:                                     - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 153 base - #pairs                                                  (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: Not Relev - #ant                                        - -     (ii) MOLECULE TYPE: cDNA to mRNA                                       - -    (iii) HYPOTHETICAL: NO                                                  - -     (vi) ORIGINAL SOURCE:                                                           (A) ORGANISM: Zea mays                                                - -     (ix) FEATURE:                                                                   (A) NAME/KEY: CDS                                                              (B) LOCATION: 1..153                                                  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:18:                               - - ATG GCG ACG CCC TCG GCC GTG GGC GCC GCG TG - #C CTC CTC CTC GCG CGG            48                                                                        Met Ala Thr Pro Ser Ala Val Gly Ala Ala Cy - #s Leu Leu Leu Ala Arg                            765  - #               770  - #               775               - - GCC GCC TGG CCG GCC GCC GTC GGC GAC CGG GC - #G CGC CCG CGG AGG CTC            96                                                                        Ala Ala Trp Pro Ala Ala Val Gly Asp Arg Al - #a Arg Pro Arg Arg Leu                        780      - #           785      - #           790                   - - CAG CGC GTG CTG CGC CGC CGG TGC GTC GCG GA - #G CTG AGC AGG GAG GGG           144                                                                        Gln Arg Val Leu Arg Arg Arg Cys Val Ala Gl - #u Leu Ser Arg Glu Gly                    795          - #       800          - #       805                       - - CCC CAT ATG              - #                  - #                        - #        153                                                                   Pro His Met                                                                        810                                                                         - -  - - (2) INFORMATION FOR SEQ ID NO:19:                                     - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 51 amino - #acids                                                  (B) TYPE: amino acid                                                           (D) TOPOLOGY: linear                                                  - -     (ii) MOLECULE TYPE: protein                                            - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:19:                               - - Met Ala Thr Pro Ser Ala Val Gly Ala Ala Cy - #s Leu Leu Leu Ala         Arg                                                                                1               5 - #                 10 - #                 15              - - Ala Ala Trp Pro Ala Ala Val Gly Asp Arg Al - #a Arg Pro Arg Arg Leu                    20     - #             25     - #             30                   - - Gln Arg Val Leu Arg Arg Arg Cys Val Ala Gl - #u Leu Ser Arg Glu Gly                35         - #         40         - #         45                       - - Pro His Met                                                                    50                                                                         - -  - - (2) INFORMATION FOR SEQ ID NO:20:                                     - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 1620 base - #pairs                                                 (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: double                                                       (D) TOPOLOGY: Not Relev - #ant                                        - -     (ii) MOLECULE TYPE: cDNA to mRNA                                       - -    (iii) HYPOTHETICAL: NO                                                  - -     (ix) FEATURE:                                                                   (A) NAME/KEY: CDS                                                              (B) LOCATION: 1..1620                                                 - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:20:                               - - TGC GTC GCG GAG CTG AGC AGG GAG GAC CTC GG - #T CTC GAA CCT GAA GGG            48                                                                        Cys Val Ala Glu Leu Ser Arg Glu Asp Leu Gl - #y Leu Glu Pro Glu Gly                         55     - #             60     - #             65                   - - ATT GCT GAA GGT TCC ATC GAT AAC ACA GTA GT - #T GTG GCA AGT GAG CAA            96                                                                        Ile Ala Glu Gly Ser Ile Asp Asn Thr Val Va - #l Val Ala Ser Glu Gln                     70         - #         75         - #         80                       - - GAT TCT GAG ATT GTG GTT GGA AAG GAG CAA GC - #T CGA GCT AAA GTA ACA           144                                                                        Asp Ser Glu Ile Val Val Gly Lys Glu Gln Al - #a Arg Ala Lys Val Thr                 85             - #     90             - #     95                           - - CAA AGC ATT GTC TTT GTA ACC GGC GAA GCT TC - #T CCT TAT GCA AAG TCT           192                                                                        Gln Ser Ile Val Phe Val Thr Gly Glu Ala Se - #r Pro Tyr Ala Lys Ser            100                 1 - #05                 1 - #10                 1 -       #15                                                                               - - GGG GGT CTA GGA GAT GTT TGT GGT TCA TTG CC - #A GTT GCT CTT GCT         GCT      240                                                                     Gly Gly Leu Gly Asp Val Cys Gly Ser Leu Pr - #o Val Ala Leu Ala Ala                           120  - #               125  - #               130               - - CGT GGT CAC CGT GTG ATG GTT GTA ATG CCC AG - #A TAT TTA AAT GGT ACC           288                                                                        Arg Gly His Arg Val Met Val Val Met Pro Ar - #g Tyr Leu Asn Gly Thr                        135      - #           140      - #           145                   - - TCC GAT AAG AAT TAT GCA AAT GCA TTT TAC AC - #A GAA AAA CAC ATT CGG           336                                                                        Ser Asp Lys Asn Tyr Ala Asn Ala Phe Tyr Th - #r Glu Lys His Ile Arg                    150          - #       155          - #       160                       - - ATT CCA TGC TTT GGC GGT GAA CAT GAA GTT AC - #C TTC TTC CAT GAG TAT           384                                                                        Ile Pro Cys Phe Gly Gly Glu His Glu Val Th - #r Phe Phe His Glu Tyr                165              - #   170              - #   175                           - - AGA GAT TCA GTT GAC TGG GTG TTT GTT GAT CA - #T CCC TCA TAT CAC AGA           432                                                                        Arg Asp Ser Val Asp Trp Val Phe Val Asp Hi - #s Pro Ser Tyr His Arg            180                 1 - #85                 1 - #90                 1 -       #95                                                                               - - CCT GGA AAT TTA TAT GGA GAT AAG TTT GGT GC - #T TTT GGT GAT AAT         CAG      480                                                                     Pro Gly Asn Leu Tyr Gly Asp Lys Phe Gly Al - #a Phe Gly Asp Asn Gln                           200  - #               205  - #               210               - - TTC AGA TAC ACA CTC CTT TGC TAT GCT GCA TG - #T GAG GCT CCT TTG ATC           528                                                                        Phe Arg Tyr Thr Leu Leu Cys Tyr Ala Ala Cy - #s Glu Ala Pro Leu Ile                        215      - #           220      - #           225                   - - CTT GAA TTG GGA GGA TAT ATT TAT GGA CAG AA - #T TGC ATG TTT GTT GTC           576                                                                        Leu Glu Leu Gly Gly Tyr Ile Tyr Gly Gln As - #n Cys Met Phe Val Val                    230          - #       235          - #       240                       - - AAT GAT TGG CAT GCC AGT CTA GTG CCA GTC CT - #T CTT GCT GCA AAA TAT           624                                                                        Asn Asp Trp His Ala Ser Leu Val Pro Val Le - #u Leu Ala Ala Lys Tyr                245              - #   250              - #   255                           - - AGA CCA TAT GGT GTT TAT AAA GAC TCC CGC AG - #C ATT CTT GTA ATA CAT           672                                                                        Arg Pro Tyr Gly Val Tyr Lys Asp Ser Arg Se - #r Ile Leu Val Ile His            260                 2 - #65                 2 - #70                 2 -       #75                                                                               - - AAT TTA GCA CAT CAG GGT GTA GAG CCT GCA AG - #C ACA TAT CCT GAC         CTT      720                                                                     Asn Leu Ala His Gln Gly Val Glu Pro Ala Se - #r Thr Tyr Pro Asp Leu                           280  - #               285  - #               290               - - GGG TTG CCA CCT GAA TGG TAT GGA GCT CTG GA - #G TGG GTA TTC CCT GAA           768                                                                        Gly Leu Pro Pro Glu Trp Tyr Gly Ala Leu Gl - #u Trp Val Phe Pro Glu                        295      - #           300      - #           305                   - - TGG GCG AGG AGG CAT GCC CTT GAC AAG GGT GA - #G GCA GTT AAT TTT TTG           816                                                                        Trp Ala Arg Arg His Ala Leu Asp Lys Gly Gl - #u Ala Val Asn Phe Leu                    310          - #       315          - #       320                       - - AAA GGT GCA GTT GTG ACA GCA GAT CGA ATC GT - #G ACT GTC AGT AAG GGT           864                                                                        Lys Gly Ala Val Val Thr Ala Asp Arg Ile Va - #l Thr Val Ser Lys Gly                325              - #   330              - #   335                           - - TAT TCG TGG GAG GTC ACA ACT GCT GAA GGT GG - #A CAG GGC CTC AAT GAG           912                                                                        Tyr Ser Trp Glu Val Thr Thr Ala Glu Gly Gl - #y Gln Gly Leu Asn Glu            340                 3 - #45                 3 - #50                 3 -       #55                                                                               - - CTC TTA AGC TCC AGA AAG AGT GTA TTA AAC GG - #A ATT GTA AAT GGA         ATT      960                                                                     Leu Leu Ser Ser Arg Lys Ser Val Leu Asn Gl - #y Ile Val Asn Gly Ile                           360  - #               365  - #               370               - - GAC ATT AAT GAT TGG AAC CCT GCC ACA GAC AA - #A TGT ATC CCC TGT CAT          1008                                                                        Asp Ile Asn Asp Trp Asn Pro Ala Thr Asp Ly - #s Cys Ile Pro Cys His                        375      - #           380      - #           385                   - - TAT TCT GTT GAT GAC CTC TCT GGA AAG GCC AA - #A TGT AAA GGT GCA TTG          1056                                                                        Tyr Ser Val Asp Asp Leu Ser Gly Lys Ala Ly - #s Cys Lys Gly Ala Leu                    390          - #       395          - #       400                       - - CAG AAG GAG CTG GGT TTA CCT ATA AGG CCT GA - #T GTT CCT CTG ATT GGC          1104                                                                        Gln Lys Glu Leu Gly Leu Pro Ile Arg Pro As - #p Val Pro Leu Ile Gly                405              - #   410              - #   415                           - - TTT ATT GGA AGG TTG GAT TAT CAG AAA GGC AT - #T GAT CTC ATT CAA CTT          1152                                                                        Phe Ile Gly Arg Leu Asp Tyr Gln Lys Gly Il - #e Asp Leu Ile Gln Leu            420                 4 - #25                 4 - #30                 4 -       #35                                                                               - - ATC ATA CCA GAT CTC ATG CGG GAA GAT GTT CA - #A TTT GTC ATG CTT         GGA     1200                                                                     Ile Ile Pro Asp Leu Met Arg Glu Asp Val Gl - #n Phe Val Met Leu Gly                           440  - #               445  - #               450               - - TCT GGT GAC CCA GAG CTT GAA GAT TGG ATG AG - #A TCT ACA GAG TCG ATC          1248                                                                        Ser Gly Asp Pro Glu Leu Glu Asp Trp Met Ar - #g Ser Thr Glu Ser Ile                        455      - #           460      - #           465                   - - TTC AAG GAT AAA TTT CGT GGA TGG GTT GGA TT - #T AGT GTT CCA GTT TCC          1296                                                                        Phe Lys Asp Lys Phe Arg Gly Trp Val Gly Ph - #e Ser Val Pro Val Ser                    470          - #       475          - #       480                       - - CAC CGA ATA ACT GCC GGC TGC GAT ATA TTG TT - #A ATG CCA TCC AGA TTC          1344                                                                        His Arg Ile Thr Ala Gly Cys Asp Ile Leu Le - #u Met Pro Ser Arg Phe                485              - #   490              - #   495                           - - GAA CCT TGT GGT CTC AAT CAG CTA TAT GCT AT - #G CAG TAT GGC ACA GTT          1392                                                                        Glu Pro Cys Gly Leu Asn Gln Leu Tyr Ala Me - #t Gln Tyr Gly Thr Val            500                 5 - #05                 5 - #10                 5 -       #15                                                                               - - CCT GTT GTC CAT GCA ACT GGG GGC CTT AGA GA - #T ACC GTG GAG AAC         TTC     1440                                                                     Pro Val Val His Ala Thr Gly Gly Leu Arg As - #p Thr Val Glu Asn Phe                           520  - #               525  - #               530               - - AAC CCT TTC GGT GAG AAT GGA GAG CAG GGT AC - #A GGG TGG GCA TTC GCA          1488                                                                        Asn Pro Phe Gly Glu Asn Gly Glu Gln Gly Th - #r Gly Trp Ala Phe Ala                        535      - #           540      - #           545                   - - CCC CTA ACC ACA GAA AAC ATG TTT GTG GAC AT - #T GCG AAC TGC AAT ATC          1536                                                                        Pro Leu Thr Thr Glu Asn Met Phe Val Asp Il - #e Ala Asn Cys Asn Ile                    550          - #       555          - #       560                       - - TAC ATA CAG GGA ACA CAA GTC CTC CTG GGA AG - #G GCT AAT GAA GCG AGG          1584                                                                        Tyr Ile Gln Gly Thr Gln Val Leu Leu Gly Ar - #g Ala Asn Glu Ala Arg                565              - #   570              - #   575                           - - CAT GTC AAA AGA CTT CAC GTG GGA CCA TGC CG - #C TGA                      - #     1620                                                                     His Val Lys Arg Leu His Val Gly Pro Cys Ar - #g  *                             580                 5 - #85                 5 - #90                             - -  - - (2) INFORMATION FOR SEQ ID NO:21:                                     - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH:  539 ami - #no acids                                               (B) TYPE: amino acid                                                           (D) TOPOLOGY: linear                                                  - -     (ii) MOLECULE TYPE: protein                                            - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:21:                               - - Cys Val Ala Glu Leu Ser Arg Glu Asp Leu Gl - #y Leu Glu Pro Glu         Gly                                                                                1               5 - #                 10 - #                 15              - - Ile Ala Glu Gly Ser Ile Asp Asn Thr Val Va - #l Val Ala Ser Glu Gln                    20     - #             25     - #             30                   - - Asp Ser Glu Ile Val Val Gly Lys Glu Gln Al - #a Arg Ala Lys Val Thr                35         - #         40         - #         45                       - - Gln Ser Ile Val Phe Val Thr Gly Glu Ala Se - #r Pro Tyr Ala Lys Ser            50             - #     55             - #     60                           - - Gly Gly Leu Gly Asp Val Cys Gly Ser Leu Pr - #o Val Ala Leu Ala Ala        65                 - # 70                 - # 75                 - # 80        - - Arg Gly His Arg Val Met Val Val Met Pro Ar - #g Tyr Leu Asn Gly Thr                        85 - #                 90 - #                 95               - - Ser Asp Lys Asn Tyr Ala Asn Ala Phe Tyr Th - #r Glu Lys His Ile Arg                   100      - #           105      - #           110                   - - Ile Pro Cys Phe Gly Gly Glu His Glu Val Th - #r Phe Phe His Glu Tyr               115          - #       120          - #       125                       - - Arg Asp Ser Val Asp Trp Val Phe Val Asp Hi - #s Pro Ser Tyr His Arg           130              - #   135              - #   140                           - - Pro Gly Asn Leu Tyr Gly Asp Lys Phe Gly Al - #a Phe Gly Asp Asn Gln       145                 1 - #50                 1 - #55                 1 -       #60                                                                               - - Phe Arg Tyr Thr Leu Leu Cys Tyr Ala Ala Cy - #s Glu Ala Pro Leu         Ile                                                                                              165  - #               170  - #               175              - - Leu Glu Leu Gly Gly Tyr Ile Tyr Gly Gln As - #n Cys Met Phe Val Val                   180      - #           185      - #           190                   - - Asn Asp Trp His Ala Ser Leu Val Pro Val Le - #u Leu Ala Ala Lys Tyr               195          - #       200          - #       205                       - - Arg Pro Tyr Gly Val Tyr Lys Asp Ser Arg Se - #r Ile Leu Val Ile His           210              - #   215              - #   220                           - - Asn Leu Ala His Gln Gly Val Glu Pro Ala Se - #r Thr Tyr Pro Asp Leu       225                 2 - #30                 2 - #35                 2 -       #40                                                                               - - Gly Leu Pro Pro Glu Trp Tyr Gly Ala Leu Gl - #u Trp Val Phe Pro         Glu                                                                                              245  - #               250  - #               255              - - Trp Ala Arg Arg His Ala Leu Asp Lys Gly Gl - #u Ala Val Asn Phe Leu                   260      - #           265      - #           270                   - - Lys Gly Ala Val Val Thr Ala Asp Arg Ile Va - #l Thr Val Ser Lys Gly               275          - #       280          - #       285                       - - Tyr Ser Trp Glu Val Thr Thr Ala Glu Gly Gl - #y Gln Gly Leu Asn Glu           290              - #   295              - #   300                           - - Leu Leu Ser Ser Arg Lys Ser Val Leu Asn Gl - #y Ile Val Asn Gly Ile       305                 3 - #10                 3 - #15                 3 -       #20                                                                               - - Asp Ile Asn Asp Trp Asn Pro Ala Thr Asp Ly - #s Cys Ile Pro Cys         His                                                                                              325  - #               330  - #               335              - - Tyr Ser Val Asp Asp Leu Ser Gly Lys Ala Ly - #s Cys Lys Gly Ala Leu                   340      - #           345      - #           350                   - - Gln Lys Glu Leu Gly Leu Pro Ile Arg Pro As - #p Val Pro Leu Ile Gly               355          - #       360          - #       365                       - - Phe Ile Gly Arg Leu Asp Tyr Gln Lys Gly Il - #e Asp Leu Ile Gln Leu           370              - #   375              - #   380                           - - Ile Ile Pro Asp Leu Met Arg Glu Asp Val Gl - #n Phe Val Met Leu Gly       385                 3 - #90                 3 - #95                 4 -       #00                                                                               - - Ser Gly Asp Pro Glu Leu Glu Asp Trp Met Ar - #g Ser Thr Glu Ser         Ile                                                                                              405  - #               410  - #               415              - - Phe Lys Asp Lys Phe Arg Gly Trp Val Gly Ph - #e Ser Val Pro Val Ser                   420      - #           425      - #           430                   - - His Arg Ile Thr Ala Gly Cys Asp Ile Leu Le - #u Met Pro Ser Arg Phe               435          - #       440          - #       445                       - - Glu Pro Cys Gly Leu Asn Gln Leu Tyr Ala Me - #t Gln Tyr Gly Thr Val           450              - #   455              - #   460                           - - Pro Val Val His Ala Thr Gly Gly Leu Arg As - #p Thr Val Glu Asn Phe       465                 4 - #70                 4 - #75                 4 -       #80                                                                               - - Asn Pro Phe Gly Glu Asn Gly Glu Gln Gly Th - #r Gly Trp Ala Phe         Ala                                                                                              485  - #               490  - #               495              - - Pro Leu Thr Thr Glu Asn Met Phe Val Asp Il - #e Ala Asn Cys Asn Ile                   500      - #           505      - #           510                   - - Tyr Ile Gln Gly Thr Gln Val Leu Leu Gly Ar - #g Ala Asn Glu Ala Arg               515          - #       520          - #       525                       - - His Val Lys Arg Leu His Val Gly Pro Cys Ar - #g                               530              - #   535              - #   540                           - -  - - (2) INFORMATION FOR SEQ ID NO:22:                                     - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 30 base - #pairs                                                   (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                  - -     (ii) MOLECULE TYPE: other nucleic acid                                          (A) DESCRIPTION: /desc - #= "Oligonucleotide"                         - -    (iii) HYPOTHETICAL: NO                                                  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:22:                               - - GTGGATCCAT GGCGACGCCC TCGGCCGTGG         - #                  - #                30                                                                       - -  - - (2) INFORMATION FOR SEQ ID NO:23:                                     - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 35 base - #pairs                                                   (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                  - -     (ii) MOLECULE TYPE: other nucleic acid                                          (A) DESCRIPTION: /desc - #= "Oligonucleotide"                         - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:23:                               - - CTGAATTCCA TATGGGGCCC CTCCCTGCTC AGCTC       - #                  -      #       35                                                                       - -  - - (2) INFORMATION FOR SEQ ID NO:24:                                     - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 36 base - #pairs                                                   (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                  - -     (ii) MOLECULE TYPE: other nucleic acid                                          (A) DESCRIPTION: /desc - #= "Oligonucleotide"                         - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:24:                               - - CTCTGAGCTC AAGCTTGCTA CTTTCTTTCC TTAATG      - #                  -      #       36                                                                       - -  - - (2) INFORMATION FOR SEQ ID NO:25:                                     - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 29 base - #pairs                                                   (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                  - -     (ii) MOLECULE TYPE: other nucleic acid                                          (A) DESCRIPTION: /desc - #= "Oligonucleotide"                         - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:25:                               - - GTCTCCGCGG TGGTGTCCTT GCTTCCTAG         - #                  - #                 29                                                                       - -  - - (2) INFORMATION FOR SEQ ID NO:26:                                     - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 53 base - #pairs                                                   (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: double                                                       (D) TOPOLOGY: Not Relev - #ant                                        - -     (ii) MOLECULE TYPE: cDNA to mRNA                                       - -    (iii) HYPOTHETICAL: NO                                                  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:26:                               - - TGCGTCGCGG AGCTGAGCAG GGAGGTCTCC GCGGTGGTGT CCTTGCTTCC TA - #G                 53                                                                         - -  - - (2) INFORMATION FOR SEQ ID NO:27:                                     - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 8 amino - #acids                                                   (B) TYPE: amino acid                                                           (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: Not Relev - #ant                                        - -     (ii) MOLECULE TYPE: peptide                                            - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:27:                               - -      Cys Val Ala Glu Leu Ser Arg Glu                                           1             - #  5                                                       - -  - - (2) INFORMATION FOR SEQ ID NO:28:                                     - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 base - #pairs                                                   (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: double                                                       (D) TOPOLOGY: Not Relev - #ant                                        - -     (ii) MOLECULE TYPE: cDNA to mRNA                                       - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:28:                               - - AGAGAGAGAG AGAGAG             - #                  - #                       - #    16                                                                    - -  - - (2) INFORMATION FOR SEQ ID NO:29:                                     - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 36 base - #pairs                                                   (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: double                                                       (D) TOPOLOGY: Not Relev - #ant                                        - -     (ii) MOLECULE TYPE: cDNA to mRNA                                       - -    (iii) HYPOTHETICAL: NO                                                  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:29:                               - - AAGAAGAAGA AGAAGAAGAA GAAGAAGAAG AAGAAG      - #                  -      #       36                                                                       - -  - - (2) INFORMATION FOR SEQ ID NO:30:                                     - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 18 base - #pairs                                                   (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: double                                                       (D) TOPOLOGY: Not Relev - #ant                                        - -     (ii) MOLECULE TYPE: cDNA to mRNA                                       - -    (iii) HYPOTHETICAL: NO                                                  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:30:                               - - AAAAAAAAAA AAAAAAAA             - #                  - #                       - #  18                                                                    - -  - - (2) INFORMATION FOR SEQ ID NO:31:                                     - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 11 base - #pairs                                                   (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: Not Relev - #ant                                        - -     (ii) MOLECULE TYPE: other nucleic acid                                          (A) DESCRIPTION: /desc - #= "Oligonucleotide"                         - -    (iii) HYPOTHETICAL: NO                                                  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:31:                               - - AGATAATGCA G               - #                  - #                       - #       11                                                                    - -  - - (2) INFORMATION FOR SEQ ID NO:32:                                     - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 10 base - #pairs                                                   (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: Not Relev - #ant                                        - -     (ii) MOLECULE TYPE: other nucleic acid                                          (A) DESCRIPTION: /desc - #= "Oligonucleotide"                         - -    (iii) HYPOTHETICAL: NO                                                  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:32:                               - - AACAATGGCT                - #                  - #                       - #        10                                                                    - -  - - (2) INFORMATION FOR SEQ ID NO:33:                                     - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 56 amino - #acids                                                  (B) TYPE: amino acid                                                           (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: Not Relev - #ant                                        - -     (ii) MOLECULE TYPE: peptide                                            - -    (iii) HYPOTHETICAL: NO                                                  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:33:                               - -      Met Ala Ser Ser Met Leu Ser Ser - # Ala Ala Val Ala Thr Arg         Thr Asn                                                                               1             - #  5                - #   10               - #          15                                                                                - -      Pro Ala Gln Ala Ser Met Val Ala - # Pro Phe Thr Gly Leu Lys        Ser Ala                                                                                           20 - #                 25 - #                 30              - -      Ala Phe Pro Val Ser Arg Lys Gln - # Asn Leu Asp Ile Thr Ser         Ile Ala                                                                                       35     - #             40     - #             45                   - -      Ser Asn Gly Gly Arg Val Gln Cys                                               50         - #         55                                              - -  - - (2) INFORMATION FOR SEQ ID NO:34:                                     - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 58 amino - #acids                                                  (B) TYPE: amino acid                                                           (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: Not Relev - #ant                                        - -     (ii) MOLECULE TYPE: peptide                                            - -    (iii) HYPOTHETICAL: NO                                                  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:34:                               - -      Met Ala Pro Thr Val Met Met Ala - # Ser Ser Ala Thr Ala Thr        Arg Thr                                                                               1             - #  5                - #   10               - #          15                                                                                - -      Asn Pro Ala Gln Ala Ser Ala Val - # Ala Pro Phe Gln Gly Leu        Lys Ser                                                                                           20 - #                 25 - #                 30              - -      Thr Ala Ser Leu Pro Val Ala Arg - # Arg Ser Ser Arg Ser Leu         Gly Asn                                                                                       35     - #             40     - #             45                   - -      Val Ala Ser Asn Gly Gly Arg Ile - # Arg Cys                                   50         - #         55                                              - -  - - (2) INFORMATION FOR SEQ ID NO:35:                                     - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 58 amino - #acids                                                  (B) TYPE: amino acid                                                           (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: Not Relev - #ant                                        - -     (ii) MOLECULE TYPE: peptide                                            - -    (iii) HYPOTHETICAL: NO                                                  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:35:                               - -      Met Ala Gln Ile Leu Ala Pro Ser - # Thr Gln Trp Gln Met Arg        Ile Thr                                                                               1             - #  5                - #   10               - #          15                                                                                - -      Lys Thr Ser Pro Cys Ala Thr Pro - # Ile Thr Ser Lys Met Trp        Ser Ser                                                                                           20 - #                 25 - #                 30              - -      Leu Val Met Lys Gln Thr Lys Lys - # Val Ala His Ser Ala Lys         Phe Arg                                                                                       35     - #             40     - #             45                   - -      Val Met Ala Val Asn Ser Glu Asn - # Gly Thr                                   50         - #         55                                              - -  - - (2) INFORMATION FOR SEQ ID NO:36:                                     - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 74 amino - #acids                                                  (B) TYPE: amino acid                                                           (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: Not Relev - #ant                                        - -     (ii) MOLECULE TYPE: peptide                                            - -    (iii) HYPOTHETICAL: NO                                                  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:36:                               - -      Met Ala Ala Leu Ala Thr Ser Gln - # Leu Val Ala Thr Arg Ala        Gly His                                                                               1             - #  5                - #   10               - #          15                                                                                - -      Gly Val Pro Asp Ala Ser Thr Phe - # Arg Arg Gly Ala Ala Gln        Gly Leu                                                                                           20 - #                 25 - #                 30              - -      Arg Gly Ala Arg Ala Ser Ala Ala - # Ala Asp Thr Leu Ser Met         Arg Thr                                                                                       35     - #             40     - #             45                   - -      Ser Ala Arg Ala Ala Pro Arg His - # Gln Gln Gln Ala Arg Arg        Gly Gly                                                                                   50         - #         55         - #         60                       - -      Arg Phe Pro Phe Pro Ser Leu Val - # Val Cys                               65             - #     70                                                  - -  - - (2) INFORMATION FOR SEQ ID NO:37:                                     - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 39 amino - #acids                                                  (B) TYPE: amino acid                                                           (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: Not Relev - #ant                                        - -     (ii) MOLECULE TYPE: peptide                                            - -    (iii) HYPOTHETICAL: NO                                                  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:37:                               - -      Met Ala Thr Pro Ser Ala Val Gly - # Ala Ala Cys Leu Leu Leu        Ala Arg                                                                               1             - #  5                - #   10               - #          15                                                                                - -      Xaa Ala Trp Pro Ala Ala Val Gly - # Asp Arg Ala Arg Pro Arg        Arg Leu                                                                                           20 - #                 25 - #                 30              - -      Gln Arg Val Leu Arg Arg Arg                                                       35                                                               __________________________________________________________________________ 

What is claimed is:
 1. A hybrid polypeptide comprising:(a) a starch-encapsulating region; (b) a payload polypeptide fused to said starch-encapsulating region.
 2. The hybrid polypeptide of claim 1 wherein said payload polypeptide is a biologically active polypeptide.
 3. The hybrid polypeptide of claim 2 wherein said payload polypeptide is selected from the group consisting of hormones, growth factors, antibodies, peptides, polypeptides, enzyme immunoglobulins, dyes and biologically active fragments thereof.
 4. The hybrid polypeptide of claim 1 wherein said starch-encapsulating region is the starch-encapsulating region of an enzyme selected from the group consisting of soluble starch synthase I, soluble starch synthase II, soluble starch synthase III, granule-bound starch synthase, branching enzyme I, branching enzyme IIa, branching enzyme IIBb and glucoamylase polypeptides.
 5. The hybrid polypeptide of claim 1 comprising a cleavage site between said starch-encapsulating region and said payload polypeptide.
 6. A hybrid polypeptide comprising a starch-encapsulating region of a starch-binding enzyme, wherein said starch-encapsulating region is fused to a polypeptide and said polypeptide is not endogenous to starch granules.
 7. The hybrid polypeptide of claim 6 further comprising a starch granule bound to said region.
 8. The hybrid polypeptide of claim 6 wherein said starch-encapsulating region is said starch-binding enzyme.
 9. The hybrid polypeptide of claim 6 further comprising a cleavage site between said region and said polypeptide.
 10. The hybrid polypeptide of claim 6 wherein said starch-binding enzyme is selected from the group consisting of a granule bound starch synthase, a soluble starch synthase, a glucoamylase and a branching enzyme.
 11. The hybrid polypeptide of claim 8 wherein said starch-binding enzyme is selected from the group consisting of a granule bound starch synthase, a soluble starch synthase, a glucoamylase and a branching enzyme.
 12. The hybrid polypeptide of claim 6 wherein said starch-binding enzyme is selected from the group consisting of a maize waxy protein, a rice waxy protein, a maize soluble starch synthase, a maize branching enzyme and a glycogen synthase.
 13. The hybrid polypeptide of claim 6 wherein said region is a carboxy terminal segment of said enzyme.
 14. The hybrid polypeptide of claim 8 wherein said region is a carboxy terminal segment of said enzyme.
 15. The hybrid polypeptide of claim 6 wherein said polypeptide is selected from the group consisting of a hormone, a growth factor, an immunoglobulin, an antibody, an enzyme and a dye.
 16. The hybrid polypeptide of claim 7 wherein said polypeptide is selected from the group consisting of a hormone, a growth factor, an immunoglobulin, an antibody, an enzyme and a dye.
 17. The hybrid polypeptide of claim 8 wherein said polypeptide is selected from the group consisting of a hormone, a growth factor, an immunoglobulin, an antibody, an enzyme and a dye.
 18. The hybrid polypeptide of claim 1 wherein said starch-encapsulating regions is said starch-binding enzyme.
 19. The hybrid polypeptide of claim 6 wherein said polypeptide is selected from the group consisting of somatotropin, insulin A, insulin B, calcitonin, beta endorphin, urogastrone, beta globin, myoglobin, human growth hormone, angiotensin, proline, a protease, a beta-galactosidase and a cellulase.
 20. The hybrid polypeptide of claim 9 wherein said polypeptide is selected from the group consisting of a hormone, a growth factor, an immunoglobulin, an antibody, an enzyme and a dye. 